About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:210150 Gonda Y, et al., Robo1 regulates the migration and laminar distribution of upper-layer pyramidal neurons of the cerebral cortex. Cereb Cortex. 2013 Jun;23(6):1495-508
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5752976
Gene symbol: Robo1
Gene name: roundabout guidance receptor 1
Antibody: Anti-Robo1 (AF1749)
Detection system: Biotinylated secondary antibody/[Strept]avidin
Assay notes: A tyramide signal amlification step was used.
Results
Specimen 1G: embryonic day 15.5 (more )
Note: (I) shows a higher magnification of the boxed area in (G), (K') a higher magnification of the boxed area in (K). Double labeled: green - Robo1; magenta - Satb2 (assay MGI:5754346).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex intermediate zone pyramidal neuron Strong 1G , 1I , 1K Expression is in migrating pyramidal neurons.
TS23: cortical plate Weak 1K , 1G , 1K' , 1I
Specimen 1L: embryonic day 18.5 (more )
Note: (N) shows a higher magnification of the boxed area in (L), (P') a higher magnification of the boxed area in (P). Double labeled: green - Robo1; magenta - Satb2 (assay MGI:5754346).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex marginal zone pyramidal neuron Present 1N , 1P , 1L Expression is in pyramidal neurons.
TS26: cortical plate Present Graded 1P' , 1N , 1L , 1P
Specimen 1Q: postnatal day 1 (more )
Note: (S) shows a higher magnification of the boxed area in (Q), (U') a higher magnification of the boxed area in (U). Double labeled: green - Robo1; magenta - Satb2 (assay MGI:5754346).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex marginal zone pyramidal neuron Present 1S , 1Q , 1U Expression is in pyramidal neurons.
TS27: cortical plate Present Graded 1U' , 1Q , 1S , 1U
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory