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Gene Expression Data
Assay Details
Assay
Reference: J:210150 Gonda Y, et al., Robo1 regulates the migration and laminar distribution of upper-layer pyramidal neurons of the cerebral cortex. Cereb Cortex. 2013 Jun;23(6):1495-508
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5752976
Gene symbol: Robo1
Gene name: roundabout guidance receptor 1
Antibody: Robo1 antibody
Detection system: Biotinylated secondary antibody/[Strept]avidin
Assay notes: A tyramide signal amlification step was used.
Results
Specimen 1G: embryonic day 15.5 (more )
Note: (I) shows a higher magnification of the boxed area in (G), (K') a higher magnification of the boxed area in (K). Double labeled: green - Robo1; magenta - Satb2 (assay MGI:5754346).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex intermediate zone pyramidal neuron Strong 1G , 1I , 1K Expression is in migrating pyramidal neurons.
TS23: cortical plate Weak 1K , 1G , 1K' , 1I
Specimen 1L: embryonic day 18.5 (more )
Note: (N) shows a higher magnification of the boxed area in (L), (P') a higher magnification of the boxed area in (P). Double labeled: green - Robo1; magenta - Satb2 (assay MGI:5754346).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex marginal zone pyramidal neuron Present 1N , 1P , 1L Expression is in pyramidal neurons.
TS26: cortical plate Present Graded 1P' , 1N , 1L , 1P
Specimen 1Q: postnatal day 1 (more )
Note: (S) shows a higher magnification of the boxed area in (Q), (U') a higher magnification of the boxed area in (U). Double labeled: green - Robo1; magenta - Satb2 (assay MGI:5754346).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex marginal zone pyramidal neuron Present 1S , 1Q , 1U Expression is in pyramidal neurons.
TS27: cortical plate Present Graded 1U' , 1Q , 1S , 1U
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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