Antibody:	anti-EMX1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2A	Expression first detected at E9.5 in presumptive dorsal telencephalon before any clear indication of forebrain regionalization.
TS15: genitourinary system	Present	Regionally restricted	2A	Expression in urogenital tract. Expression localized in cell nuclei.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2B	Expression in presumptive dorsal telencephalon. Anterior and posterior boundaries are not very sharp.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2C	Domain extends anterior to presumptive olfactory bulb and posteriorly to presumptive hippocampal region.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		2D	Expression localized in cell nuclei.

Structure	Level	Pattern	Image	Note
TS19: branchial pouch	Present		2E

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		3A
TS21: telencephalon	Present	Regionally restricted	3A	Expression in presumptive hippocampus.
TS21: olfactory nerve	Present	Widespread	3A	Expression along entire length from developing olfactory epithelium to the bulb.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		3B
TS22: hippocampus	Present		3B
TS22: main olfactory bulb	Present		3B
TS22: olfactory nerve	Present	Widespread	3B	Expression along entire length from developing olfactory epithelium to the bulb.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		3C
TS24: hippocampus	Present		3C
TS24: main olfactory bulb	Present		3C
TS24: olfactory nerve	Present	Widespread	3C	Expression along entire length from developing olfactory epithelium to the bulb.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		3D
TS25: hippocampus	Present		3D
TS25: main olfactory bulb	Present		3D
TS25: olfactory nerve	Present	Widespread	3D	Expression along entire length from developing olfactory epithelium to the bulb.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3E
TS28: hippocampus	Present		3E
TS28: main olfactory bulb	Present		3E
TS28: olfactory nerve	Present	Widespread	3E	Expression along entire length from developing olfactory epithelium to the bulb.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3F
TS28: dentate gyrus	Present		3F
TS28: hippocampus	Present		3F

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Widespread	4A	Expression in all cortical neurons. Expression particularly strong in ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Widespread	4B	Expression in all cortical neurons. Expression particularly strong in ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Widespread	4C	Expression in all cortical neurons.
TS21: cerebral cortex ventricular layer	Strong		4C	Expression particularly strong in ventricular zone.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Widespread	4D	Expression in all cortical neurons.
TS24: cerebral cortex ventricular layer	Strong		4D	Expression particularly strong in ventricular zone.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Widespread	4E	Expression in all cortical neurons.
TS25: cerebral cortex ventricular zone	Strong		4E	Expression particularly strong in ventricular zone.

Structure	Level	Pattern	Image	Note
TS27: cortical layer I	Absent		4F	Expression in all cortical layers except layer 1.
TS27: cortical layer II	Present		4F
TS27: cortical layer III	Present		4F
TS27: cortical layer IV	Present		4F
TS27: cortical layer V	Strong		4F	Expression stronger in forming deep layers 5 and 6 and in the subplate.
TS27: cortical layer VI	Strong		4F	Expression stronger in forming deep layers 5 and 6 and in the subplate.
TS27: cortical subplate	Strong		4F	Expression stronger in forming deep layers 5 and 6 and in the subplate.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Absent		4G	Expression in all cortical layers except layer 1.
TS28: cortical layer II	Present		4G
TS28: cortical layer III	Present		4G
TS28: cortical layer IV	Present		4G
TS28: cortical layer V	Strong		4G	Expression stronger in forming deep layers 5 and 6.
TS28: cortical layer VI	Strong		4G	Expression stronger in forming deep layers 5 and 6.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	5A	Expression in outermost layer of neuroepithelium at some distance from antero-basal border of presumptive cerebral cortex.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb	Present	Regionally restricted	5B	Expression in cell nuclei.
TS21: olfactory nerve	Present	Widespread	5B	Expression in axons of neurons of developing olfactory nerve along the entire length from olfactory epithelium to terminals touching olfactory bulb.

Structure	Level	Pattern	Image	Note
TS24: olfactory nerve	Present	Widespread	5C	Expression in axons of neurons of developing olfactory nerve.
TS24: main olfactory bulb mitral cell layer	Present	Regionally restricted	5C	Expression in nuclei of proliferating cells.
TS24: main olfactory bulb prospective granule cell layer	Present	Regionally restricted	5C	Expression in nuclei of proliferating cells.
TS24: main olfactory bulb olfactory nerve layer	Present		5C

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		5D
TS28: main olfactory bulb granule cell layer	Present		5D
TS28: main olfactory bulb mitral cell layer	Present		5D
TS28: main olfactory bulb olfactory nerve layer	Present		5D

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb olfactory nerve layer	Present		5E
TS28: main olfactory bulb	Present	Regionally restricted	5E	Expression in external neuropil where terminal of olfactory never synapse dendrites of axons deriving from bulbar cells of mitral and tufted types.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Weak	Regionally restricted	5F	Faint expression in nuclei of some mitral cells.
TS28: main olfactory bulb glomerular layer	Present	Regionally restricted	5F	Expression in spheroid or ellipsoid structures called glomeruli.
TS28: olfactory nerve	Present	Regionally restricted	5F	Expression in terminals of axons.

Structure	Level	Pattern	Image	Note
TS19: forebrain-midbrain boundary region	Present	Regionally restricted	5G	Expression in nuclei of individual cells located at boundary between diencephalon and mesencephalon.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	5H	Expression in posterior mesencephalon.

Structure	Level	Pattern	Image	Note
TS25: midbrain	Present	Regionally restricted	5I	Expression in posterior mesencephalon.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present		5J

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Present		5K

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		5L

Structure	Level	Pattern	Image	Note
TS27: olfactory nerve	Present		6A