Antibody:

Anti-Cyclin D2 (Ab-4 MS-221)

Structure	Level	Pattern	Image	Note
TS21: lens	Present		1A
TS21: retina	Present	Regionally restricted	1A	Expression was confined to the exit zone of the optic nerve and the anterior margin of the retina.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		1E
TS21: cerebral cortex ventricular layer	Present	Regionally restricted	1E	Expression was highest at the top of the cortical ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Absent		1F
TS21: cerebral cortex ventricular layer	Absent		1F

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present	Regionally restricted	1I	Expression was highest in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Absent		1J

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1M	Expression was detected in the ventricular zone of the hippocampal formation.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Absent		1N

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	2A	Expression was primarily distributed in the abventricular ventricular zone removed from the ventricular surface.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		2D
TS22: cortical plate	Present	Regionally restricted	2D	Expression was detected in processes that were reminiscent of glial fibers with end-feet at the pial surface.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular zone	Present		2G
TS25: cortical plate	Absent		2G

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Regionally restricted	3A	Immunoreactivity was in the subventricular zone and nonhomogeneously present in nuclei scattered in the ventricular zone.
TS20: medial ganglionic eminence	Present	Regionally restricted	3A	Immunoreactivity was in the subventricular zone and nonhomogeneously present in nuclei scattered in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Regionally restricted	3D	Expression was robust in the subventricular zone, scattered in the ventricular zone, and lowest in tissue bordering the interganglionic sulcus.

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Present	Regionally restricted	4A , 4C	Expression was primarily in the striatal ventricular zone. Immunolabeled cells were pale or absent from the ventricular surface, and labeling was greater in the subventricular zone. Immunopositive neurons were scattered in the striatum.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5A	Only a few immunoreactive cells are labeled in the hippocampal ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	5D	Expression was detected in the ammonic ventricular zone.
TS22: telencephalon	Present	Regionally restricted	5D	Expression was detected in the cortical hem.

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present	Regionally restricted	6A	Expression was detected in the ventricular zone.
TS26: hippocampus	Present	Regionally restricted	6A	Expression was detected in the ventricular zone.
TS26: hippocampal formation	Present	Regionally restricted	6A	Expression was detected in migrating cells of the dentate matrix migratory stream.

Structure	Level	Pattern	Image	Note
TS26: hippocampal formation	Present	Regionally restricted	6C	Expression was detected in migrating neuroblasts of the dentate migratory stream.

Structure	Level	Pattern	Image	Note
TS26: hippocampal formation	Present	Regionally restricted	6D	Expression was detected in migrating neuroblasts of the dentate migratory stream.
TS26: hippocampal fissure	Strong		6D

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus hilus	Present		7C , 7A
TS28: CA3 pyramidal cell layer	Moderate		7A
TS28: hippocampus region	Present	Scattered	7A

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7E	Expression in the mutant was similar to wild type.
TS28: hippocampus	Present		7E	Expression in the mutant was similar to wild type.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		8A

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Absent		8B

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		8C	Expression was increased in the mutant.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Absent		8I
TS28: dentate gyrus granule cell layer	Present		8I
TS28: hippocampus CA3	Present		8I

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	S2B' , S2B	A few immunoreactive nuclei were found in the cortical gray matter. Expression was found in processes in the superficial layers.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Single cells	S2E	Only rare scattered immunopositive cells were noted at P7, and processes were no longer labeled.

Structure	Level	Pattern	Image	Note
TS27: rostral migratory stream	Present

Structure	Level	Pattern	Image	Note
TS28: cerebral hemisphere white matter	Present		S3D , S3A

Structure	Level	Pattern	Image	Note
TS28: cerebral hemisphere white matter	Absent		S3B

Structure	Level	Pattern	Image	Note
TS28: cerebral hemisphere white matter	Present	Clusters	S3E , S3C

Structure	Level	Pattern	Image	Note
TS20: caudal ganglionic eminence	Present	Regionally restricted	S5A	Expression was localized mainly in the ventricular zone/subventricular zone and processes of the lateral caudal ganglionic eminence. Label was absent in the medial region.

Structure	Level	Pattern	Image	Note
TS22: caudal ganglionic eminence	Present	Regionally restricted	S5C	Expression was greatest in the ventricular zone/subventricular zone and processes of the lateral caudal ganglionic eminence. Label was reduced in the central region, but was visible in the most medial region.

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Present	Regionally restricted	S6A	Immunolabeled nuclei were abundant along the ventricular wall and most robust at the corticostriatal junction.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present	Regionally restricted	S6C	The ventricular zone/subventricular zone expression decreased at P7.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Ambiguous	Single cells		Immunopositive nuclei were infrequently observed at the ventricular surface.

Structure	Level	Pattern	Image	Note
TS25: hippocampal formation	Present	Regionally restricted	S7B , S7C	Expression was detected in the ventricular zone. A modest number of cells were immunopositive in the dentate migratory stream.
TS25: dentate gyrus	Moderate		S7C , S7B
TS25: hippocampal fissure	Present	Punctate	S7B