Antibody:	Brn-2 antibody
Detection system:	Secondary antibody
Assay notes:	The antibody used in this assay recognizes two family members.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	1A	Graded expression in neural progenitors. Expression in intermediate neural progenitors only in lateral most region of cortex.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	1A'	Expression spreads medially in ventricular zone. Expression in intermediate neural precursors.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	1B	Graded expression in neural progenitors.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	1B'	Expression spreads medially.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	1C	Expression in cells at all phases of differentiation.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	1D	Expression sweeps across lateral-to-medial axis between E12.5 to E14.5. Expression in upper layer neurons; no expression in deep layers.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Regionally restricted	1D'	Expression sweeps across lateral-to-medial axis between E12.5 to E14.5. Expression in intermediate zone, manifests in cortical plate with a lagging medial boundary.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	1D''	Expression sweeps across lateral-to-medial axis between E12.5 to E14.5.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	1D'''	Authors report stable expression by E15.5. Expression in intermediate zone, manifests in cortical plate with a lagging medial boundary.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present		S1A

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		S1B

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present		S1C

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	S2B	Expression in cells migrating into layers II/III of cortical plate, and cells that might represent a population of layer V corticospinal motor neurons.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	S2B'	Expression in cells migrating into layers II/III of cortical plate, and cells that might represent a population of layer V corticospinal motor neurons.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	S2B''	Expression in cells migrating into layers II/III of cortical plate, and cells that might represent a population of layer V corticospinal motor neurons.

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Present	Regionally restricted	S2D	Expression in upper portions of layer V.
TS27: central nervous system	Present	Regionally restricted	S2D	Expression in corticospinal motor neurons.

Structure	Level	Pattern	Image	Note
TS27: cortical layer VI	Present	Regionally restricted	S2D'	Expression in upper portions of layer V.
TS27: central nervous system	Present	Regionally restricted	S2D'	Expression in corticospinal motor neurons.

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Present	Regionally restricted	S2D''	Expression in upper portions of layer V.
TS27: central nervous system	Present	Regionally restricted	S2D''	Expression in corticospinal motor neurons.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	S3A Merge , S3A Brn1/2	Expression in migrating upper layer cells.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	S3C Brn1/2 , S3C Merge	Expression in migrating upper layer cells.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	S3E Merge , S3E Brn1/2	Expression in upper layer cells.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	S3G Merge , S3G Brn1/2	Expression in upper layer cells.