Probe:	Ezrin probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1A	Expression at dorsal midline of telencephalic and diencephalic vesicles, where prosencephalic choroid plexus will develop in the roof plate.
TS15: telencephalon	Present	Regionally restricted	1A	Expression at dorsal midline of telencephalic and diencephalic vesicles, where prosencephalic choroid plexus will develop in the roof plate.
TS15: midbrain-hindbrain junction	Present		1A	Expression in the isthmic region.
TS15: future hindbrain roof plate	Present	Regionally restricted	1A	Caudally, expression in expanded roof of fourth ventricle, in presumptive cerebellar region and presumptive area of rhombencephalic choroid plexus.
TS15: otic pit	Present		1A	Expression in otic placode.
TS15: umbilical cord	Present		1A
TS15: notochord	Present	Regionally restricted	1A	Expression in caudal tip of notochord.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	1B	Expression in choroid plexus anlage in telencephalon, diencephalon and rhombencephalon.
TS17: hindbrain	Present	Regionally restricted	1B	Expression in choroid plexus anlage in telencephalon, diencephalon and rhombencephalon.
TS17: telencephalon	Present	Regionally restricted	1B	Expression in choroid plexus anlage in telencephalon, diencephalon and rhombencephalon.
TS17: midbrain	Weak	Regionally restricted	1B	Very weak expression in caudal mesencephalon
TS17: midbrain-hindbrain junction	Weak		1B	Very weak expression in isthmus.

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Weak		1C	Weak expression in ventricular zone at isthmic region.
TS19: cerebellum primordium	Weak	Regionally restricted	1C	Weak expression in ventricular zone at dorsal midline of cerebellar plate.
TS19: hindbrain roof plate	Present	Regionally restricted	1C	Expression in choroid plexus anlage.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Strong	Regionally restricted	1D	Intense expression in the medial ganglionic eminence.
TS20: diencephalon	Present	Regionally restricted	1D	Two transversal domains in diencephalon alar plate.
TS20: zona limitans intrathalamica	Present	Regionally restricted	1D	Rostral domain at anterior and posterior edges of zona limitans intrathalamic.
TS20: prosomere 1	Present	Regionally restricted	1D	Caudal domain at limit between pretectum and dorsal thalamus (prosomeres 1 and 2).
TS20: prosomere 2	Present	Regionally restricted	1D	Caudal domain at limit between pretectum and dorsal thalamus (prosomeres 1 and 2).
TS20: hypothalamus	Present	Regionally restricted	1D	Expression in ventral thalamus and hypothalamus corresponds to a basal longitudinal domain that expands ventrally to completely cover the basal plate in the mammillary region, ending abruptly at limit between mammillary region and infundibular region.
TS20: prethalamus	Present	Regionally restricted	1D	Expression in ventral thalamus and hypothalamus corresponds to a basal longitudinal domain that expands ventrally to completely cover the basal plate in the mammillary region, ending abruptly at limit between mammillary region and infundibular region.
TS20: cerebellum primordium	Present	Regionally restricted	1D	Caudal alar expression domain includes caudal basal plate of mesencephalon and midline of cerebellar plate, and corresponds to more extentive domain in basal plate that follows rostral from rhombencephalon to contact the mes-diencephalic limit.
TS20: midbrain	Present	Regionally restricted	1D	Caudal alar expression domain includes caudal basal plate of mesencephalon and midline of cerebellar plate, and corresponds to more extentive domain in basal plate that follows rostral from rhombencephalon to contact the mes-diencephalic limit.

Structure	Level	Pattern	Image	Note
TS23: 4th ventricle choroid plexus	Strong		1E
TS23: lateral ventricle choroid plexus	Strong		1E

Structure	Level	Pattern	Image	Note
TS23: lateral ventricle choroid plexus	Strong		1F

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2A	Expression in ventricular epithelium of choroid plexus regions.
TS20: lateral ventricle choroid plexus	Present	Regionally restricted	2A	Expression in ventricular epithelium of choroid plexus regions.
TS20: midbrain	Weak	Regionally restricted	2A	Weak expression in caudal mesencephalon.
TS20: midbrain-hindbrain junction	Weak	Regionally restricted	2A	Weak expression in isthmic epithelium.
TS20: cerebellum primordium	Weak	Regionally restricted	2A	Weak expression in middle of cerebellar plate.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2B	Expression in ventricular epithelium of choroid plexus regions.
TS20: lateral ventricle choroid plexus	Present	Regionally restricted	2B	Expression in ventricular epithelium of choroid plexus regions.
TS20: midbrain	Weak	Regionally restricted	2B	Weak expression in caudal mesencephalon.
TS20: midbrain-hindbrain junction	Weak	Regionally restricted	2B	Weak expression in isthmic epithelium.
TS20: cerebellum primordium	Weak	Regionally restricted	2B	Weak expression in middle of cerebellar plate.

Structure	Level	Pattern	Image	Note
TS20: lateral ventricle choroid plexus	Present	Regionally restricted	2C	Ventricular expression.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Regionally restricted	2D	Ventricular expression.
TS20: midbrain	Weak	Regionally restricted	2D	Weak expression in caudal mesencephalon.
TS20: midbrain-hindbrain junction	Weak	Regionally restricted	2D	Weak expression in isthmic epithelium.
TS20: cerebellum primordium	Weak	Regionally restricted	2D	Weak expression in middle of cerebellar plate.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		2E
TS21: lateral ventricle choroid plexus	Present		2E
TS21: diencephalon	Present	Regionally restricted	2E	Expression in anterior basal plate of diencephalon.
TS21: hypothalamus	Present	Regionally restricted	2E	Expression in caudal hypothalamus.
TS21: midbrain	Weak	Regionally restricted	2E	Weak expression in caudal mesencephalon.
TS21: midbrain-hindbrain junction	Weak	Regionally restricted	2E	Weak expression in isthmic epithelium.
TS21: cerebellum	Weak	Regionally restricted	2E	Weak expression in middle of cerebellar plate.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2F	Expression in anterior basal plate of diencephalon.
TS21: hypothalamus	Present	Regionally restricted	2F	Expression in caudal hypothalamus.
TS21: midbrain	Weak	Regionally restricted	2F	Weak expression in caudal mesencephalon.
TS21: midbrain-hindbrain junction	Weak	Regionally restricted	2F	Weak expression in isthmic epithelium.
TS21: cerebellum	Weak	Regionally restricted	2F	Weak expression in middle of cerebellar plate.
TS21: 4th ventricle choroid plexus	Present		2F

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	2G	Expression in medial cortex at level of cortical hem.
TS21: medial ganglionic eminence	Weak	Regionally restricted	2G	Weak expression in neuroepithelium of medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Weak	Regionally restricted	2H	Weak expression in neuroepithelium of medial and lateral ganglionic eminences.
TS21: medial ganglionic eminence	Weak	Regionally restricted	2H	Weak expression in neuroepithelium of medial and lateral ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	2I	Expression in anterior basal plate of diencephalon.
TS22: hypothalamus	Present	Regionally restricted	2I	Expression in caudal hypothalamus.
TS22: midbrain	Weak	Regionally restricted	2I	Weak expression in caudal mesencephalon.
TS22: cerebellum	Weak	Regionally restricted	2I	Weak expression in middle of cerebellar plate.
TS22: 4th ventricle choroid plexus	Present		2I

Structure	Level	Pattern	Image	Note
TS22: lateral ventricle choroid plexus	Present		2J

Structure	Level	Pattern	Image	Note
TS22: lateral ventricle choroid plexus	Present		2K
TS22: hippocampus	Present	Regionally restricted	2K	Expression in dentate gyrus of hippocampus.
TS22: diencephalon	Present	Regionally restricted	2K	Expression in anterior basal plate of diencephalon.
TS22: midbrain	Weak	Regionally restricted	2K	Weak expression in caudal mesencephalon.
TS22: 4th ventricle choroid plexus	Present		2K

Structure	Level	Pattern	Image	Note
TS22: midbrain	Weak	Regionally restricted	2L	Weak expression in caudal mesencephalon.
TS22: cerebellum	Weak	Regionally restricted	2L	Weak expression in middle of cerebellar plate.
TS22: 4th ventricle choroid plexus	Present		2L

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	2M	Expression in anterior basal plate of diencephalon.
TS23: midbrain	Weak	Regionally restricted	2M	Weak expression in caudal mesencephalon.
TS23: midbrain-hindbrain junction	Weak	Regionally restricted	2M	Weak expression in isthmic epithelium.
TS23: cerebellum	Weak	Regionally restricted	2M	Weak expression in middle of cerebellar plate.
TS23: 4th ventricle choroid plexus	Present		2M

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Strong	Regionally restricted	2N	Strong expression in telencephalic neuroepithelium.
TS23: diencephalon	Present	Regionally restricted	2N	Expression in anterior basal plate of diencephalon
TS23: hypothalamus	Present	Regionally restricted	2N	Expression in caudal hypothalamus.
TS23: midbrain	Weak	Regionally restricted	2N	Weak expression in caudal mesencephalon.
TS23: midbrain-hindbrain junction	Weak	Regionally restricted	2N	Weak expression in isthmic epithelium.
TS23: cerebellum	Weak	Regionally restricted	2N	Weak expression in middle of cerebellar plate.
TS23: 4th ventricle choroid plexus	Present		2N

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Strong	Regionally restricted	2O	Strong expression in telencephalic neuroepithelium.
TS23: diencephalon	Present	Regionally restricted	2O	Expression in anterior basal plate of diencephalon
TS23: hypothalamus	Present	Regionally restricted	2O	Expression in caudal hypothalamus.
TS23: midbrain	Weak	Regionally restricted	2O	Weak expression in caudal mesencephalon.
TS23: midbrain-hindbrain junction	Weak	Regionally restricted	2O	Weak expression in isthmic epithelium.
TS23: cerebellum	Weak	Regionally restricted	2O	Weak expression in middle of cerebellar plate.
TS23: 4th ventricle choroid plexus	Present		2O

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Strong	Regionally restricted	2P	Strong expression in telencephalic neuroepithelium.
TS23: choroid plexus	Present		2P

Structure	Level	Pattern	Image	Note
TS26: preoptic area	Present	Regionally restricted	3A	Expression in epithelium of preoptic area and pallidum (medial ganglionic eminence).
TS26: medial ganglionic eminence	Present	Regionally restricted	3A
TS26: hypothalamus	Present	Regionally restricted	3A	Expression in ventricular epithelium of ventral thalamus and hypothalamus.
TS26: prethalamus	Present	Regionally restricted	3A	Expression in ventricular epithelium of ventral thalamus and hypothalamus.
TS26: diencephalon	Present	Regionally restricted	3A	Band of expressing epithelium follows dorsal part of basal plate, extending from mammillary region caudally towards diencephalic-mesencephalic limit.
TS26: midbrain	Present	Regionally restricted	3A	Expression in mesencephalic epithelium showed two bands at each side of ventricular wall covering whole mesencephalon and following rostrally into diencephalic region.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	3B	Domain in mammillary region follows caudally into band until caudal diencephalic epithelium.
TS26: hypothalamus	Present		3B
TS26: prethalamus	Present		3B
TS26: midbrain	Present	Regionally restricted	3B	Expression in mesencephalic epithelium showed two bands at each side of ventricular wall covering whole mesencephalon and following rostrally into diencephalic region.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	3B'	Domain in mammillary region follows caudally into band until caudal diencephalic epithelium.
TS26: hypothalamus	Present		3B'
TS26: preoptic area	Present		3B'
TS26: prethalamus	Present		3B'
TS26: midbrain	Present	Regionally restricted	3B'	Expression in mesencephalic epithelium showed two bands at each side of ventricular wall covering whole mesencephalon and following rostrally into diencephalic region.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	3C	Expression in dorsal telencephalon and periventricular neuroepithelium of lateral ventricle.
TS26: rostral migratory stream	Present		3C
TS26: cortical plate	Present		3C
TS26: dentate gyrus	Present		3C
TS26: hippocampus	Present		3C
TS26: medial ganglionic eminence	Present		3C	Expression in pallidum (medial ganglionic eminence).

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	3D	Expression in dorsal telencephalon and periventricular neuroepithelium of lateral ventricle. Expression in telencephalic epithelium.
TS26: dentate gyrus	Present		3D	Expression in medial hippocampal complex corresponds to dentate gyrus.
TS26: hippocampus	Present		3D	Expression in medial hippocampal complex corresponds to dentate gyrus.

Structure	Level	Pattern	Image	Note
TS26: rostral migratory stream	Present	Regionally restricted	3E

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	3F	Expression in dorsal telencephalon and periventricular neuroepithelium of lateral ventricle. Intense zone of expression at lateral angle of lateral ventricle, formed between corpus callosum and striatum.
TS26: hippocampus	Present		3F
TS26: hypothalamus	Present		3F
TS26: prethalamus	Present		3F

Structure	Level	Pattern	Image	Note
TS26: hypothalamus	Present	Regionally restricted	3G	Expression in ventricular epithelium of ventral thalamus and hypothalamus.
TS26: prethalamus	Present	Regionally restricted	3G	Expression in ventricular epithelium of ventral thalamus and hypothalamus.

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present		3H	Expression localized in periventricular cells of aqueduct.

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Present		3I	Expression localized in periventricular cells of aqueduct.
TS26: 4th ventricle choroid plexus	Present		3I

Structure	Level	Pattern	Image	Note
TS27: 3rd ventricle choroid plexus	Present		3J
TS27: 4th ventricle choroid plexus	Present		3J
TS27: lateral ventricle choroid plexus	Present		3J
TS27: hippocampus	Present		3J
TS27: hypothalamus	Present		3J

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present	Regionally restricted	3K	Expression in telencephalic ventricular epithelium, both in pallium and subpallium, stronger than at previous stages.
TS27: dentate gyrus	Present		3K
TS27: hippocampus	Present		3K
TS27: hypothalamus	Present		3K
TS27: 4th ventricle choroid plexus	Present		3K
TS27: lateral ventricle choroid plexus	Present		3K

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present		3L

Structure	Level	Pattern	Image	Note
TS27: lateral ventricle choroid plexus	Present		3M

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		3N
TS27: hypothalamus	Present		3N

Structure	Level	Pattern	Image	Note
TS27: 4th ventricle choroid plexus	Present		3O
TS27: cerebellum external granule cell layer	Present		3O
TS27: hypothalamus	Present		3O
TS27: lateral ventricle choroid plexus	Present		3O

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		3P
TS27: lateral ventricle choroid plexus	Present		3P

Structure	Level	Pattern	Image	Note
TS27: 4th ventricle choroid plexus	Present		3Q
TS27: cerebellum external granule cell layer	Present		3Q

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	3R	Expression localized in calloso-striatal angle of lateral ventricles and ventricular epithelium of striatum, continuing rostral and ventrally in cells of rostral migratory stream.
TS28: rostral migratory stream	Present		3R
TS28: corpus striatum	Present		3R

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present	Regionally restricted	3S	Expression in hypothalamic epithelium of third ventricle.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Weak	Regionally restricted	3T	Weak expression in anterior region of hippocampus.
TS28: hypothalamus	Present	Regionally restricted	3T	Expression in hypothalamic epithelium of third ventricle.