Gene Expression Data
Assay Details
Assay
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Reference:
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J:84136
Heuer H, et al., Connective tissue growth factor: a novel marker of layer VII neurons in the rat cerebral cortex. Neuroscience. 2003;119(1):43-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5750780
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Gene symbol:
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Ccn2
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Gene name:
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cellular communication network factor 2
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Probe:
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Ctgf cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Frozen sections were fixed with 4% formaldehyde.
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Results
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Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
NMRI
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Age:
|
embryonic day 12.5
|
Age Note:
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Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: brain |
Present |
Regionally restricted |
3A
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Expression is in the neuroepithelial layer and the meninges.
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Specimen
3B:
embryonic day 14.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3C:
embryonic day 16.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
3D:
embryonic day 18.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
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Embedding:
|
Cryosection
|
Specimen
3E:
postnatal day 0
(more )
Specimen
3E:
(close )
Genetic Background:
|
NMRI
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Age:
|
postnatal day 0
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
Fresh Frozen
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: neocortex layer |
Strong |
Regionally restricted |
3E
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Expression is in cortical layer VII.
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Specimen
3F:
postnatal day 7
(more )
Specimen
3F:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal day 7
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Fresh Frozen
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Strong |
Regionally restricted |
3F
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Expression is in cortical layer VII.
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Specimen
3G:
postnatal day 14
(more )
Specimen
3G:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal day 14
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Fresh Frozen
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Strong |
Regionally restricted |
3G
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Expression is in cortical layer VII.
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Specimen
3H:
postnatal day 21
(more )
Specimen
3H:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Strong |
Regionally restricted |
3H
|
Expression is in cortical layer VII.
|
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