Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:159518
Kuramochi-Miyagawa S, et al., MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 2010 May;24(9):887-92
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5750092
|
|
Gene symbol:
|
Piwil4
|
|
Gene name:
|
piwi-like RNA-mediated gene silencing 4
|
|
Antibody:
|
anti-MIWI2
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 568
|
|
|
Results
|
Specimen
4A WT:
embryonic day 16.5
(more  )
Specimen
4A WT:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of testis |
Present |
Spotted |
4A MIWI2 WT
|
Expressed in the nucleus, and a few larger granules in the cytoplasm, presumably corresponding to the processing bodies.
|
Specimen
4A MVH-KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Ddx4tm1Tnc/Ddx4tm1Tnc
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of testis |
Present |
|
4A MIWI2 MVH-KO
|
No expression in the nucleus, and those larger granules were not detectable.
|
Specimen
4A MILI-KO:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Piwil2tm1Nkn/Piwil2tm1Nkn
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4A MIWI2-KO:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd
|
|
Mutant Allele(s):
|
Piwil4tm1Ghan/Piwil4tm1Ghan
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools
