St8sia4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:47237 Ong E, et al., Developmental regulation of polysialic acid synthesis in mouse directed by two polysialyltransferases, PST and STX. Glycobiology. 1998 Apr;8(4):415-24
Assay type:	RNA in situ
MGI Accession ID:	MGI:5749831
Gene symbol:	St8sia4
Gene name:	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4

Probe:	mPST cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Hybrid-Ready tissue slides were purchased from Novagen.

Results

Specimen 5 - E8: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: neural crest	Present		5_E8 PST
TS12: neural tube	Present		5_E8 PST

Specimen 5 - E9: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: future telencephalon	Present	Regionally restricted	5_E9 PST	Expressed in the ventricular zone of the lateral ventricles.
TS14: future spinal cord	Present	Regionally restricted	5_E9 PST	Expressed in the ventricular zone.
TS14: mesencephalic vesicle	Present	Regionally restricted	5_E9 PST	Expressed in the ventricular zone.
TS14: future hindbrain	Present	Regionally restricted	5_E9 PST	Expressed in the ventricular zone of the 4th ventricle.

Specimen 5 - E10: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: future hindbrain ventricular layer	Present	Regionally restricted	5_E10 PST	Expressed in the ventricular zone of the 4th ventricle.
TS16: future spinal cord	Present	Regionally restricted	5_E10 PST	Expressed in the ventricular zone.
TS16: mesencephalic vesicle	Present	Regionally restricted	5_E10 PST	Expressed in the ventricular zone.
TS16: telencephalon	Present	Regionally restricted	5_E10 PST	Expressed in the ventricular zone of the lateral ventricles.
TS16: heart	Present		5_E10 PST
TS16: liver	Present		5_E10 PST

Specimen 5 - E11: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	5_E11 PST	Expressed in the ventricular zone.
TS18: lateral ventricle	Present	Regionally restricted	5_E11 PST	Expressed in the ventricular zone.
TS18: mesencephalic vesicle	Present	Regionally restricted	5_E11 PST	Expressed in the ventricular zone.
TS18: hindbrain ventricular layer	Present	Regionally restricted	5_E11 PST	Expressed in the ventricular zone of the 4th ventricle.
TS18: dorsal root ganglion	Present		5_E11 PST
TS18: lung	Present		5_E11 PST
TS18: mesonephros	Present		5_E11 PST

Specimen 5 - E12: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: lateral ventricle	Present	Regionally restricted	5 Continued_E12 PST	Expressed in the ventricular zone.
TS20: mesencephalic vesicle	Present	Regionally restricted	5 Continued_E12 PST	Expressed in the ventricular zone.
TS20: intestine	Present		5 Continued_E12 PST
TS20: metanephros	Present		5 Continued_E12 PST
TS20: pancreas	Present		5 Continued_E12 PST
TS20: spinal cord ventricular layer	Present		5 Continued_E12 PST
TS20: tongue	Present		5 Continued_E12 PST
TS20: hindbrain ventricular layer	Present	Regionally restricted	5 Continued_E12 PST	Expressed in the ventricular zone of the 4th ventricle.
TS20: embryo	Present		5 Continued_E12 PST	Highest expression.
TS20: gland	Present	Regionally restricted	5 Continued_E12 PST	Expressed in the adrenal gland.

Specimen 5 - E13: embryonic day 13.0 (more

)

Structure	Level	Pattern	Image	Note
TS21: brain	Present		5 Continued_E13 PST
TS21: spinal cord	Present		5 Continued_E13 PST

Specimen 5 - E14: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Present		5 Continued_E14 PST
TS22: thyroid gland	Present		5 Continued_E14 PST

Specimen 5 - E15: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: lateral ventricle	Present		5 Continued_E15 PST

Specimen 6: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: lateral ventricle	Present	Regionally restricted	6_PST	Expressed in the ventricular zone.
TS20: mesencephalic vesicle	Present	Regionally restricted	6_PST	Expressed in the ventricular zone.
TS20: hindbrain ventricular layer	Present	Regionally restricted	6_PST	Expressed in the ventricular zone of the 4th ventricle.
TS20: gland	Present	Regionally restricted	6_PST	Expressed in the adrenal gland.
TS20: intestine	Present		6_PST
TS20: metanephros	Present		6_PST
TS20: pancreas	Present		6_PST
TS20: spinal cord ventricular layer	Present		6_PST
TS20: tongue	Present		6_PST

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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