Gene Expression Data
Assay Details
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Assay
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Reference:
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J:152650
Ivey KN, et al., Transcriptional regulation during development of the ductus arteriosus. Circ Res. 2008 Aug 15;103(4):388-95
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5708366
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Gene symbol:
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Edn1
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Gene name:
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endothelin 1
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Probe:
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Et-1 probe3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3b:
embryonic day 13.5
(more  )
Specimen
3b:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: blood vessel endothelium |
Present |
Widespread |
3b
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Expression in endothelial cells throughout the developing vasculature.
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| TS21: ductus arteriosus |
Strong |
Regionally restricted |
3b
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Enriched expression in ductal smooth muscle, with distinct borders at the aortic and pulmonary artery junctions.
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Specimen
3c:
embryonic day 18.5
(more )
Specimen
3c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: blood vessel endothelium |
Present |
Widespread |
3c
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Expression in endothelial cells throughout the developing vasculature.
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| TS26: ductus arteriosus |
Absent |
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3c
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Expression in ductal smooth muscle was indistinguishable from background.
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Specimen
3d:
postnatal day 0
(more )
Specimen
3d:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Age Note:
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15 minutes after birth
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3e:
postnatal day 0
(more )
Specimen
3e:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Age Note:
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30 minutes after birth
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5e:
embryonic day 13.5
(more )
Specimen
5e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Tfap2btm1Rbu/Tfap2btm1Rbu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: ductus arteriosus |
Weak |
Regionally restricted |
5f
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Expression diminished in ductal smooth muscle. Lower expression in mutants compared with wild type embryos.
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| TS21: blood vessel endothelium |
Present |
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5f
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Expression not diminished in endothelial cells.
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Analysis Tools