Kcnj6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:193529 Aguado C, et al., Differential maturation of GIRK2-expressing neurons in the mouse cerebellum. J Chem Neuroanat. 2013 Jan;47:79-89
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5707495
Gene symbol:	Kcnj6
Gene name:	potassium inwardly-rectifying channel, subfamily J, member 6

Antibody:	Anti-Girk2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 2A: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong	Regionally restricted	2A	Intense expression was detected in the inner part of the external granule cell layer. The outer part was not stained.
TS27: cerebellum internal granule cell layer	Strong		2A
TS27: cerebellum Purkinje cell layer	Absent		2A

Specimen 2B: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong	Regionally restricted	2B	Intense expression was detected in the inner part of the external granule cell layer. The outer part was not stained.
TS27: cerebellum internal granule cell layer	Strong		2B
TS27: cerebellum molecular layer	Strong	Regionally restricted	2B	Expression was strong in granule cells traversing the molecular layer.
TS27: cerebellum Purkinje cell layer	Absent		2B

Specimen 2C: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	2C	Intense expression was detected in the inner part of the external granule cell layer. The outer part was not stained.
TS28: cerebellum internal granule cell layer	Strong		2C
TS28: cerebellum Purkinje cell layer	Absent		2C

Specimen 2D: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	2D	Intense expression was detected in the inner part of the external granule cell layer. The outer part was not stained.
TS28: cerebellum internal granule cell layer	Strong		2D
TS28: cerebellum Purkinje cell layer	Absent		2D

Specimen 2E: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	2E	Intense expression was detected in the inner part of the external granule cell layer. The outer part was not stained.
TS28: cerebellum internal granule cell layer	Strong		2E
TS28: cerebellum molecular layer	Strong	Regionally restricted	2E	Strong expression was detected in granule cells traversing the molecular layer.
TS28: cerebellum Purkinje cell layer	Absent		2E

Specimen 2F: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	2F	Expression was strong in the inner part of the external granule cell layer.
TS28: cerebellum internal granule cell layer	Strong		2F

Specimen 2G: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	2G	Expression was strong in the inner part of the external granule cell layer.
TS28: cerebellum internal granule cell layer	Strong		2G

Specimen 2H: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	2H	Expression was strong in the inner part of the external granule cell layer.
TS28: cerebellum internal granule cell layer	Strong		2H
TS28: cerebellum molecular layer	Present	Regionally restricted	2H	Fewer immunoreactive granule cells were crossing the molecular layer than at earlier stages.

Specimen 2I: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present		2I	Fewer immunoreactive granule cells were crossing the molecular layer than at earlier stages. Immunoreactivity was located in the leading elongated process and in some cells in the nascent parallel fibers.

Specimen 2J: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		2J
TS28: cerebellum molecular layer	Moderate		2J

Specimen 2K: postnatal day 60 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		2K

Specimen 2L: postnatal day 60 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		2L

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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