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Gene Expression Data
Assay Details
Assay
Reference: J:187589 Grunewald E, et al., Developmental expression of orphan g protein-coupled receptor 50 in the mouse brain. ACS Chem Neurosci. 2012 Jun 20;3(6):459-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5707010
Gene symbol: Gpr50
Gene name: G-protein-coupled receptor 50
Antibody: anti-GPR50 (sc-50590)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: piriform cortex Present 3A , 3A Gpr50
Specimen 3B: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 3B Gpr50 , 3B
Specimen 3C: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: hypothalamus Present 3C Gpr50 , 3C
Specimen 3D: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: superior colliculus Present 3D , 3D Gpr50
Specimen 3E: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: hippocampus Present 3E , 3E Gpr50
Specimen 3F: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: brain gray matter Present 3F , 3F Gpr50 Expression is in the pontine grey area.
Specimen 3G: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: lateral reticular nucleus Present Regionally restricted 3G Gpr50 , 3G Expression is in the magnocellular reticular nucleus.
Specimen 3H: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: lateral reticular nucleus Present Regionally restricted 3H , 3H Gpr50 Expression is in the parvocellular reticular nucleus.
Specimen 3I: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: trigeminal V sensory nucleus Present 3I Gpr50 , 3I
Specimen 3J: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Rbfox3 (assay MGI:5707012).
Structure Level Pattern Image Note
TS28: locus coeruleus Present 3J Gpr50 , 3J
Specimen 4A: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Dbh (assay MGI:5707014).
Structure Level Pattern Image Note
TS28: locus coeruleus Strong 4A Gpr50 , 4A
Specimen 4E: postnatal adult (more )
Note: Fixative was 70% ethanol. Double labeled: green - Gpr50; red - Th (assay MGI:5707015).
Structure Level Pattern Image Note
TS28: substantia nigra Present 4E Gpr50 , 4E
Specimen 5A: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: hypothalamus Present Regionally restricted 5A Gpr50 , 5A Expression is in the subparaventricular zone.
Specimen 5B: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present 5B , 5B Gpr50
TS28: hypothalamus Present Regionally restricted 5B , 5B Gpr50 Expression is in the ventromedial hypothalamus.
Specimen 5C: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: dorsomedial hypothalamic nucleus Present 5C , 5C Gpr50
Specimen 5D: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: hypothalamus lateral zone Present 5D , 5D Gpr50
Specimen 5E: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: amygdala Present 5E , 5E Gpr50
Specimen 5F: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: superior colliculus Present 5F , 5F Gpr50
Specimen 5G: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: primary somatosensory cortex Present 5G , 5G Gpr50
Specimen 5H: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS28: trigeminal V mesencephalic nucleus Present 5H Gpr50 , 5H
Specimen 6A: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS26: forebrain Present 6A Gpr50 , 6A' , 6A' Gpr50 , 6A
Specimen 6B: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS26: frontal cortex Present 6B' Gpr50 , 6B , 6B' , 6B Gpr50
Specimen 6C: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS26: midbrain Present 6C Gpr50 , 6C' , 6C' Gpr50 , 6C
Specimen 6D: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: green - Gpr50; red - Rtn4 (assay MGI:5707022).
Structure Level Pattern Image Note
TS26: medulla oblongata Present 6D' Gpr50 , 6D' , 6D , 6D Gpr50
Specimen 7A: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: 3rd ventricle ependyma Present 7A , 7A Gpr50
TS28: adenohypophysis Present 7A , 7A Gpr50
TS28: median eminence Present 7A , 7A Gpr50
Specimen 7B: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: 3rd ventricle ependyma Present 7B , 7B Gpr50
Specimen 7C: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: 3rd ventricle ependyma Present Regionally restricted 7C , 7C Gpr50 Expression is in the anterior hypothalamus.
TS28: hypothalamus Present Regionally restricted 7C , 7C Gpr50 Expression is in the anterior hypothalamus.
Specimen 7D: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: piriform cortex Present 7D , 7D Gpr50
Specimen 7E: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: primary somatosensory cortex Present 7E , 7E Gpr50
Specimen 7F: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: trigeminal V sensory nucleus Present 7F Gpr50 , 7F
Specimen 7G: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: gigantocellular reticular nucleus Present 7G Gpr50 , 7G
Specimen 7H: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: lateral reticular nucleus Present Regionally restricted 7H , 7H Gpr50 Expression is in parvocellular reticular nucleus.
Specimen 7I: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: locus coeruleus Present 7I , 7I Gpr50
TS28: trigeminal V mesencephalic nucleus Present 7I , 7I Gpr50
Specimen 7J: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Abca2 (assay MGI:5707030).
Structure Level Pattern Image Note
TS28: raphe magnus nucleus Present 7J , 7J Gpr50
Specimen 8A: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Cdh8 (assay MGI:5707032).
Structure Level Pattern Image Note
TS28: median eminence Present 8A Gpr50 , 8A
Specimen 8B: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Cdh8 (assay MGI:5707032).
Structure Level Pattern Image Note
TS28: hypothalamus periventricular zone Present 8B , 8B Gpr50
Specimen 8C: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Cdh8 (assay MGI:5707032).
Structure Level Pattern Image Note
TS28: amygdala Present 8C Gpr50 , 8C
Specimen 8D: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: green - Gpr50; red - Cdh8 (assay MGI:5707032).
Structure Level Pattern Image Note
TS28: trigeminal V mesencephalic nucleus Present 8D Gpr50 , 8D
Specimen 9A: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: green - Gpr50; red - Cdh8 (assay MGI:5707032).
Structure Level Pattern Image Note
TS26: forebrain Present Regionally restricted 9A' Gpr50 , 9A Gpr50 , 9A' , 9A Expression is in the dorsal forebrain.
Specimen 9B: embryonic day 18.5 (more )
Note: Fixative was Methacarn.
Structure Level Pattern Image Note
TS26: 3rd ventricle Present 9B' Gpr50 , 9B' , 9B Gpr50 , 9B
TS26: median eminence Present 9B' Gpr50 , 9B' , 9B Gpr50 , 9B
Specimen 9C: embryonic day 18.5 (more )
Note: Fixative was Methacarn.
Structure Level Pattern Image Note
TS26: cerebral cortex Present 9C Gpr50 , 9C' Gpr50 , 9C' , 9C
Specimen 9D: embryonic day 18.5 (more )
Note: Fixative was Methacarn.
Structure Level Pattern Image Note
TS26: hindbrain roof Present Regionally restricted 9D , 9D' , 9D Gpr50 , 9D' Gpr50 Expression is in the pontine/medullary hindbrain.
Specimen S2A SN: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol.
Structure Level Pattern Image Note
TS28: substantia nigra Present S2A SN bottom
Specimen S2A 3V: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol.
Structure Level Pattern Image Note
TS28: diencephalon Present S2A 3V bottom
Specimen S2A NRM: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol.
Structure Level Pattern Image Note
TS28: raphe magnus nucleus Present S2A NRM bottom
Specimen S2A PARN: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol.
Structure Level Pattern Image Note
TS28: lateral reticular nucleus Present Regionally restricted S2A PARN bottom Expression is in parvocellular reticular nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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