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Gene Expression Data
Assay Details
Assay
Reference: J:187589 Grunewald E, et al., Developmental expression of orphan g protein-coupled receptor 50 in the mouse brain. ACS Chem Neurosci. 2012 Jun 20;3(6):459-72
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5707022
Gene symbol: Rtn4
Gene name: reticulon 4
Antibody: anti-Nogo-A (sc-25660)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5A: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: hypothalamus Present Regionally restricted 5A , 5A Nogo-A Expression is in the subparaventricular zone.
Specimen 5B: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present 5B Nogo-A , 5B
TS28: hypothalamus Present Regionally restricted 5B Nogo-A , 5B Expression is in the ventromedial hypothalamus.
Specimen 5C: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: dorsomedial hypothalamic nucleus Present 5C Nogo-A , 5C
Specimen 5D: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: hypothalamus lateral zone Present 5D , 5D Nogo-A
Specimen 5E: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: hypothalamus Present 5E Nogo-A , 5E
Specimen 5F: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: superior colliculus Present 5F Nogo-A , 5F
Specimen 5G: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: primary somatosensory cortex Present 5G , 5G Nogo-A
Specimen 5H: postnatal adult (more )
Note: Fixative was Methacarn or 70% ethanol. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS28: trigeminal V mesencephalic nucleus Present 5H , 5H Nogo-A
Specimen 6A: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS26: forebrain Present 6A Nogo-A , 6A' , 6A' Nogo-A , 6A
Specimen 6B: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS26: frontal cortex Present 6B' , 6B Nogo-A , 6B' Nogo-A , 6B
Specimen 6C: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS26: midbrain Present 6C' Nogo-A , 6C , 6C' , 6C Nogo-A
Specimen 6D: embryonic day 18.5 (more )
Note: Fixative was Methacarn. Double labeled: red - Rtn4; green - Gpr50 (assay MGI:5707010).
Structure Level Pattern Image Note
TS26: medulla oblongata Present 6D , 6D' Nogo-A , 6D Nogo-A , 6D'
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory