Col1a1<tm2(tetO-Ccnb2)Jvd> Targeted Allele Detail MGI Mouse (MGI:5705291)

Col1a1^{tm2(tetO-Ccnb2)Jvd}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm2(tetO-Ccnb2)Jvd}
Name:	collagen, type I, alpha 1; targeted mutation 2, Jan M A van Deursen
MGI ID:	MGI:5705291
Synonyms:	Ccnb2-HA, Ccnb2^T15, Ccnb2^T16
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm2(tetO-Ccnb2)Jvd} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:219399
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Inducible, Inserted expressed sequence)

Inducer:

doxycycline/tetracycline

Mutation:

Insertion

Col1a1^{tm2(tetO-Ccnb2)Jvd} expresses 1 gene

Mutation details: RMCE on KH2 cells replaced the FRT-flanked neo cassette with a cassette that contains a splice acceptor, polyadenylation sequence and the tetracycline promoter driving inducible expression of the tetracycline promoter and an HA-tagged cDNA for mouse Ccnb2, polyadenylation sequence and hygromycin resistance cassette with an FRT site. (J:219399)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm3(neo/hygro*))Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:219399 Nam HJ, et al., Cyclin B2 and p53 control proper timing of centrosome separation. Nat Cell Biol. 2014 Jun;16(6):538-49
All:	1 reference(s)

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