Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:94531
Nieto M, et al., Expression of Cux-1 and Cux-2 in the subventricular zone and upper layers II-IV of the cerebral cortex. J Comp Neurol. 2004 Nov 8;479(2):168-80
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5702591
|
|
Gene symbol:
|
Cux2
|
|
Gene name:
|
cut-like homeobox 2
|
|
Probe:
|
Cux2 probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
6A:
embryonic day 13.5
(more  )
Specimen
6A:
(close  )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cortical plate |
Present |
|
6A
|
Expression in emerging population of upper layer neurons migrating in and on top ot the cortical plate.
|
Specimen
6B:
embryonic day 15.5
(more )
Specimen
6B:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical plate |
Present |
|
6B
|
Expression in emerging population of upper layer neurons migrating in and on top ot the cortical plate.
|
Specimen
6C:
embryonic day 11.5
(more )
Specimen
6C:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
6C
|
Expression first detected at E12 in lateral cortex. No expression in cells of the cortical plate.
|
Specimen
6D:
embryonic day 13.5
(more )
Specimen
6D:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex subventricular zone |
Present |
Regionally restricted |
6D
|
Expression restricted to the subventricular zone and to the emerging population of upper layer neurons migrating in and on top of the cortical plate.
|
| TS21: cortical plate |
Present |
Regionally restricted |
6D
|
Expression restricted to the subventricular zone and to the emerging population of upper layer neurons migrating in and on top of the cortical plate.
|
Specimen
6E:
embryonic day 15.5
(more )
Specimen
6E:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
6F:
embryonic day 18.5
(more )
Specimen
6F:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
not shown:
postnatal newborn
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
7A,C:
embryonic day 15.5
(more )
Note: Double labeled: purple - Cux2; red - BrdU.
Specimen
7A,C:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - Cux2; red - BrdU.
|
Specimen
7E:
embryonic day 15.5
(more )
Specimen
7E:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
7G:
embryonic day 15.5
(more )
Specimen
7G:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
8E:
embryonic day 13.5
(more )
Specimen
8E:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
8F:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Weak |
|
8F
|
Authors report progressive reduction of expression.
|
Specimen
8G:
embryonic day 15.5
(more )
Specimen
8G:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
8H:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Weak |
|
8H
|
Authors report progressive reduction of expression.
|
|
Analysis Tools