Antibody:	EphA3 (C-19) antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Present		2B
TS20: ganglionic eminence	Present		2B
TS20: corpus striatum	Present	Regionally restricted	2B	Expressed in the caudate-putamen.

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Present		2F , 2D
TS20: ganglionic eminence	Present		2F , 2D

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present		3B
TS21: thalamus	Present	Regionally restricted	3B	Expressed in thalamic eminence.
TS21: telencephalon	Present	Regionally restricted	3B	Expressed in internal capsule.
TS21: lateral ganglionic eminence	Present		3B
TS21: olfactory cortex	Present	Regionally restricted	3B	Expressed in lateral olfactory tract.
TS21: corpus striatum	Present	Regionally restricted	3B	Expressed in caudate-putamen.
TS21: epithalamus	Present	Regionally restricted	3B	Expressed in stria medullaris.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present		3E
TS21: thalamus	Present	Regionally restricted	3E	Expressed in thalamic eminence.
TS21: telencephalon	Present	Regionally restricted	3E	Expressed in internal capsule.
TS21: lateral ganglionic eminence	Present		3E
TS21: olfactory cortex	Present	Regionally restricted	3E	Expressed in lateral olfactory tract.
TS21: corpus striatum	Present	Regionally restricted	3E	Expressed in caudate-putamen.
TS21: epithalamus	Present	Regionally restricted	3E	Expressed in stria medullaris.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		4D	Expressed in fibers, which were continuous with the immunopositive fibers in the internal capsule, external capsule and the lateral olfactory tract.
TS22: thalamus	Present	Regionally restricted	4D	Expressed in thalamic eminence.
TS22: epithalamus	Present	Regionally restricted	4D	Expressed in stria medullaris.
TS22: telencephalon	Present	Regionally restricted	4D	Expressed in internal and external capsule.
TS22: olfactory cortex	Present	Regionally restricted	4D	Expressed in lateral olfactory tract.
TS22: optic chiasma	Present		4D

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		4E
TS22: optic chiasma	Present		4E
TS22: thalamus	Present	Regionally restricted	4E	Expressed in thalamic eminence.
TS22: epithalamus	Present	Regionally restricted	4E	Expressed in stria medullaris.
TS22: telencephalon	Present	Regionally restricted	4E	Expressed in internal and external capsule.
TS22: olfactory cortex	Present	Regionally restricted	4E	Expressed in lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		4G
TS22: optic chiasma	Present		4G
TS22: thalamus	Present	Regionally restricted	4G	Expressed in thalamic eminence.
TS22: epithalamus	Present	Regionally restricted	4G	Expressed in stria medullaris.
TS22: telencephalon	Present	Regionally restricted	4G	Expressed in internal and external capsule.
TS22: olfactory cortex	Present	Regionally restricted	4G	Expressed in lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Strong		5B

Structure	Level	Pattern	Image	Note
TS23: olfactory cortex	Strong	Regionally restricted	5D	Expressed in lateral olfactory tract.
TS23: diencephalon	Strong	Regionally restricted	5D	Expressed in anterior commissure.

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Strong	Regionally restricted	5F	Expressed in internal and external capsule.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Strong	Regionally restricted	6C	Expressed in anterior commissure.
TS24: cerebral internal capsule	Strong		6C
TS24: olfactory tract	Strong	Regionally restricted	6C	Expressed in lateral olfactory tract.
TS24: epithalamus	Strong	Regionally restricted	6C	Expressed in stria medullaris.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb mitral cell layer	Present		6E
TS24: main olfactory bulb	Present		6E
TS24: olfactory tract	Present	Regionally restricted	6E	Expressed in lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Strong	Regionally restricted	6I	Expression seemed to be split into the upper and lower parts, especially in the dorsal cortex, and the pattern and strength were different between each other.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Strong	Regionally restricted	6J	Expression seemed to be split into the upper and lower parts, especially in the dorsal cortex, and the pattern and strength were different between each other.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		7C
TS26: epithalamus	Present	Regionally restricted	7C	Expressed in stria medullaris.
TS26: hippocampus	Present	Regionally restricted	7C	Expressed in fimbria hippocampus.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present		7F

Structure	Level	Pattern	Image	Note
TS27: olfactory tract	Present	Regionally restricted	8B	Expressed in lateral olfactory tract.
TS27: epithalamus	Present	Regionally restricted	8B	Expressed in stria medullaris.
TS27: fimbria hippocampus	Present		8B
TS27: cerebral hemisphere white matter	Present	Regionally restricted	8B	Expressed in the external capsule.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex intermediate zone	Present		8D

Structure	Level	Pattern	Image	Note
TS23: epithalamus	Strong	Regionally restricted		Expressed in stria medullaris, as those at E14.
TS23: thalamus	Strong	Regionally restricted		Expressed in thalamic eminence, as those at E14.
TS23: optic chiasma	Strong			As those at E14.

Structure	Level	Pattern	Image	Note
TS24: cerebral hemisphere white matter	Strong	Regionally restricted		Expressed in the external capsule.
TS24: thalamus	Strong	Regionally restricted		Expressed in thalamic eminence.
TS24: hippocampus	Strong	Regionally restricted		Expressed in fimbria hippocampus.

Structure	Level	Pattern	Image	Note
TS26: anterior commissure	Present
TS26: telencephalon	Present	Regionally restricted		Expressed in the external capsule.
TS26: olfactory tract	Present	Regionally restricted		Expressed in lateral olfactory tract.
TS26: brain	Present	Regionally restricted		Expressed in corpus callosum.

Structure	Level	Pattern	Image	Note
TS27: anterior commissure	Present
TS27: corpus callosum	Present