Gene Expression Data
Assay Details
Assay
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Reference:
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J:99710
Kudo C, et al., Expression profiles of EphA3 at both the RNA and protein level in the developing mammalian forebrain. J Comp Neurol. 2005 Jul 4;487(3):255-69
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5699568
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Gene symbol:
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Epha3
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Gene name:
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Eph receptor A3
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Antibody:
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EphA3 (C-19) antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
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Results
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Specimen
1B:
embryonic day 14.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
9A/C/Q:
embryonic day 14.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
9A/C/Q:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Specimen
9E/G/R:
embryonic day 14.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
9E/G/R:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Specimen
9I/K/S:
embryonic day 16.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
9I/K/S:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Specimen
9M/O/T:
embryonic day 18.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
9M/O/T:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Specimen
10A/C:
embryonic day 14.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
10A/C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: telencephalon |
Present |
Regionally restricted |
10A
,
10C
|
Coexpressed with L1cam in corpus callosum.
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Specimen
10D/F:
embryonic day 16.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
10D/F:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Specimen
10G/I:
embryonic day 18.5
(more )
Note: Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
Specimen
10G/I:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - L1cam (assay MGI:5699587).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: telencephalon |
Present |
Regionally restricted |
10G
,
10I
|
Coexpressed with L1cam in corpus callosum.
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Specimen
11A/C/Q:
embryonic day 14.5
(more )
Note: Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
Specimen
11A/C/Q:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
|
Specimen
11E/G/R:
embryonic day 14.5
(more )
Note: Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
Specimen
11E/G/R:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
|
Specimen
11I/K/S:
embryonic day 16.5
(more )
Note: Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
Specimen
11I/K/S:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex intermediate zone |
Present |
Regionally restricted |
11I
,
11S
,
11K
|
Stained diffusely and weakly in the upper region, and intensively stained in the lower region. Lower region was colabeled with Cntn2.
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Specimen
11M/O/T:
embryonic day 18.5
(more )
Note: Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
Specimen
11M/O/T:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Epha3; purple - Cntn2 (assay MGI:5699588).
|
|