Runx2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:220612 Holmes G, et al., BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development. Gene Expr Patterns. 2015 Jan;17(1):16-25
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5699546
Gene symbol:	Runx2
Gene name:	runt related transcription factor 2

Antibody:	anti-RUNX2 (HPA022040)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1B2 ©

1B3 ©

Specimen 1 B2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: premaxilla	Present		1B2 , 1B3	Expression is in osteoprogenitors and mature osteoblasts.
TS22: face mesenchyme	Absent		1B3 , 1B2

1C2 ©

1C3 ©

Specimen 1 C2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Regionally restricted	1C3 , 1C2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: premaxilla	Present	Regionally restricted	1C3 , 1C2	Expression is in osteoprogenitors and mature osteoblasts.

1D2 ©

1D3 ©

Specimen 1 D2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		1D2 , 1D3	Expression is in osteoprogenitors and mature osteoblasts.
TS22: palatal shelf	Present		1D2 , 1D3	Expression is in osteoprogenitors and mature osteoblasts.

1E2 ©

1E3 ©

Specimen 1 E2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: mandible	Present		1E3 , 1E2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: face mesenchyme	Absent		1E3 , 1E2

1F2 ©

1F3 ©

Specimen 1 F2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: inter-parietal bone primordium	Present		1F3 , 1F2	Expression is in osteoprogenitors and mature osteoblasts.

2B2 ©

2B3 ©

Specimen 2 B2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: nasal septum	Present	Regionally restricted	2B2 , 2B3	Expression is in the vomer bones of the nasal septum.

2C2 ©

2C3 ©

Specimen 2 C2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		2C3 , 2C2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: palatal shelf	Present		2C3 , 2C2	Expression is in osteoprogenitors and mature osteoblasts.

2D2 ©

2D3 ©

Specimen 2 D2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		2D3 , 2D2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: palatal shelf	Present		2D3 , 2D2	Expression is in osteoprogenitors and mature osteoblasts.

2E2 ©

2E3 ©

Specimen 2 E2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		2E3 , 2E2	Expression is in osteoprogenitors and mature osteoblasts, absent in the anterior part.
TS22: palatal shelf	Present		2E3 , 2E2	Expression is in osteoprogenitors and mature osteoblasts, absent in the anterior part.

2F2 ©

2F3 ©

Specimen 2 F2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: nose	Present	Regionally restricted	2F3 , 2F2	Expression is in osteoprogenitors and mature osteoblasts.

2G2 ©

2G3 ©

Specimen 2 G2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: frontal bone primordium	Present		2G3 , 2G2	Expression is in osteoprogenitors and mature osteoblasts. Expression is reduced at the edges.

3B2 ©

3B3 ©

Specimen 3 B2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: face mesenchyme	Absent		3B3 , 3B2

3C2 ©

3C3 ©

Specimen 3 C2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: nose	Present	Regionally restricted	3C3 , 3C2	Expression is in osteoprogenitors and mature osteoblasts of the nasal bones.
TS24: frontal bone	Present	Regionally restricted	3C3 , 3C2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: premaxilla	Present	Regionally restricted	3C3 , 3C2	Expression is in osteoprogenitors and mature osteoblasts.

3D2 ©

3D3 ©

Specimen 3 D2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	3D2 , 3D3	Expression is in osteoprogenitors and mature osteoblasts.
TS24: premaxilla	Present	Regionally restricted	3D2 , 3D3	Expression is in osteoprogenitors and mature osteoblasts.

3E2 ©

3E3 ©

Specimen 3 E2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	3E3 , 3E2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: secondary palate	Present	Regionally restricted	3E3 , 3E2	Expression is in osteoprogenitors and mature osteoblasts.

3F2 ©

3F3 ©

Specimen 3 F2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Regionally restricted	3F3 , 3F2	Expression is in osteoprogenitors and mature osteoblasts.

4B2 ©

4B3 ©

Specimen 4 B2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	4B3 , 4B2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: secondary palate	Present	Regionally restricted	4B3 , 4B2	Expression is in osteoprogenitors and mature osteoblasts.

4C2 ©

4C3 ©

Specimen 4 C2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Regionally restricted	4C3 , 4C2	Expression is in osteoprogenitors and mature osteoblasts.

4D2 ©

4D3 ©

Specimen 4 D2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Regionally restricted	4D3 , 4D2	Expression is in osteoprogenitors and mature osteoblasts.

4E2 ©

4E3 ©

Specimen 4 E2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present	Regionally restricted	4E2 , 4E3	Expression is in osteoprogenitors and mature osteoblasts.

4F2 ©

4F3 ©

Specimen 4 F2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	4F2 , 4F3	Expression is in osteoprogenitors and mature osteoblasts in the zygomatic process of maxillary bone.

4G2 ©

4G3 ©

Specimen 4 G2: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: jaw skeleton	Present	Regionally restricted	4G2 , 4G3	Expression is in osteoprogenitors and mature osteoblasts.

5A2 ©

5A3 ©

Specimen 5 A2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: parietal bone	Present	Regionally restricted	5A2 , 5A3	Expression is in osteoprogenitors and mature osteoblasts.

5B2 ©

5B3 ©

Specimen 5 B2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present	Regionally restricted	5B2 , 5B3	Expression is in osteoprogenitors and mature osteoblasts.

5C2 ©

5C3 ©

Specimen 5 C2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present	Regionally restricted	5C3 , 5C2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: parietal bone	Present	Regionally restricted	5C3 , 5C2	Expression is in osteoprogenitors and mature osteoblasts.

5D2 ©

5D3 ©

Specimen 5 D2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: parietal bone	Present	Regionally restricted	5D3 , 5D2	Expression is in osteoprogenitors and mature osteoblasts and is weak in the squamo-parietal suture.
TS24: temporal bone	Present	Regionally restricted	5D3 , 5D2	Expression is in osteoprogenitors and mature osteoblasts and is weak in the squamo-parietal suture.

6B2 ©

6B3 ©

Specimen 6 B2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: nasal bone	Present	Regionally restricted	6B2 , 6B3	Expression is in osteoprogenitors and mature osteoblasts, and is absent at the edges.
TS26: premaxilla	Present	Regionally restricted	6B2 , 6B3	Expression is in osteoprogenitors and mature osteoblasts, and is absent at the edges.

6C2 ©

6C3 ©

Specimen 6 C2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: premaxilla	Present	Regionally restricted	6C3 , 6C2	Expression is in osteoprogenitors and mature osteoblasts.

6D2 ©

6D3 ©

Specimen 6 D2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: nasal bone	Present	Regionally restricted	6D2 , 6D3	Expression is in osteoprogenitors and mature osteoblasts.

6E2 ©

6E3 ©

Specimen 6 E2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: frontal bone	Present	Regionally restricted	6E2 , 6E3	Expression is in osteoprogenitors and mature osteoblasts.
TS26: parietal bone	Present	Regionally restricted	6E2 , 6E3	Expression is in osteoprogenitors and mature osteoblasts.

6F2 ©

6F3 ©

Specimen 6 F2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: frontal bone	Present	Regionally restricted	6F3 , 6F2	Expression is in osteoprogenitors and mature osteoblasts.

7B ©

Specimen 7B: embryonic day 16.5 (more

)
Note: Double labeled with alkaline phosphatase (red).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	7B	Expression is in osteoprogenitors and mature osteoblasts in the zyogomatic process of the maxillary bone.

7D ©

Specimen 7D: embryonic day 16.5 (more

)
Note: Double labeled with alkaline phosphatase (red).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	7D	Expression is in osteoprogenitors and mature osteoblasts.
TS24: secondary palate	Present	Regionally restricted	7D	Expression is in osteoprogenitors and mature osteoblasts.

7F ©

Specimen 7F: embryonic day 16.5 (more

)
Note: Double labeled with alkaline phosphatase (red).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	7F	Expression is in osteoprogenitors and mature osteoblasts.

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