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Gene Expression Data
Assay Details
Assay
Reference: J:220612 Holmes G, et al., BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development. Gene Expr Patterns. 2015 Jan;17(1):16-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5699414
Gene symbol: Bcl11b
Gene name: B cell leukemia/lymphoma 11B
Antibody: anti-CTIP2 [25B6] (ab18465)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1 B1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: face mesenchyme Present Regionally restricted 1B1 , 1B3 Expression is in mesenchyme anterior to the premaxilla.
TS22: premaxilla Present Regionally restricted 1B3 , 1B1 Expression is in the osteogenic mesenchyme and is absent in the mature osteoblasts at the core.
TS22: nasal septum cartilage Absent 1B3 , 1B1 Including the turbinates.

Specimen 1 C1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: maxilla Present Regionally restricted 1C3 , 1C1 Expression is in the osteogenic mesenchyme at the premaxillary/maxillary suture and is absent in the mature osteoblasts at the core.
TS22: premaxilla Present Regionally restricted 1C3 , 1C1 Expression is in the osteogenic mesenchyme at the premaxillary/maxillary suture and is absent in the mature osteoblasts at the core.

Specimen 1 D1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: maxilla Present 1D1 , 1D3 Expression is in the osteogenic mesenchyme at the maxillary/palatine suture and is absent in the mature osteoblasts at the core.
TS22: palatal shelf Present 1D1 , 1D3 Expression is in the osteogenic mesenchyme at the maxillary/palatine suture and is absent in the mature osteoblasts at the core.

Specimen 1 E1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: face mesenchyme Present Regionally restricted 1E1 , 1E3 Expression is in the mesenchyme anterior to the mandible.

Specimen 1 F1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: inter-parietal bone primordium Present 1F3 , 1F1 Highest expression is at the periphery.

Specimen 2 B1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: nasal septum Present Regionally restricted 2B3 , 2B1 Expression is in the vomer bones of the nasal septum.
TS22: nasal septum cartilage Absent 2B3 , 2B1 Including the turbinates.

Specimen 2 C1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: maxilla Present Regionally restricted 2C3 , 2C1 Expression is in the lateral and medial peripheries of the maxillary bone.
TS22: palatal shelf mesenchyme Present Regionally restricted 2C1 , 2C3 Expression is in the condensing mesenchyme adjacent to the medial epithelial stream of the fusing palatal shelves.

Specimen 2 D1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: maxilla Present Regionally restricted 2D3 , 2D1 Expression is in the lateral and medial peripheries of the maxillary bone.
TS22: palatal shelf mesenchyme Present Regionally restricted 2D1 , 2D3 Expression is in the condensing mesenchyme adjacent to the medial epithelial stream of the fusing palatal shelves.

Specimen 2 E1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: palatal shelf mesenchyme Present Single cells 2E3 , 2E1 Expression is in a few cells adjacent to the medial palatal epithelium.
TS22: maxilla Present Regionally restricted 2E3 , 2E1 Expression is at the peripheries of the maxillary bone.

Specimen 2 F1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: viscerocranium Present Regionally restricted 2F3 , 2F1 Expression is in the nasal bone anlage.
TS22: nasal mesenchyme Present Regionally restricted 2F1 , 2F3 Expression is in the internasal suture mesenchyme.
TS22: nasal septum cartilage Absent 2F1 , 2F3 Including the turbinates.

Specimen 2 G1: embryonic day 14.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS22: frontal bone primordium Present Regionally restricted 2G1 , 2G3 Expression is at the periphery.

Specimen 3 B1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: face mesenchyme Present Regionally restricted 3B3 , 3B1 Expression is in the distal facial mesenchyme.

Specimen 3 C1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: viscerocranium Present Regionally restricted 3C1 , 3C3 Expression is in the nasal bone.
TS24: nose Present Regionally restricted 3C3 , 3C1 Expression is in the mesenchyme surrounding the nasal bone.
TS24: frontal bone Present 3C1 , 3C3
TS24: vault of skull Present Regionally restricted 3C1 , 3C3 Expression is in the mesenchyme of the nasopremaxillary suture, the frontonasal suture and the maxillary/palatine suture.
TS24: hard palate mesenchyme Present Regionally restricted 3C3 , 3C1 Expression is in the sutures and the mesenchyme surrounding the posterior edge of the palantine bone.

Specimen 3 D1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: maxilla Present Regionally restricted 3D3 , 3D1 Expression is a few cell layers thick along the bone surfaces.
TS24: premaxilla Present Regionally restricted 3D3 , 3D1 Expression is a few cell layers thick along the bone surfaces.
TS24: vault of skull Present Regionally restricted 3D3 , 3D1 Expression is in the maxillary/premaxillary suture.

Specimen 3 E1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: maxilla Present Regionally restricted 3E3 , 3E1 Expression is along the bone surfaces.
TS24: secondary palate Present Regionally restricted 3E3 , 3E1 Expression is along the bone surfaces.
TS24: vault of skull Strong Regionally restricted 3E3 , 3E1 Expression is in the maxillary/palatine suture.

Specimen 3 F1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: secondary palate Present Regionally restricted 3F1 , 3F3 Expression is along the bone surfaces.
TS24: vault of skull Present Regionally restricted 3F1 , 3F3 Expression is in the sutures.

Specimen 4 B1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: maxilla Present Regionally restricted 4B3 , 4B1 Expression is along the bone surfaces.
TS24: secondary palate Present Regionally restricted 4B3 , 4B1 Expression is along the bone surfaces.
TS24: vault of skull Strong Regionally restricted 4B1 , 4B3 Expression is in the maxillary/palatine surface.

Specimen 4 C1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: palate epithelium Strong 4C1 , 4C3
TS24: palate mesenchyme Present Regionally restricted 4C3 , 4C1 Expression is in the mesenchyme at the medial periphery and is weaker in the midline interpalatine suture.
TS24: vault of skull Present Regionally restricted 4C3 , 4C1 Expression is in the midline interpalatine suture.

Specimen 4 D1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: head mesenchyme Present Regionally restricted 4D3 , 4D1 Expression is in the mesenchyme surrounding the vertical extensions of the palatine bones.

Specimen 4 E1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: frontal bone Present Regionally restricted 4E3 , 4E1 Expression is along the outer margins.

Specimen 4 F1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: cranium Present Regionally restricted 4F3 , 4F1 Expression is in the suture mesenchyme between the zygoma and zygomatic process of the maxillary bone.
TS24: maxilla Present Regionally restricted 4F3 , 4F1 Expression is in the suture mesenchyme between the zygoma and zygomatic process of the maxillary bone.

Specimen 4 G1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: molar epithelium Present 4G3 , 4G1
TS24: jaw mesenchyme Present Regionally restricted 4G1 , 4G3 Expression is in the mesenchyme adjacent to the molar epithelium.

Specimen 5 A1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: frontal bone Present Regionally restricted 5A1 , 5A3 Expression is in the mesenchyme at the peripheries of the bone edges.
TS24: parietal bone Present Regionally restricted 5A1 , 5A3 Expression is in the mesenchyme at the peripheries of the bone edges.

Specimen 5 B1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: frontal bone Present Regionally restricted 5B3 , 5B1 Expression is in the mesenchyme at the peripheries of the bone edges.
TS24: parietal bone Present Regionally restricted 5B3 , 5B1 Expression is in the mesenchyme at the peripheries of the bone edges.

Specimen 5 C1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: vault of skull Present Regionally restricted 5C3 , 5C1 Expression is in the coronal suture mesenchyme.

Specimen 5 D1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS24: vault of skull Present 5D3 , 5D1 Expression is in the squamo-parietal suture.

Specimen 6 B1: embryonic day 18.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS26: suture Present Regionally restricted 6B1 , 6B3 Expression is in the nasopremaxillary suture.
TS26: premaxilla Present Regionally restricted 6B1 , 6B3 Expression is in 2-3-cell layer at the edge of the bone.

Specimen 6 C1: embryonic day 18.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS26: suture Present Regionally restricted 6C1 , 6C3 Expression is in the interpremaxillary suture.

Specimen 6 D1: embryonic day 18.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS26: suture Present Regionally restricted 6D3 , 6D1 Expression is in the intranasal suture.

Specimen 6 E1: embryonic day 18.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS26: coronal suture Present Regionally restricted 6E3 , 6E1 Expression is in the suture mesenchyme between the frontal and parietal bone.
TS26: frontal bone Present Regionally restricted 6E3 , 6E1 Expression is at the inner table edge of the frontal bone.
TS26: squamo-parietal suture Present 6E3 , 6E1

Specimen 6 F1: embryonic day 18.5 (more )
Note: Double labeled: red - Bcl11b; green Runx2 (assay MGI:5699546).
Structure Level Pattern Image Note
TS26: suture Weak Regionally restricted 6F3 , 6F1 Expression is at the interfrontal suture.
TS26: frontal bone Present Regionally restricted 6F3 , 6F1 Expression is at the peripheral edges.
TS26: inter-parietal bone Present Regionally restricted 6F3 , 6F1 Expression is at the peripheral edges.
TS26: parietal bone Present Regionally restricted 6F3 , 6F1 Expression is at the peripheral edges.

Specimen 8 A1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Satb2 (assay MGI:5699549).
Structure Level Pattern Image Note
TS24: vault of skull Present 8A1 , 8A4 Expression is in the inerpalatine suture.

Specimen 8 B1: embryonic day 16.5 (more )
Note: Double labeled: red - Bcl11b; green Satb2 (assay MGI:5699549).
Structure Level Pattern Image Note
TS24: vault of skull Present Regionally restricted 8B4 , 8B1 Expression is at the maxillary/palatine suture.

Specimen not shown E14.4: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: inner ear Absent

Specimen not shown E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: mandible Absent

Specimen Table 1 E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: vault of skull Present Regionally restricted Expression is in the frontonasal, nasopremaxillary, premaxillary/maxillary, maxillary/palatine, internasal, and coronal sutures.

Specimen Table 1 E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: vault of skull Present Regionally restricted Expression is in the frontonasal, nasopremaxillary, premaxillary/maxillary, maxillary/palatine, internasal, interpremaxillary, intermaxillary, interpalatine, maxillary/zygomatic, squamoparietal, interfrontal, sagittal, lambdoid, and coronal sutures.

Specimen Table 1 E18.5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: coronal suture Moderate
TS26: lambdoid suture Weak
TS26: sagittal suture Weak
TS26: vault of skull Present Regionally restricted Expression is in the frontonasal, nasopremaxillary, premaxillary/maxillary, maxillary/palatine, internasal, interpremaxillary, intermaxillary, interpalatine, maxillary/zygomatic, squamoparietal, and interfrontal sutures.


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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory