Probe:	Reelin probe6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	This data was summarized in Table 1. The authors mentioned that both autoradiographic and digoxigenin-labelled riboprobe (MGI:5698526) techniques were used, and gave consistent results. Digoxigenin-labelled riboprobe method was somewhat less sensitive; therefore illustrations are based on the autoradiographic procedure.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Very strong	Regionally restricted		Expressed in Cajal-Retzius cells in the superficial tiers, not other cortical neurons.
TS22: cortical plate	Absent
TS22: hippocampus	Very strong	Regionally restricted		Expressed in the hippocampal anlage, with very high expression in the marginal zone (Cajal-Retzius cells) and some weak expression in the intermediate zone.
TS22: main olfactory bulb	Strong
TS22: preoptic area	Strong
TS22: basal forebrain	Moderate	Regionally restricted		Moderate expression in the septal area and nucleus of stria terminalis. Low expression in striatum.
TS22: telencephalon septum	Moderate	Regionally restricted		Expressed in the nucleus of stria terminalis.
TS22: corpus striatum	Weak
TS22: deep cerebellar nucleus	Weak
TS22: lateral geniculate nucleus	Weak
TS22: retina	Moderate
TS22: tectum	Moderate
TS22: zona incerta	Moderate
TS22: habenula	Absent
TS22: midbrain tegmentum	Absent
TS22: thalamus	Absent
TS22: hypothalamus	Moderate	Regionally restricted		Expressed in hypothalamic nuclei.
TS22: cerebellum	Strong	Regionally restricted		High to moderate expression in external granule cell layer. No expression in Purkinje cells.
TS22: brainstem	Weak			Expressed in putative reticular neurons.
TS22: spinal cord lateral wall	Strong	Regionally restricted		Strong to moderate expression in anterior horn neurons.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex marginal zone	Very strong	Regionally restricted		Expressed in Cajal-Retzius cells, not other cortical neurons.
TS25: cerebral cortex intermediate zone	Absent
TS25: cerebral cortex ventricular zone	Absent
TS25: cortical plate	Absent
TS25: dentate gyrus	Very strong	Regionally restricted		Expressed in Cajal-Retzius neurons. Weak expression in the intermediate zone.
TS25: hippocampus	Very strong	Regionally restricted		Expressed in Cajal-Retzius neurons. Weak expression in the intermediate zone.
TS25: cerebellum external granule cell layer	Strong
TS25: main olfactory bulb	Strong
TS25: telencephalon septum	Moderate	Regionally restricted		Expressed in the septal area and the nucleus of stria terminalis.
TS25: corpus striatum	Moderate
TS25: habenula	Moderate
TS25: lateral geniculate nucleus	Moderate
TS25: retina	Moderate
TS25: tectum	Moderate
TS25: zona incerta	Moderate
TS25: deep cerebellar nucleus	Weak
TS25: midbrain tegmentum	Weak
TS25: preoptic area	Weak
TS25: reticular thalamic nucleus	Weak
TS25: thalamus	Weak
TS25: hypothalamus	Weak	Regionally restricted		Expressed in hypothalamic nuclei.
TS25: cerebellum layer	Strong	Regionally restricted		High to moderate expression in external granule cell layer. No expression in Purkinje cells.
TS25: ventral grey horn	Moderate			Anterior horn neurons.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong			High to moderate expression.
TS27: cerebellum internal granule cell layer	Strong			High to moderate expression.
TS27: main olfactory bulb	Strong			High to moderate expression.
TS27: cerebral cortex	Very strong	Regionally restricted		Expressed in Cajal-Retzius cells in the superficial tiers, not other cortical neurons.
TS27: dentate gyrus	Very strong	Regionally restricted		Expressed in the hippocampal anlage, with very high expression in the marginal zone (Cajal-Retzius cells) and some weak expression in the intermediate zone.
TS27: hippocampus	Very strong	Regionally restricted		Expressed in the hippocampal anlage, with very high expression in the marginal zone (Cajal-Retzius cells) and some weak expression in the intermediate zone.
TS27: bed nucleus of stria terminalis	Moderate
TS27: corpus striatum	Moderate
TS27: habenula	Moderate
TS27: lateral geniculate nucleus	Moderate
TS27: retina	Moderate
TS27: tectum	Moderate
TS27: telencephalon septum	Moderate
TS27: zona incerta	Moderate
TS27: brainstem reticular formation	Weak
TS27: deep cerebellar nucleus	Weak
TS27: hypothalamic nucleus	Weak
TS27: midbrain tegmentum	Weak
TS27: preoptic area	Weak
TS27: thalamus	Weak
TS27: cerebellum Purkinje cell layer	Absent
TS27: spinal cord ventral horn	Moderate			Anterior horn neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong			High to moderate expression.
TS28: cerebellum internal granule cell layer	Strong			High to moderate expression.
TS28: main olfactory bulb	Strong			High to moderate expression.
TS28: cerebral cortex	Very strong	Regionally restricted		Very strong expression in Cajal-Retzius cells. High to moderate expression in other cortical neurons.
TS28: dentate gyrus layer	Very strong	Regionally restricted		Expressed in Cajal-Retzius cells and some weak expression in the intermediate zone.
TS28: hippocampus layer	Very strong	Regionally restricted		Expressed in Cajal-Retzius cells and some weak expression in the intermediate zone.
TS28: bed nucleus of stria terminalis	Moderate
TS28: corpus striatum	Moderate
TS28: telencephalon septum	Moderate
TS28: brainstem reticular formation	Weak
TS28: deep cerebellar nucleus	Weak
TS28: habenula	Weak
TS28: hypothalamic nucleus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: midbrain tegmentum	Weak
TS28: preoptic area	Weak
TS28: tectum	Weak
TS28: thalamus	Weak
TS28: zona incerta	Weak
TS28: cerebellum Purkinje cell layer	Absent
TS28: spinal cord ventral horn	Weak			Anterior horn neurons.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong			High to moderate expression.
TS28: neocortex layer	Moderate			Expressed in in Cajal-Retzius cells and other cortical neurons.
TS28: dentate gyrus layer	Moderate			Expressed in Cajal-Retzius cells and the intermediate zone.
TS28: hippocampus layer	Moderate			Expressed in Cajal-Retzius cells and the intermediate zone.
TS28: bed nucleus of stria terminalis	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: corpus striatum	Absent
TS28: habenula	Absent
TS28: hypothalamic nucleus	Absent
TS28: lateral geniculate nucleus	Absent
TS28: midbrain tegmentum	Absent
TS28: telencephalon septum	Absent
TS28: thalamus	Absent
TS28: brainstem reticular formation	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: deep cerebellar nucleus	Weak
TS28: preoptic area	Weak
TS28: tectum	Weak
TS28: zona incerta	Weak