Probe:	Reelin probe6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The authors mentioned that both autoradiographic and digoxigenin-labelled riboprobe (MGI:5698526) techniques were used, and gave consistent results. Digoxigenin-labelled riboprobe method was somewhat less sensitive; therefore illustrations are based on the autoradiographic procedure.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Very strong	Regionally restricted	1A/A' , 1A inset	Expressed in the layer of developing mitral cells.

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Strong		1B/B'
TS22: neural retinal epithelium	Strong	Regionally restricted	1B/B'	Expressed in postmigratory neurons, probably ganglion cells.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Strong	Regionally restricted	1C/C'	Expressed in the hippocampal anlage, with heavy expression in the marginal zone and some weak expression in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Strong		1D	Expressed in Cajal-Retzius cells.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present		1E	Expressed in Cajal-Retzius cells.

Structure	Level	Pattern	Image	Note
TS22: basal forebrain	Present		2A/A'	Weaker expression in the external part, corresponding to the future pyriform cortex. Weak expression in striatum. Strong expression in the bed nucleus of the stria terminalis.
TS22: preoptic area	Strong		2A/A'
TS22: hypothalamus	Strong	Regionally restricted	2A/A'	Expressed in part of the hypothalamus.
TS22: forebrain ventricular layer	Absent		2A/A'

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Moderate	Regionally restricted	2B/B'	Expressed in septal nuclei.
TS22: preoptic area	Strong		2B/B'

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	3A/A'	Expressed in the external granular layer, and in some unidentified cells below the early Purkinje cell plate, possibly developing central nuclei. No expression in Purkinje cells.
TS22: hindbrain	Weak	Regionally restricted	3A/A'	Expressed in neuroepithelial cells in the germinal zone adjacent to the fourth ventricle. Some labelled cells were observed in tegmental fields, most probably associated with reticular nuclei.

Structure	Level	Pattern	Image	Note
TS22: tectum	Present	Regionally restricted	3B/B'	Expressed in the outer tier, particularly in the paramedian field (marginal zone). Tectal labelling was stronger at rostral levels. No expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Strong	Regionally restricted	3C/C'	Expressed in ventral horns. Weaker expression in the ventral horns.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Absent			Either at the future neocortical or the hippocampal levels.
TS22: thalamus	Present	Regionally restricted		Lower level expression in the thalamic fields.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong	Regionally restricted	4A	Expressed in Cajal-Retzius cells at the periphery.
TS25: neocortex	Strong	Regionally restricted	4A	Expressed in the cingulate cortex.
TS25: hippocampus	Weak	Regionally restricted	4A	Expressed in the developing straitum.
TS25: telencephalon septum	Weak	Regionally restricted	4A	Expressed in the lateral and medial septal nuclei.

Structure	Level	Pattern	Image	Note
TS25: neural retina	Strong	Regionally restricted	4B/B'	Expressed in the ganglion cell layer.
TS25: pigmented retinal epithelium	Absent		4B/B'

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Strong	Regionally restricted	4C/C'	Expressed in Cajal-Retzius neurons both in the developing Ammons horns and the incipient dentate gyrus.

Structure	Level	Pattern	Image	Note
TS25: lateral geniculate nucleus	Moderate		4D/D'
TS25: zona incerta	Moderate		4D/D'

Structure	Level	Pattern	Image	Note
TS25: lateral habenular nucleus	Present		5A/A'	More in the medial than in the lateral nucleus.
TS25: medial habenular nucleus	Present		5A/A'	More in the medial than in the lateral nucleus.
TS25: cerebral cortex	Strong	Regionally restricted	5A/A'	Expressed in Cajal-Retzius cells at the periphery.
TS25: hippocampus	Strong	Regionally restricted	5A/A'	Expressed in Cajal-Retzius neurons in the developing Ammons horns.

Structure	Level	Pattern	Image	Note
TS25: cerebellum granule cell layer	Present	Regionally restricted	5B/B'	Expressed in the external granular cells and migrating granular neurons.
TS25: cerebellum external granule cell layer	Strong		5B/B'
TS25: cerebellum layer	Present	Regionally restricted	5B/B'	Expressed in the external granular layer. No expression in Purkinje cells. Weak expression in the cerebellar nuclei.

Structure	Level	Pattern	Image	Note
TS25: cerebellum granule cell layer	Present	Regionally restricted	5C	Expressed in the external granular cells and migrating granular neurons.
TS25: cerebellum external granule cell layer	Present		5C

Structure	Level	Pattern	Image	Note
TS25: inferior colliculus	Weak	Regionally restricted	5D/D'	Expressed in the marginal layer. Expression was maximal in the paramedian region. No expression in the ventricular zones.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Absent
TS25: cerebral cortex ventricular zone	Absent
TS25: cortical plate	Absent
TS25: main olfactory bulb	Very strong			Expressed in mitral cells and tufted cells.
TS25: hypothalamus	Moderate
TS25: midbrain tegmentum	Present			Expressed in the future reticulotegmental nucleus.
TS25: pons	Weak	Regionally restricted		Expressed in the ventral field. No expression in the inferior olivary complex and facial nerve nuclei.
TS25: brainstem	Weak	Regionally restricted		Expressed in the medullary reticular formation.
TS25: ventral grey horn	Strong			Anterior horn neurons.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Strong		6A/A'
TS27: main olfactory bulb granule cell layer	Absent		6A/A'	Barely detectable.
TS27: neocortex	Strong	Regionally restricted	6A/A'	Expressed in Cajal-Retzius cells of the marginal zone.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Strong	Regionally restricted	6B	Expressed in Cajal-Retzius cells of the marginal zone.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Strong		6C
TS27: main olfactory bulb granule cell layer	Absent		6C

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Strong		6D/D'

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Strong	Regionally restricted	7A/A'	Expressed in Cajal-Retzius neurons in the marginal zone.
TS27: hippocampus	Strong	Regionally restricted	7A/A'	Expressed in Cajal-Retzius neurons in the marginal zone.

Structure	Level	Pattern	Image	Note
TS27: paraventricular hypothalamic nucleus	Weak		7B/B'

Structure	Level	Pattern	Image	Note
TS27: accumbens nucleus	Moderate			Moderate to low expression.
TS27: piriform cortex	Moderate
TS27: olfactory tubercle	Moderate	Regionally restricted		Expressed in Calleja islands.
TS27: habenula	Present
TS27: lateral geniculate nucleus	Present
TS27: zona incerta	Present
TS27: tectum	Present	Regionally restricted		Expressed in the superficial tiers, more concentrated in the midline than in the lateral field and appeared weaker than at the previous embryonic stage.
TS27: cochlear nucleus	Present
TS27: midbrain tegmentum	Present
TS27: posterior commissure	Present
TS27: cerebellum external granule cell layer	Present	Regionally restricted		Expressed in the inner tiers.
TS27: hindbrain	Moderate	Scattered		Associated with parts of the reticular formation.
TS27: brainstem reticular formation	Present	Regionally restricted		Expressed dorsally to the inferior olive.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Strong	Regionally restricted	8A inset , 8A/A'	Expressed in Cajal-Retzius cells in the marginal zone. Also expressed in scattered neurons in the cortical plate, more in its inner tiers than in the outer tiers.
TS28: neocortex layer	Strong	Scattered	8A inset , 8A/A'	Positive cortical cells were not very abundant and distributed throughout all cortical layers, although more concentrated in the inner part of the cortex, at the level of future laminae V and VI.
TS28: cortical layer V	Strong	Scattered	8A inset , 8A/A'
TS28: cortical layer VI	Strong	Scattered	8A inset , 8A/A'

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Weak		8B/B'

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		9A/A'
TS28: cerebellum internal granule cell layer	Present		9A/A'
TS28: cerebellum Purkinje cell layer	Absent		9A/A'
TS28: deep cerebellar nucleus	Trace		9A/A'	Very low expression in the neurons.

Structure	Level	Pattern	Image	Note
TS28: brainstem reticular formation	Moderate	Regionally restricted	9B/B' , 9C	Expressed in a stream of neurons, presumably a component of the reticular formation.
TS28: inferior olivary nucleus	Absent		9B/B'

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		9D
TS28: cerebellum internal granule cell layer	Present		9D

Structure	Level	Pattern	Image	Note
TS28: hippocampus layer	Present	Regionally restricted		Expressed in Cajal-Retzius cells and the infrapyramidal layer.
TS28: dentate gyrus layer	Present	Regionally restricted		Expressed in Cajal-Retzius cells and the hilar region.
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: main olfactory bulb glomerular layer	Very strong			Very strong expression in tufted cells. Moderate expression in the layer, probably in periglomerular neurons.
TS28: brain subventricular zone	Weak			Associated with oblique or tangentially orientated cells.
TS28: bed nucleus of stria terminalis	Weak
TS28: reticular tegmental nucleus	Weak
TS28: septal nucleus	Weak
TS28: basal ventral medial thalamic nucleus	Present
TS28: habenula	Present
TS28: preoptic area	Present
TS28: superior colliculus	Present
TS28: trigeminal V motor nucleus	Present
TS28: zona incerta	Present
TS28: thalamic nucleus	Weak			Weak expression, with the exception of the lateral geniculate nucleus that was moderately positive.
TS28: lateral geniculate nucleus	Moderate

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present	Scattered	10A/A'	Expressed throughout all layers, without any systematic organization.
TS28: cortical layer I	Present	Scattered	10A/A'	Fewer labelled Cajal-Retzius cells than during development.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present	Scattered	10B

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present	Scattered	10C

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Very strong		11A/A'
TS28: main olfactory bulb glomerular layer	Present	Regionally restricted	11A/A'	Expressed in the most internal tiers. Weaker expression in the periglomemlar cells.
TS28: main olfactory bulb external plexiform layer	Absent		11A/A'
TS28: main olfactory bulb granule cell layer	Absent		11A/A'
TS28: main olfactory bulb internal plexiform layer	Absent		11A/A'

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present	Diffuse	11B/B'	There is some weak labelling as well as some scattered neurons with a more intense signal, particularly in the cortical contingent.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Moderate			Associated with tangentially orientated cells.
TS28: entorhinal cortex	Strong	Diffuse
TS28: basal ventral medial thalamic nucleus	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum external granule cell layer	Absent
TS28: telencephalon septum	Absent
TS28: brainstem reticular formation	Present
TS28: preoptic area	Present
TS28: superior colliculus	Present
TS28: zona incerta	Present
TS28: cerebellum internal granule cell layer	Weak