About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:211182 Deng M, et al., Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2014 May;15(1):31-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5697719
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Antibody: anti-Brn-3a (14A6; sc-8429)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A/C: embryonic day 9.5 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Strong Regionally restricted 1C , 1A Expressed in the majority of the delaminated cochleovestibular ganglion neurons and a few of facial ganglion cells.
TS15: otic pit epithelium Absent 1C , 1A

Specimen 1D/F: embryonic day 10.5 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present Regionally restricted 1F , 1D Increased expression. Expressed in the majority of the delaminated cochleovestibular ganglion neurons.
TS17: otocyst epithelium Absent 1F , 1D

Specimen 1G/I: embryonic day 11.5 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 1I , 1G Increased expression. Expressed in the majority of the delaminated cochleovestibular ganglion neurons.
TS19: inner ear epithelium Absent 1G , 1I

Specimen 1J/L: embryonic day 12.5 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS20: vestibulo-cochlear ganglion Present 1J , 1L Expressed in nearly all cells.
TS20: cochlear ganglion Present 1J , 1L Peak expression in nearly all cells. Co-localized with Pou4f2 positive cells.

Specimen 1M/O: embryonic day 15.5 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS23: cochlear ganglion Present 1O , 1M Peak expression in nearly all cells. Co-localized with Pou4f2 positive cells.

Specimen 1P/R: embryonic day 17.5 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS25: cochlear ganglion Present 1R , 1P Expressing cells were gradually reduced.

Specimen 1S/U: postnatal day 0 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS27: cochlear ganglion Present 1S , 1U Reduced expression.

Specimen 1V/X: postnatal day 0 (more )
Note: Double labeled: red - Pou4f1; green - Pou4f2 (assay MGI:5697723).
Structure Level Pattern Image Note
TS27: vestibular ganglion Present 1X , 1V Co-localized with Pou4f2 positive cells.

Specimen 2A/C: embryonic day 9.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Strong Regionally restricted 2A , 2C Expressed in the majority of the delaminated cochleovestibular ganglion neurons and a few of facial ganglion cells.
TS15: otic pit epithelium Absent 2C , 2A

Specimen 2D/F: embryonic day 10.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present Regionally restricted 2D , 2F Expressed in the majority of the delaminated cochleovestibular ganglion neurons.
TS17: otocyst epithelium Absent 2F , 2D

Specimen 2G/I: embryonic day 11.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 2G , 2I Expressed in the majority of the delaminated cochleovestibular ganglion neurons.

Specimen 2J/L: embryonic day 12.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS20: vestibulo-cochlear ganglion Present 2J , 2L Expressed in the majority of the delaminated cochleovestibular ganglion neurons.

Specimen 2M/O: embryonic day 13.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS21: cochlear ganglion Present Widespread 2M , 2O Coexpressed with Isl1.
TS21: vestibular ganglion Present Widespread 2M , 2O Coexpressed with Isl1.
TS21: vestibulo-cochlear ganglion Present Widespread 2M , 2O Expression level was higher in the spiral ganglion than in the vestibular ganglion.

Specimen 2P/R: embryonic day 15.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS23: cochlear ganglion Strong 2R , 2P

Specimen 2S/U: embryonic day 17.5 (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:5697725).
Structure Level Pattern Image Note
TS25: cochlear ganglion Present 2S , 2U Down-regulated expression.

Specimen 3G/I: embryonic day 10.5 (more )
Note: Double labeled: red - Pou4f1; green - Mki67 (assay MGI:5697840).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 3I , 3G Expressed in proliferating cells as well as in post-mitotic cells in the cochleovestibular ganglion.
TS17: facial ganglion Present 3I , 3G


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
05/07/2024
MGI 6.23
The Jackson Laboratory