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Gene Expression Data
Assay Details
Assay
Reference: J:211182 Deng M, et al., Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2014 May;15(1):31-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5697723
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Antibody: anti-Pou4f2 (C-13) sc-6026
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1B/C: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present Single cells 1C , 1B Expressed in only a very few cochleovestibular ganglion cells, which coexpressed Pou4f1.
TS15: otic pit epithelium Absent 1C , 1B
Specimen 1E/F: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 1E , 1F Increased expression. Expression started in the kernel of cochleovestibular ganglion cells and co-localized with Pou4f1 positive cells.
TS17: otocyst epithelium Absent 1F , 1E
Specimen 1H/I: embryonic day 11.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 1I , 1H Increased expression. Expression started in the kernel of cochleovestibular ganglion cells and co-localized with Pou4f1 positive cells.
TS19: inner ear epithelium Absent 1H , 1I
Specimen 1K/L: embryonic day 12.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS20: cochlear ganglion Present Regionally restricted 1K , 1L Expressed in a small group of cells near the cochlear duct.
TS20: vestibulo-cochlear ganglion Present Regionally restricted 1L , 1K Expressed in the center.
Specimen 1N/O: embryonic day 15.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS23: cochlear ganglion Present Widespread 1N , 1O Expression expanded to almost all spiral ganglion neurons, co-localizing with the Pou4f1-expressing cells.
Specimen 1Q/R: embryonic day 17.5 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS25: cochlear ganglion Strong 1R , 1Q
Specimen 1T/U: postnatal day 0 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS27: cochlear ganglion Strong 1T , 1U
Specimen 1W/X: postnatal day 0 (more )
Note: Double labeled: green - Pou4f2; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS27: vestibular ganglion Strong 1W , 1X
Specimen 4A/C: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f2; red - Neurod1 (assay MGI:5697839).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Present Single cells 4C , 4A Only expressed in Neurod1-negative, differentiated neural cochleovestibular ganglion cells.
Specimen 4D/F: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f2; red - Neurod1 (assay MGI:5697839).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present Regionally restricted 4D , 4F Only expressed in Neurod1-negative, differentiated neural cochleovestibular ganglion cells.
Specimen 4G/I: embryonic day 11.5 (more )
Note: Double labeled: green - Pou4f2; red - Neurod1 (assay MGI:5697839).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 4G , 4I Only expressed in Neurod1-negative, differentiated neural cochleovestibular ganglion cells.
Specimen 4M/O: embryonic day 11.5 (more )
Note: Double labeled: green - Pou4f2; red - BrdU.
Structure Level Pattern Image Note
TS19: acoustic ganglion Present Regionally restricted 4O , 4M Not expressed in proliferating cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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