Antibody:	VEGF-B186 antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: diaphragm	Strong		5B
TS20: heart	Strong		5B
TS20: skeletal muscle	Strong		5B

Structure	Level	Pattern	Image	Note
TS17: heart atrium	Very strong		6B
TS17: primitive ventricle	Very strong		6B
TS17: endocardial cushion	Absent		6B
TS17: pericardium	Absent		6B
TS17: heart septum	Absent		6B	Absent in the atrioventricular septum.

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Very strong		6C
TS20: ventricle cardiac muscle	Very strong		6C
TS20: outflow tract	Very strong	Regionally restricted	6C	Expression is proximal to the heart and is absent distally and in endothelial cells.
TS20: atrioventricular endocardial cushion	Absent		6C

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Strong		6D	Staining is perinuclear with some staining in the extracellular matrix.
TS20: endocardial lining	Absent		6D
TS20: pericardium	Absent		6D

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Weak	Regionally restricted	6E	Expression is in scattered smooth muscle cells.
TS20: blood vessel endothelium	Absent		6E

Structure	Level	Pattern	Image	Note
TS23: carotid artery	Present	Regionally restricted	6F	Expression is in smooth muscle.
TS23: blood vessel endothelium	Absent		6F

Structure	Level	Pattern	Image	Note
TS22: lung vascular element	Present	Regionally restricted	6G	Expression is in smooth muscle.
TS22: blood vessel endothelium	Absent		6G

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present		6H
TS23: arch of aorta	Absent		6H	Absent in smooth muscle cells.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		7A

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Present		7B
TS22: ventricle cardiac muscle	Present		7B
TS22: skeletal muscle	Present	Regionally restricted	7B	Expression is in thoracic muscle.
TS22: mesothelium	Absent		7B

Structure	Level	Pattern	Image	Note
TS23: diaphragm	Strong		7C

Structure	Level	Pattern	Image	Note
TS22: bone	Present	Regionally restricted	7D	Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS23: rib	Present	Regionally restricted	7E	Expression is in the perichondrium.
TS23: intercostal muscle	Present		7E

Structure	Level	Pattern	Image	Note
TS23: Meckel's cartilage	Present	Regionally restricted	7F	Expression is in chondrocytes.

Structure	Level	Pattern	Image	Note
TS25: adrenal cortex	Present		7G
TS25: skeletal muscle	Present		7G

Structure	Level	Pattern	Image	Note
TS25: pancreas parenchyma	Present		7H

Structure	Level	Pattern	Image	Note
TS17: atrium cardiac muscle	Strong
TS17: primitive ventricle cardiac muscle	Strong
TS17: myotome	Strong	Regionally restricted		Expression is in the intersegmental muscle of the vertebrate column.
TS17: dorsal aorta	Absent
TS17: lung vascular element	Absent
TS17: pericardium	Absent
TS17: vertebral cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Strong
TS19: ventricle cardiac muscle	Strong
TS19: myotome	Strong	Regionally restricted		Expression is in intersegmental muscle of the vertebrate column.
TS19: basilar artery	Absent
TS19: carotid artery	Absent
TS19: dorsal aorta	Absent
TS19: hyoid cartilage primordium	Absent
TS19: lung vascular element	Absent
TS19: pericardium	Absent
TS19: rib pre-cartilage condensation	Absent
TS19: vertebral cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Strong
TS20: choroid plexus	Strong
TS20: ventricle cardiac muscle	Strong
TS20: venous system	Strong	Regionally restricted		Expression is in the smooth muscle layer of the pulmonary vein.
TS20: skeletal muscle	Strong	Regionally restricted		Expression is in intersegmental muscle of the vertebrate column and weaker expression in the external eye muscle and facial muscle.
TS20: Meckel's cartilage	Present
TS20: diaphragm	Present
TS20: hyoid cartilage primordium	Present
TS20: tongue muscle	Present
TS20: basilar artery	Present	Regionally restricted		Expression is in the smooth muscle layer.
TS20: carotid artery	Present	Regionally restricted		Expression is in the smooth muscle layer.
TS20: subclavian artery	Present	Regionally restricted		Expression is in the smooth muscle layer.
TS20: dorsal aorta	Trace	Single cells		Expression is in the smooth muscle layer.
TS20: pancreas	Trace	Single cells
TS20: lung vascular element	Absent
TS20: pericardium	Absent
TS20: rib pre-cartilage condensation	Absent
TS20: vertebral cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Strong
TS22: adrenal gland	Strong
TS22: atrium cardiac muscle	Strong
TS22: choroid plexus	Strong
TS22: intercostal muscle	Strong
TS22: pectoral girdle and thoracic body wall muscle	Strong
TS22: tongue muscle	Strong
TS22: tooth	Strong
TS22: ventricle cardiac muscle	Strong
TS22: basilar artery	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS22: lung vascular element	Strong	Regionally restricted		Expression is in the smooth muscle layer of lung arteries.
TS22: venous system	Strong	Regionally restricted		Expression is in the smooth muscle layer of the pulmonary vein.
TS22: skeletal muscle	Strong	Regionally restricted		Expression is in subcutaneous muscle, thoracic muscle, facial muscle, in the external eye muscle, and in intersegmental muscle of the vertebrate column.
TS22: bone	Strong	Regionally restricted		Expression is in the perichondrium of the long bones.
TS22: diaphragm	Present
TS22: hyoid cartilage primordium	Present
TS22: pericardium	Present
TS22: carotid artery	Present	Regionally restricted		Expression is in the smooth muscle layer.
TS22: subclavian artery	Present	Regionally restricted		Expression is in the smooth muscle layer.
TS22: rib cartilage condensation	Present	Regionally restricted		Expression is in the perichondrium.
TS22: arterial system	Present	Regionally restricted		Expression is in the smooth muscle layer of the arteries in the kidney.
TS22: dorsal aorta	Trace	Single cells		Expression is in the smooth muscle layer.
TS22: pancreas	Trace	Single cells
TS22: endocardial tissue	Absent
TS22: vertebral cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS23: Meckel's cartilage	Strong
TS23: atrium cardiac muscle	Strong
TS23: diaphragm	Strong
TS23: intercostal muscle	Strong
TS23: pectoral girdle and thoracic body wall musculature	Strong
TS23: pericardium	Strong
TS23: tongue muscle	Strong
TS23: tooth	Strong
TS23: ventricle cardiac muscle	Strong
TS23: basilar artery	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS23: carotid artery	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS23: pulmonary vein	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS23: subclavian artery	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS23: lung vascular element	Strong	Regionally restricted		Expression is in the smooth muscle layer of lung arteries.
TS23: skeletal muscle	Strong	Regionally restricted		Expression is in subcutaneous muscle, external eye muscle, hair follicle muscle, facial muscle, and in intersegmental muscle of the vertebrate column.
TS23: rib	Strong	Regionally restricted		Expression is in the perichondrium.
TS23: vertebra	Strong	Regionally restricted		Expression is in the perichondrium.
TS23: bone	Present	Regionally restricted		Expression is in the perichondrium of the long bones.
TS23: choroid plexus	Present
TS23: hyoid	Present
TS23: descending aorta	Trace	Single cells		Expression is in the smooth muscle layer.
TS23: pancreas	Trace	Single cells
TS23: adrenal gland	Absent
TS23: endocardial tissue	Absent

Structure	Level	Pattern	Image	Note
TS25: Meckel's cartilage	Strong
TS25: atrium cardiac muscle	Strong
TS25: brown fat	Strong
TS25: diaphragm	Strong
TS25: intercostal muscle	Strong
TS25: pectoral girdle and thoracic body wall musculature	Strong
TS25: pericardium	Strong
TS25: tongue muscle	Strong
TS25: ventricle cardiac muscle	Strong
TS25: lung vascular element	Strong	Regionally restricted		Expression is in the smooth muscle layer of lung arteries.
TS25: pulmonary vein	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS25: arterial system	Strong	Regionally restricted		Expression is in the smooth muscle layer of brown fat arteries and arteries in the intestine.
TS25: renal vasculature	Strong	Regionally restricted		Expression is in the smooth muscle layer of kidney arteries.
TS25: skeletal muscle	Strong	Regionally restricted		Expression is in subcutaneous muscle, external eye muscle, hair follicle muscle, facial muscle, and in intersegmental muscle of the vertebrate column.
TS25: basilar artery	Present	Regionally restricted		Expression is in the smooth muscle layer.
TS25: rib	Present	Regionally restricted		Expression is in the perichondrium.
TS25: bone	Present	Regionally restricted		Expression is in the perichondrium of the long bones.
TS25: adrenal gland	Present
TS25: choroid plexus	Present
TS25: pancreas	Present
TS25: tooth	Present
TS25: vertebra	Present
TS25: descending aorta	Trace	Single cells		Expression is in the smooth muscle layer.
TS25: endocardial tissue	Absent
TS25: hyoid	Absent