Probe:	VEGF-B cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present		3A/B

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		3C/D
TS13: amnion	Present		3C/D
TS13: yolk sac mesenchyme	Weak	Scattered	3C/D
TS28: placenta	Present	Single cells	3C/D	Expression is in some cells surrounding the venous lacunae.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		3E/F
TS28: placenta	Present	Single cells	3E/F	Expression is in some cells surrounding the venous lacunae.
TS13: yolk sac	Weak	Scattered	3E/F
TS13: ectoplacental cone	Absent		3E/F

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		4A/B
TS17: endocardial cushion	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Absent		4C/D

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present		4E/F
TS20: hindbrain roof	Present		4E/F

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	4G/H	Expression is in the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS22: heart	Strong		4I/J
TS22: intercostal muscle	Present		4I/J

Structure	Level	Pattern	Image	Note
TS20: diaphragm	Strong		5A
TS20: heart	Strong		5A
TS20: nasopharynx	Strong		5A
TS20: trunk mesenchyme	Strong	Regionally restricted	5A	Expression is in the mesenchyme surrounding the developing vetrebrae and the tissue surrounding the developing gastrointetsinal tract.
TS20: trunk	Strong	Regionally restricted	5A	Expression is in the lower abdominal wall close to the area of the genital tubercle.
TS20: lung	Weak		5A
TS20: metanephros	Weak		5A

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Present
TS13: primitive ventricle cardiac muscle	Present
TS13: somite	Present	Regionally restricted		Expression is in the myotome.

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Present
TS15: myotome	Present
TS15: primitive ventricle cardiac muscle	Present

Structure	Level	Pattern	Image	Note
TS17: atrium cardiac muscle	Strong
TS17: primitive ventricle cardiac muscle	Strong
TS17: myotome	Strong			Strong expression in myotomes and intersegmental muscle of the vertebrate column.

Structure	Level	Pattern	Image	Note
TS19: atrium cardiac muscle	Strong
TS19: ventricle cardiac muscle	Strong
TS19: myotome	Strong	Regionally restricted		Expression is in intersegmental muscle of the vertebrate column.

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Strong
TS20: choroid plexus	Strong
TS20: ventricle cardiac muscle	Strong
TS20: venous system	Strong	Regionally restricted		Expression is in the smooth muscle layer of the pulmonary vein.
TS20: skeletal muscle	Strong	Regionally restricted		Expression is in intersegmental muscle of the vertebrate column.
TS20: diaphragm	Present
TS20: tongue muscle	Present
TS20: pancreas	Trace	Single cells

Structure	Level	Pattern	Image	Note
TS22: atrium cardiac muscle	Strong
TS22: choroid plexus	Strong
TS22: intercostal muscle	Strong
TS22: pectoral girdle and thoracic body wall muscle	Strong
TS22: tongue muscle	Strong
TS22: ventricle cardiac muscle	Strong
TS22: lung vascular element	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS22: venous system	Strong	Regionally restricted		Expression is in the smooth muscle layer of the pulmonary vein.
TS22: skeletal muscle	Strong	Regionally restricted		Expression is in subcutaneous muscle, thoracic muscle, facial muscle, and in intersegmental muscle of the vertebrate column.
TS22: bone	Strong	Regionally restricted		Expression is in the perichondrium of the long bones.
TS22: diaphragm	Present
TS22: pancreas	Present
TS22: pericardium	Present
TS22: rib cartilage condensation	Present	Regionally restricted		Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS23: atrium cardiac muscle	Strong
TS23: diaphragm	Strong
TS23: intercostal muscle	Strong
TS23: pectoral girdle and thoracic body wall muscle	Strong
TS23: tongue muscle	Strong
TS23: ventricle cardiac muscle	Strong
TS23: lung vascular element	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS23: pulmonary vein	Strong	Regionally restricted		Expression is in the smooth muscle layer.
TS23: skeletal muscle	Strong	Regionally restricted		Expression is in subcutaneous muscle, thoracic muscle, facial muscle, and in intersegmental muscle of the vertebrate column.
TS23: rib	Strong	Regionally restricted		Expression is in the perichondrium.
TS23: vertebra	Strong	Regionally restricted		Expression is in the perichondrium.
TS23: choroid plexus	Present
TS23: pancreas	Present
TS23: pericardium	Present
TS23: bone	Present	Regionally restricted		Expression is in the perichondrium of the long bones.