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Gene Expression Data
Assay Details
Assay
Reference: J:203006 Zhuang S, et al., Expression of Isl1 during mouse development. Gene Expr Patterns. 2013 Dec;13(8):407-12
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5695112
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Reporter: lacZ
Antibody: anti-B-galactosidase (55976)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4A: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present 4A right , 4A merge Expressed in a manner completely overlapping with lacZ expression.
Specimen 4B: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted 4B right , 4B merge Expressed in the basal forebrain. The migrating neurons just leaving the subventricular zone were clearly labeled.
TS19: diencephalon lateral wall mantle layer Present 4B merge , 4B right Expression largely overlapped with Isl1 staining in the outer mantle layer where differentiated neurons reside.
Specimen 4C: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: diencephalon Present 4C right , 4C merge The migrating neurons just leaving the subventricular zone were clearly labeled.
Specimen 4D: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 4D merge , 4D right Expressed in the ventral and dorsal-lateral motor neurons.
Specimen 4E: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: trigeminal ganglion Present 4E
Specimen 4F: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: acoustic ganglion Present 4F merge , 4F right
TS19: facial ganglion Present 4F merge , 4F right
Specimen 4G: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: glossopharyngeal ganglion Present 4G merge , 4G right
TS19: vagus ganglion Present 4G merge , 4G right
Specimen 4H: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: branchial arch endoderm Present 4H right , 4H merge
TS19: branchial arch mesenchyme Present 4H right , 4H merge
TS19: carotid artery Present 4H right , 4H merge
TS19: vagus ganglion Present 4H right , 4H merge
Specimen 4I: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present 4I
TS19: future spinal cord Present Regionally restricted 4I Expressed in spinal motor neurons.
Specimen 4J: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: arytenoid swelling pre-cartilage condensation Present 4J
TS19: branchial arch endoderm Present 4J
Specimen 4K: embryonic day 11.5; Isl1tm3Sev/Isl1+ (more )
Note: Double labeled: red - Isl1 reporter; green - Isl1 protein (assay MGI:5695113).
Structure Level Pattern Image Note
TS19: outflow tract Present 4K merge , 4K right Expressed in myocardial cells of the distal and proximal outflow tract, endocardium and cushion mesenchyme.
TS19: outflow tract cardiac muscle Present 4K merge , 4K right
TS19: outflow tract distal part Present 4K merge , 4K right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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