Gene Expression Data
Assay Details
Assay
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Reference:
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J:84432
Qi Y, et al., Gli2 is required for normal Shh signaling and oligodendrocyte development in the spinal cord. Mol Cell Neurosci. 2003 Jul;23(3):440-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5694635
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Gene symbol:
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Mbp
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Gene name:
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myelin basic protein
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Probe:
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Mbp probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5A:
embryonic day 16.5
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
Regionally restricted |
5A
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Expression was detected in a small number of oligodendrocytes in the ventral spinal cord.
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Specimen
5B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5C:
embryonic day 18.5
(more )
Note: Section from the thoracic region.
Specimen
5C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section from the thoracic region.
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Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Regionally restricted |
5C
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Expression was detected in oligodendrocytes.
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Specimen
5D:
embryonic day 18.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: Section from the thoracic region.
Specimen
5D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Section from the thoracic region.
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Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Regionally restricted |
5D
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Expression was detected in a few oligodendrocytes, but much fewer than in wild type.
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Specimen
5E:
embryonic day 18.5
(more )
Note: Section from the cervical region.
Specimen
5E:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Section from the cervical region.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Regionally restricted |
5E
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Expression was detected in oligodendrocytes.
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Specimen
5F:
embryonic day 18.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: Section from the cervical region.
Specimen
5F:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Section from the cervical region.
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Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Regionally restricted |
5F
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In the cervical spinal cord of the mutants, expression was detected in many more oligodendrocytes than at the thoracic level of the mutants.
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