Unc5b RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199400 van den Heuvel DM, et al., Spatiotemporal expression of repulsive guidance molecules (RGMs) and their receptor neogenin in the mouse brain. PLoS One. 2013;8(2):e55828
Assay type:	RNA in situ
MGI Accession ID:	MGI:5693258
Gene symbol:	Unc5b
Gene name:	unc-5 netrin receptor B

Probe:	Unc5b cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2B ©

Specimen 2B: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: main olfactory bulb mitral cell layer	Present	2B
TS24: blood vessel	Present	2B
TS24: olfactory epithelium	Present	2B

2F ©

Specimen 2F: postnatal day 5 (more

)

Structure	Level	Image	Note
TS28: main olfactory bulb glomerular layer	Present	2F	Expression was reduced compared to E16.5.
TS28: main olfactory bulb granule cell layer	Present	2F	Expression was reduced compared to E16.5.
TS28: main olfactory bulb mitral cell layer	Present	2F	Expression was reduced compared to E16.5.

Specimen Table S2 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb mitral cell layer	Very strong
TS24: olfactory epithelium	Very strong
TS24: ethmoid bone cribriform plate	Absent
TS24: main olfactory bulb ventricular layer	Absent
TS24: accessory olfactory bulb	Strong
TS24: main olfactory bulb internal plexiform layer	Moderate
TS24: main olfactory bulb prospective granule cell layer	Moderate
TS24: main olfactory bulb external plexiform layer	Weak
TS24: main olfactory bulb prospective glomerular layer	Weak

Specimen Table S2 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: anterior olfactory nucleus	Absent
TS28: ethmoid bone cribriform plate	Absent
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: main olfactory bulb ventricular layer	Absent
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: olfactory epithelium	Strong

Specimen Table S2 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate
TS28: anterior olfactory nucleus	Absent
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: main olfactory bulb granule cell layer	Weak

5B ©

Specimen 5B: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebral cortex subventricular zone	Present	5B
TS24: cerebral cortex ventricular layer	Present	5B
TS24: cortical plate	Present	5B

5F ©

Specimen 5F: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Absent		5F

Specimen Table S3 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Moderate
TS24: cerebral cortex ventricular layer	Moderate
TS24: telencephalon pia mater	Moderate
TS24: cerebral cortex intermediate zone	Absent
TS24: cerebral cortex marginal layer	Absent
TS24: cortical subplate	Absent
TS24: cortical plate	Weak

Specimen Table S3 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex ventricular zone	Absent
TS28: cortical layer I	Absent
TS28: cortical layer II	Absent
TS28: cortical layer III	Absent
TS28: cortical layer IV	Absent
TS28: cortical layer V	Absent
TS28: cortical layer VI	Absent

Specimen Table S3 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex ventricular zone	Absent
TS28: cortical layer I	Absent
TS28: cortical layer II	Absent
TS28: cortical layer III	Absent
TS28: cortical layer IV	Absent
TS28: cortical layer V	Absent
TS28: cortical layer VI	Absent

7B ©

Specimen 7B: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	7B	Expression was detected in the CA region.
TS24: dentate gyrus primordium	Present		7B

7F ©

Specimen 7F: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Absent		7F

Specimen Table S4 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: dentate gyrus primordium	Moderate
TS24: hippocampus ventricular layer	Moderate
TS24: hippocampus	Weak	Regionally restricted	Strong expression was detected in the CA region, and moderate expression was detected in the subventricular zone.
TS24: entorhinal cortex	Weak

Specimen Table S4 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Absent
TS28: dentate gyrus granule cell layer	Absent
TS28: dentate gyrus molecular layer	Absent
TS28: dentate gyrus polymorphic layer	Absent
TS28: entorhinal cortex	Absent
TS28: hippocampus CA1	Absent
TS28: hippocampus CA2	Absent
TS28: hippocampus CA3	Absent
TS28: hippocampus stratum lacunosum	Absent
TS28: hippocampus stratum oriens	Absent
TS28: hippocampus stratum radiatum	Absent

Specimen Table S4 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Weak
TS28: hippocampus CA2	Weak
TS28: hippocampus CA3	Weak
TS28: dentate gyrus	Weak
TS28: dentate gyrus granule cell layer	Weak
TS28: dentate gyrus molecular layer	Absent
TS28: dentate gyrus polymorphic layer	Absent
TS28: entorhinal cortex	Absent
TS28: hippocampus stratum lacunosum	Absent
TS28: hippocampus stratum oriens	Absent
TS28: hippocampus stratum radiatum	Absent

10B ©

Specimen 10B: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: habenula	Weak	Regionally restricted	10B	Expression was detected in blood vessels, but not habenular neurons.
TS24: blood vessel	Present		10B

10F ©

Specimen 10F: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: habenula	Absent		10F

10J ©

Specimen 10J: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: habenula	Weak		10J

Specimen Table S5 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: midbrain	Absent		There was no expression in the substantia nigra and ventral tegmental area.
TS24: thalamus	Absent
TS24: corpus striatum	Moderate
TS24: telencephalon septum	Moderate
TS24: hypothalamus	Weak	Regionally restricted	Expression was weak in the lateral hypothalamus.
TS24: interpeduncular nucleus	Weak
TS24: lateral habenular nucleus	Weak
TS24: medial habenular nucleus	Weak

Specimen Table S5 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Weak
TS28: corpus striatum	Absent
TS28: hypothalamus lateral zone	Absent
TS28: interpeduncular nucleus	Absent
TS28: lateral habenular nucleus	Absent
TS28: medial habenular nucleus	Absent
TS28: paraventricular thalamic nucleus	Absent
TS28: substantia nigra	Absent
TS28: thalamus	Absent
TS28: ventral tegmental area	Absent

Specimen Table S5 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Weak
TS28: medial habenular nucleus	Weak
TS28: telencephalon septum	Weak
TS28: thalamus	Weak
TS28: corpus striatum	Absent
TS28: hypothalamus lateral zone	Absent
TS28: interpeduncular nucleus	Absent
TS28: substantia nigra	Absent
TS28: ventral tegmental area	Absent
TS28: paraventricular thalamic nucleus	Moderate

13B ©

Specimen 13B: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Present		13B

13F ©

Specimen 13F: postnatal day 5 (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Present	13F
TS28: cerebellum external granule cell layer	Present	13F
TS28: cerebellum internal granule cell layer	Present	13F

Specimen Table S6 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Strong
TS24: cerebellum Purkinje cell cluster	Moderate
TS24: deep cerebellar nucleus	Moderate
TS24: cerebellum ventricular layer	Weak

Specimen Table S6 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum external granule cell layer	Moderate	Regionally restricted	Expression was detected in inner EGL layers.
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum internal granule cell layer	Moderate

Specimen Table S6 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum granule cell layer	Moderate
TS28: cerebellum molecular layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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