Rgma RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199400 van den Heuvel DM, et al., Spatiotemporal expression of repulsive guidance molecules (RGMs) and their receptor neogenin in the mouse brain. PLoS One. 2013;8(2):e55828
Assay type:	RNA in situ
MGI Accession ID:	MGI:5693170
Gene symbol:	Rgma
Gene name:	repulsive guidance molecule family member A

Probe:	Rgma cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A ©

1A' ©

Specimen 1A: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	1A , 1A'	Strongest expression was detected in the apical part of the olfactory epithelium.
TS24: main olfactory bulb mitral cell layer	Present	Regionally restricted	1A	Expression was detected in a dorsomedial subset of mitral cells.
TS24: accessory olfactory bulb	Present		1A
TS24: main olfactory bulb ventricular layer	Present		1A

1L ©

1L' ©

Specimen 1L: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Present		1L	Expression was as observed at E16.5.
TS28: main olfactory bulb glomerular layer	Strong	Regionally restricted	1L' , 1L	Strong expression was detected in periglomerular cells.
TS28: main olfactory bulb mitral cell layer	Strong		1L' , 1L

Specimen Table S2 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: olfactory epithelium	Strong	Regionally restricted	Apical expression.
TS24: ethmoid bone cribriform plate	Absent
TS24: accessory olfactory bulb	Strong
TS24: main olfactory bulb ventricular layer	Strong
TS24: main olfactory bulb internal plexiform layer	Moderate
TS24: main olfactory bulb prospective glomerular layer	Moderate
TS24: main olfactory bulb prospective granule cell layer	Moderate
TS24: main olfactory bulb external plexiform layer	Weak
TS24: main olfactory bulb mitral cell layer	Very strong

Specimen Table S2 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Note
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate
TS28: main olfactory bulb external plexiform layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: olfactory epithelium	Moderate	Regionally restricted	Apical expression.
TS28: anterior olfactory nucleus	Strong
TS28: ethmoid bone cribriform plate	Absent

Specimen Table S2 adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb external plexiform layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: anterior olfactory nucleus	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate

3A ©

3A' ©

Specimen 3A: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebral cortex	Strong	3A , 3A'
TS24: cerebral cortex ventricular layer	Present	3A' , 3A
TS24: cortical plate	Present	3A' , 3A

4A ©

4A' ©

Specimen 4A: postnatal day 5 (more

)

Structure	Level	Image
TS28: cortical layer I	Strong	4A' , 4A
TS28: cortical layer II	Strong	4A' , 4A
TS28: cortical layer III	Strong	4A' , 4A
TS28: cortical layer V	Strong	4A' , 4A
TS28: cortical layer VI	Strong	4A' , 4A

Specimen Table S3 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Absent
TS24: cerebral cortex marginal layer	Absent
TS24: cerebral cortex subventricular zone	Absent
TS24: cortical subplate	Absent
TS24: telencephalon pia mater	Absent
TS24: cortical plate	Very strong
TS24: cerebral cortex ventricular layer	Strong

Specimen Table S3 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cortical layer VI	Strong
TS28: cortical layer IV	Absent
TS28: cortical layer V	Very strong
TS28: cerebral cortex ventricular zone	Moderate

Specimen Table S3 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Absent
TS28: cortical layer II	Absent
TS28: cortical layer III	Absent
TS28: cortical layer IV	Absent
TS28: cortical layer V	Strong
TS28: cortical layer VI	Weak
TS28: cerebral cortex ventricular zone	Moderate

6A ©

Specimen 6A: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong	Regionally restricted	6A	Strong expression was detected in neurons in the CA layers.
TS24: dentate gyrus primordium	Strong		6A	Expression was in neurons.
TS24: hippocampus ventricular layer	Strong		6A

6J ©

Specimen 6J: postnatal day 5 (more

)

Structure	Level	Image
TS28: hippocampus CA1	Strong	6J
TS28: dentate gyrus granule cell layer	Weak	6J
TS28: dentate gyrus polymorphic layer	Present	6J

Specimen Table S4 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: hippocampus	Strong	Regionally restricted	Strong expression was detected in the CA region. No expression was in the subventricular zone.
TS24: dentate gyrus primordium	Strong
TS24: entorhinal cortex	Strong
TS24: hippocampus ventricular layer	Strong

Specimen Table S4 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Moderate
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus	Moderate
TS28: dentate gyrus granule cell layer	Moderate
TS28: dentate gyrus molecular layer	Moderate
TS28: dentate gyrus polymorphic layer	Moderate
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum radiatum	Weak

Specimen Table S4 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus	Strong
TS28: dentate gyrus granule cell layer	Strong
TS28: dentate gyrus molecular layer	Absent
TS28: dentate gyrus polymorphic layer	Weak
TS28: entorhinal cortex	Weak
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Absent
TS28: hippocampus stratum radiatum	Absent

8A ©

Specimen 8A: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: medial habenular nucleus	Strong		8A
TS24: lateral habenular nucleus	Present		8A

8D ©

Specimen 8D: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: medial habenular nucleus	Strong	8D
TS24: thalamus	Strong	8D
TS24: lateral habenular nucleus	Present	8D

8N ©

Specimen 8N: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted	8N	Expression was detected in the ventral tegmental area and substantia nigra.

9A ©

Specimen 9A: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		9A
TS28: paraventricular thalamic nucleus	Present		9A

9G ©

Specimen 9G: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		9G
TS28: paraventricular thalamic nucleus	Present		9G

Specimen Table S5 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: midbrain	Strong	Regionally restricted	Expression was strong in the substantia nigra and the ventral tegmental area.
TS24: corpus striatum	Moderate
TS24: lateral habenular nucleus	Moderate
TS24: telencephalon septum	Moderate
TS24: hypothalamus	Moderate	Regionally restricted	Expression was detected in the lateral hypothalamus.
TS24: medial habenular nucleus	Very strong
TS24: thalamus	Very strong
TS24: interpeduncular nucleus	Strong

Specimen Table S5 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Strong
TS28: medial habenular nucleus	Very strong
TS28: thalamus	Very strong
TS28: hypothalamus lateral zone	Moderate
TS28: interpeduncular nucleus	Moderate
TS28: paraventricular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: telencephalon septum	Moderate
TS28: ventral tegmental area	Moderate
TS28: corpus striatum	Weak

Specimen Table S5 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Moderate
TS28: paraventricular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: telencephalon septum	Moderate
TS28: thalamus	Moderate
TS28: ventral tegmental area	Moderate
TS28: interpeduncular nucleus	Strong
TS28: medial habenular nucleus	Strong
TS28: hypothalamus lateral zone	Weak
TS28: corpus striatum	Absent

11A ©

Specimen 11A: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebellum Purkinje cell cluster	Present	11A
TS24: cerebellum ventricular layer	Present	11A
TS24: deep cerebellar nucleus	Present	11A
TS24: cerebellum external granule cell layer	Absent	11A

11G ©

Specimen 11G: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		11G
TS28: cerebellum Purkinje cell layer	Absent		11G

11M ©

Specimen 11M: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11M
TS28: cerebellum granule cell layer	Present		11M

Specimen Table S6 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum ventricular layer	Strong
TS24: deep cerebellar nucleus	Strong
TS24: cerebellum Purkinje cell cluster	Moderate
TS24: cerebellum external granule cell layer	Absent

Specimen Table S6 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Very strong
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum external granule cell layer	Absent

Specimen Table S6 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum granule cell layer	Moderate
TS28: cerebellum molecular layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/30/2024 MGI 6.23