Rgma RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199400 van den Heuvel DM, et al., Spatiotemporal expression of repulsive guidance molecules (RGMs) and their receptor neogenin in the mouse brain. PLoS One. 2013;8(2):e55828
Assay type:	RNA in situ
MGI Accession ID:	MGI:5693170
Gene symbol:	Rgma
Gene name:	repulsive guidance molecule family member A

Probe:	Rgma cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A ©

1A' ©

Specimen 1A: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	1A , 1A'	Strongest expression was detected in the apical part of the olfactory epithelium.
TS24: main olfactory bulb mitral cell layer	Present	Regionally restricted	1A	Expression was detected in a dorsomedial subset of mitral cells.
TS24: accessory olfactory bulb	Present		1A
TS24: main olfactory bulb ventricular layer	Present		1A

1L ©

1L' ©

Specimen 1L: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Present		1L	Expression was as observed at E16.5.
TS28: main olfactory bulb glomerular layer	Strong	Regionally restricted	1L' , 1L	Strong expression was detected in periglomerular cells.
TS28: main olfactory bulb mitral cell layer	Strong		1L' , 1L

Specimen Table S2 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: olfactory epithelium	Strong	Regionally restricted	Apical expression.
TS24: ethmoid bone cribriform plate	Absent
TS24: accessory olfactory bulb	Strong
TS24: main olfactory bulb ventricular layer	Strong
TS24: main olfactory bulb internal plexiform layer	Moderate
TS24: main olfactory bulb prospective glomerular layer	Moderate
TS24: main olfactory bulb prospective granule cell layer	Moderate
TS24: main olfactory bulb external plexiform layer	Weak
TS24: main olfactory bulb mitral cell layer	Very strong

Specimen Table S2 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Note
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate
TS28: main olfactory bulb external plexiform layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: olfactory epithelium	Moderate	Regionally restricted	Apical expression.
TS28: anterior olfactory nucleus	Strong
TS28: ethmoid bone cribriform plate	Absent

Specimen Table S2 adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb external plexiform layer	Weak
TS28: main olfactory bulb internal plexiform layer	Weak
TS28: anterior olfactory nucleus	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: main olfactory bulb ventricular layer	Moderate

3A ©

3A' ©

Specimen 3A: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebral cortex	Strong	3A , 3A'
TS24: cerebral cortex ventricular layer	Present	3A' , 3A
TS24: cortical plate	Present	3A' , 3A

4A ©

4A' ©

Specimen 4A: postnatal day 5 (more

)

Structure	Level	Image
TS28: cortical layer I	Strong	4A' , 4A
TS28: cortical layer II	Strong	4A' , 4A
TS28: cortical layer III	Strong	4A' , 4A
TS28: cortical layer V	Strong	4A' , 4A
TS28: cortical layer VI	Strong	4A' , 4A

Specimen Table S3 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Absent
TS24: cerebral cortex marginal layer	Absent
TS24: cerebral cortex subventricular zone	Absent
TS24: cortical subplate	Absent
TS24: telencephalon pia mater	Absent
TS24: cortical plate	Very strong
TS24: cerebral cortex ventricular layer	Strong

Specimen Table S3 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cortical layer VI	Strong
TS28: cortical layer IV	Absent
TS28: cortical layer V	Very strong
TS28: cerebral cortex ventricular zone	Moderate

Specimen Table S3 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Absent
TS28: cortical layer II	Absent
TS28: cortical layer III	Absent
TS28: cortical layer IV	Absent
TS28: cortical layer V	Strong
TS28: cortical layer VI	Weak
TS28: cerebral cortex ventricular zone	Moderate

6A ©

Specimen 6A: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong	Regionally restricted	6A	Strong expression was detected in neurons in the CA layers.
TS24: dentate gyrus primordium	Strong		6A	Expression was in neurons.
TS24: hippocampus ventricular layer	Strong		6A

6J ©

Specimen 6J: postnatal day 5 (more

)

Structure	Level	Image
TS28: hippocampus CA1	Strong	6J
TS28: dentate gyrus granule cell layer	Weak	6J
TS28: dentate gyrus polymorphic layer	Present	6J

Specimen Table S4 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: hippocampus	Strong	Regionally restricted	Strong expression was detected in the CA region. No expression was in the subventricular zone.
TS24: dentate gyrus primordium	Strong
TS24: entorhinal cortex	Strong
TS24: hippocampus ventricular layer	Strong

Specimen Table S4 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Moderate
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus	Moderate
TS28: dentate gyrus granule cell layer	Moderate
TS28: dentate gyrus molecular layer	Moderate
TS28: dentate gyrus polymorphic layer	Moderate
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Weak
TS28: hippocampus stratum radiatum	Weak

Specimen Table S4 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus	Strong
TS28: dentate gyrus granule cell layer	Strong
TS28: dentate gyrus molecular layer	Absent
TS28: dentate gyrus polymorphic layer	Weak
TS28: entorhinal cortex	Weak
TS28: hippocampus stratum lacunosum	Weak
TS28: hippocampus stratum oriens	Absent
TS28: hippocampus stratum radiatum	Absent

8A ©

Specimen 8A: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: medial habenular nucleus	Strong		8A
TS24: lateral habenular nucleus	Present		8A

8D ©

Specimen 8D: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: medial habenular nucleus	Strong	8D
TS24: thalamus	Strong	8D
TS24: lateral habenular nucleus	Present	8D

8N ©

Specimen 8N: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted	8N	Expression was detected in the ventral tegmental area and substantia nigra.

9A ©

Specimen 9A: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		9A
TS28: paraventricular thalamic nucleus	Present		9A

9G ©

Specimen 9G: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		9G
TS28: paraventricular thalamic nucleus	Present		9G

Specimen Table S5 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: midbrain	Strong	Regionally restricted	Expression was strong in the substantia nigra and the ventral tegmental area.
TS24: corpus striatum	Moderate
TS24: lateral habenular nucleus	Moderate
TS24: telencephalon septum	Moderate
TS24: hypothalamus	Moderate	Regionally restricted	Expression was detected in the lateral hypothalamus.
TS24: medial habenular nucleus	Very strong
TS24: thalamus	Very strong
TS24: interpeduncular nucleus	Strong

Specimen Table S5 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Strong
TS28: medial habenular nucleus	Very strong
TS28: thalamus	Very strong
TS28: hypothalamus lateral zone	Moderate
TS28: interpeduncular nucleus	Moderate
TS28: paraventricular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: telencephalon septum	Moderate
TS28: ventral tegmental area	Moderate
TS28: corpus striatum	Weak

Specimen Table S5 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Moderate
TS28: paraventricular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: telencephalon septum	Moderate
TS28: thalamus	Moderate
TS28: ventral tegmental area	Moderate
TS28: interpeduncular nucleus	Strong
TS28: medial habenular nucleus	Strong
TS28: hypothalamus lateral zone	Weak
TS28: corpus striatum	Absent

11A ©

Specimen 11A: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: cerebellum Purkinje cell cluster	Present	11A
TS24: cerebellum ventricular layer	Present	11A
TS24: deep cerebellar nucleus	Present	11A
TS24: cerebellum external granule cell layer	Absent	11A

11G ©

Specimen 11G: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		11G
TS28: cerebellum Purkinje cell layer	Absent		11G

11M ©

Specimen 11M: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11M
TS28: cerebellum granule cell layer	Present		11M

Specimen Table S6 E16.5: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum ventricular layer	Strong
TS24: deep cerebellar nucleus	Strong
TS24: cerebellum Purkinje cell cluster	Moderate
TS24: cerebellum external granule cell layer	Absent

Specimen Table S6 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Very strong
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum external granule cell layer	Absent

Specimen Table S6 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong
TS28: cerebellum granule cell layer	Moderate
TS28: cerebellum molecular layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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