Probe:	Gas1 probe2
Probe preparation:	labelled with digoxigenin

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Present		1A

Structure	Level	Pattern	Image	Note
TS12: somite	Present		1B
TS12: future prosencephalon	Present	Regionally restricted	1B	Expression was primarily localized to the alar region and devoid from the ventral midline.

Structure	Level	Pattern	Image	Note
TS12: somite	Present		1C
TS12: trunk unsegmented mesenchyme	Absent		1C
TS12: future spinal cord	Present		1C
TS12: future prosencephalon	Present	Regionally restricted	1C	Expression was primarily localized to the alar region and devoid from the ventral midline.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		1D
TS15: mesenchyme derived from lateral plate	Present		1D
TS15: optic vesicle	Present		1D
TS15: otocyst	Present		1D
TS15: somite	Present		1D
TS15: tail	Absent		1D
TS15: tail unsegmented mesenchyme	Absent		1D
TS15: future spinal cord	Present	Regionally restricted	1D	Expression was dorsally restricted.
TS15: diencephalon	Weak	Regionally restricted	1D	Weak expression was detected in dorsal diencephalon.
TS15: future hindbrain	Weak		1D
TS15: future midbrain	Weak		1D
TS15: telencephalon	Present	Regionally restricted	1D	Expression was detected in the dorsomedial region.

Structure	Level	Pattern	Image	Note
TS16: branchial arch	Present		1E
TS16: mesenchyme derived from lateral plate	Present		1E
TS16: somite	Present		1E
TS16: tail	Absent		1E
TS16: tail unsegmented mesenchyme	Absent		1E
TS16: future spinal cord	Present	Regionally restricted	1E	Expression was dorsally restricted.
TS16: diencephalon	Weak	Regionally restricted	1E	Weak expression was detected in dorsal diencephalon.
TS16: future hindbrain	Weak		1E
TS16: future midbrain	Weak		1E
TS16: telencephalon	Present	Regionally restricted	1E	Expression was detected in the dorsomedial region.

Structure	Level	Pattern	Image	Note
TS19: somite	Present		1F
TS19: dermatome	Present	Regionally restricted	1F	Expression was detected in trunk dermatome cells as they disperse to populate the dermal layer of the skin.
TS19: future spinal cord	Present	Regionally restricted	1F	Expression was dorsally restricted.
TS19: tail	Absent		1F
TS19: diencephalon	Weak	Regionally restricted	1F	Weak expression was detected in dorsal diencephalon.
TS19: hindbrain	Weak		1F
TS19: midbrain	Weak		1F
TS19: telencephalon	Present	Regionally restricted	1F	Expression was detected in the dorsomedial region.

Structure	Level	Pattern	Image	Note
TS20: somite	Present		1G
TS20: spinal cord	Absent		1G
TS20: vibrissa follicle	Absent		1G
TS20: neocortex	Present		1G
TS20: telencephalon	Present	Regionally restricted	1G	Expression was detected in the dorsomedial region.
TS20: dermatome	Present	Regionally restricted	1G	Expression was detected in trunk dermatome cells as they disperse to populate the dermal layer of the skin.

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Strong		1H
TS15: myotome	Weak		1H
TS15: sclerotome	Weak		1H
TS15: embryo endoderm	Absent		1H
TS15: mesenchyme derived from splanchnopleure	Absent		1H
TS15: future spinal cord	Present	Regionally restricted	1H	Expression was dorsally restricted.
TS15: mesenchyme derived from lateral plate	Present		1H

Structure	Level	Pattern	Image	Note
TS14: forelimb bud	Present	Regionally restricted	1J	Anterior-specific expression was detected.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	1K	Anterior-specific expression was detected.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	1L	Anterior-specific expression was detected.

Structure	Level	Pattern	Image	Note
TS20: limb	Present		1M

Structure	Level	Pattern	Image	Note
TS20: limb	Present		1N

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	1O	Expression was detected in cells next to the condensing cartilage.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	1P	Expression was detected in cells next to the condensing cartilage.

Structure	Level	Pattern	Image	Note
TS22: hand mesenchyme	Present	Regionally restricted	1Q	Expression was detected in the mesenchyme surrounding and between digits and metacarpals.
TS22: forelimb interdigital region mesenchyme	Present		1Q