Probe:	pSPmCHL2-COOH
Probe preparation:	Antisense RNA
Assay notes:	Two probes were described MGI:5691692 and MGI:5691693; the probe used in this assay was not detailed. The fixative was zinc-formalin.

Structure	Level	Pattern	Image	Note
TS25: costo-chondral joint	Present	Regionally restricted	4A	Expression was detected in the surface chondrocytes of developing joint cartilage. Expression was in chondrocytes on both sides of the costochondral junction.

Structure	Level	Pattern	Image	Note
TS26: sternum	Present	Regionally restricted	4B	Expression was detected in areas where ribs converge.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Weak	Regionally restricted	4D , 4C	Weak expression was detected over the articular cartilage surface of the femoral head and patella. Expression was seen in chondrocytes on both sides of the joint.
TS28: long bone epiphyseal plate	Absent		4C , 4D

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	4E , 4F	Expression was detected in the superficial articular chondrocytes on both sides of the zygapophyseal joint.

Structure	Level	Pattern	Image	Note
TS28: myometrium	Strong		5Ba

Structure	Level	Pattern	Image	Note
TS28: colon	Trace	Regionally restricted	5Bb	Trace signal was found on colonic serosa.

Structure	Level	Pattern	Image	Note
TS28: annulus fibrosus	Weak

Structure	Level	Pattern	Image	Note
TS28: placenta	Present	Regionally restricted		Expression was detected in maternally-derived placental tissue.