Probe:	Sall3 cDNA2
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography
Assay notes:	Probe was hydrolyzed to a final length of approximately 200 bases.

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong		5A
TS11: neural ectoderm	Present	Graded	5A	High expression from the allantois to the node and weaker from the node to the headfold.
TS11: primitive streak	Present	Regionally restricted	5A	Expression is in mesoderm posterior to the primitive node.
TS11: embryo endoderm	Absent		5A
TS11: notochordal plate	Absent		5A

Structure	Level	Pattern	Image	Note
TS12: future brain	Strong		5B
TS12: future spinal cord	Present		5B
TS12: 1st branchial arch mesenchyme	Weak		5B

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	5C	Expression is in the mesenchymal progress zone.
TS16: forelimb bud apical ectodermal ridge	Absent		5C

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present	Regionally restricted	5D	Expression retracts from the dorsal epithelium.
TS16: future forebrain	Strong	Regionally restricted	5D	Expression is ventral extending from the optic recess to the spinal cord.
TS16: future hindbrain	Strong	Regionally restricted	5D	Expression is ventral extending from the optic recess to the spinal cord.
TS16: future midbrain	Strong	Regionally restricted	5D	Expression is ventral extending from the optic recess to the spinal cord.
TS16: 1st branchial arch mesenchyme	Weak		5D
TS16: 2nd branchial arch mesenchyme	Weak		5D

Structure	Level	Pattern	Image	Note
TS14: neural tube lateral wall	Present	Regionally restricted	6A	Expression is throughout the lateral wall except in cells of the intermediate zone.
TS14: notochord	Weak		6A
TS14: neural tube floor plate	Absent		6A

Structure	Level	Pattern	Image	Note
TS16: neural tube lateral wall	Present	Regionally restricted	6B	Expression is in the ventricular zone.
TS16: notochord	Weak		6B
TS16: neural tube floor plate	Absent		6B

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Strong	Regionally restricted	6C	Expression is mostly dorsal.
TS20: spinal cord roof plate	Present		6C
TS20: spinal cord lateral wall	Present	Regionally restricted	6C	Expression is in cell clusters in the basal and intermediate parts.
TS20: spinal cord floor plate	Absent		6C

Structure	Level	Pattern	Image	Note
TS18: otocyst	Strong	Regionally restricted	7A	Expression is in the ventro-medial part.
TS18: acoustic ganglion	Strong		7A
TS18: facial ganglion	Strong		7A

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	7B	Expression is in the ventro-medial part of the otic vesicle.
TS20: inner ear	Present	Regionally restricted	7B	Expression is in the ventro-medial part of the otic vesicle.
TS20: facial ganglion	Strong		7B
TS20: vestibulo-cochlear ganglion	Strong		7B

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present		7C
TS22: vestibulo-cochlear ganglion	Absent		7C

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	7D	Expression is in the epithelial ridge that form the sensory hair cells.
TS28: vestibular epithelium	Present		7D
TS28: cochlear ganglion	Absent		7D	Absent in the branches of the ganglia.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong	Regionally restricted	8A	Strong in the hypothalamus, the ventral and dorsal thalamus with a sharp boundary at the posterior commissure, absent in the epithalamus.
TS19: midbrain	Strong	Regionally restricted	8A
TS19: telencephalon	Weak	Regionally restricted	8A	Expression is in the ventral-medial portion.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	8B	Restricted expression including the ganglionic eminences.
TS19: diencephalon	Present		8B
TS19: myelencephalon	Present	Regionally restricted	8B	At the level of the optic stalk expression is in a superficial cell layer and in two domains symmetrically along the ventral midline.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	8C	Expression is in the hypothalamus and in the posterior preoptic area.
TS19: eye	Absent		8C
TS19: optic stalk	Absent		8C

Structure	Level	Pattern	Image	Note
TS28: thalamic nucleus	Present		9A
TS28: brain ventricular layer	Present	Regionally restricted	9A	Expression is adjacent to the lateral and third ventricle.

Structure	Level	Pattern	Image	Note
TS28: mammillary body	Strong		9B
TS28: trigeminal nerve	Weak		9B

Structure	Level	Pattern	Image	Note
TS28: brainstem	Strong	Regionally restricted	9C	Expression is in the dorsal portion.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted	9D	Expression is in the periglomerular layer.

Structure	Level	Pattern	Image	Note
TS10: neural ectoderm	Present
TS10: primitive streak	Present

Structure	Level	Pattern	Image	Note
TS12: notochord	Weak

Structure	Level	Pattern	Image	Note
TS14: heart	Weak	Regionally restricted		Expression is in the truncus arteriosus.

Structure	Level	Pattern	Image	Note
TS16: mesonephric tubule	Present

Structure	Level	Pattern	Image	Note
TS18: metanephros	Present	Regionally restricted		Expression is in the metanephric duct.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted		Expression is in the dorsal thalamus, in the pretectum, the boundary between the dorsal thalamus and epithalamus, and is absent in the epithalamus and the dorsal mesencephalon.

Structure	Level	Pattern	Image	Note
TS20: heart	Weak	Regionally restricted		Expression is in the truncus arteriosus.
TS20: limb	Absent

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present