Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tfcp2l1tm1.1(cre/ERT2)Ovi
Name:	transcription factor CP2-like 1; targeted mutation 1.1, Catherine E Ovitt
MGI ID:	MGI:5662395
Synonyms:	Tcfcp2l1GCE, Tcf-GCE, Tcftm1(GCE)Ovi, Tfcp2l1tm1(cre/ERT2)Ovi
Gene:	Tfcp2l1  Location: Chr1:118555675-118612898 bp, + strand  Genetic Position: Chr1, 52.14 cM, cytoband E2
Alliance:	Tfcp2l1tm1.1(cre/ERT2)Ovi page

Germline Transmission:	Earliest citation of germline transmission: J:229520
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Inducible, Recombinase, Reporter) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Tfcp2l1tm1.1(cre/ERT2)Ovi expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Tfcp2l1 (MGI:2444691)   Mutation details: The targeting construct was generated by inserting the diphtheria toxin A gene (DTA, for positive selection), 3.2 kb 5 homologous arm containing the 5' UTR of exon 1, and 5.5 kb 3 homologous arm containing exon 2 into pBluescript SKII(+). Then the eGFP-CreERT2 (GCE) fragment (McMahon et al. 2008) with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. (J:229520)

Activity:	Tissue activity of this recombinase allele
Driver:	Tfcp2l1 (mouse) Summary of all recombinase alleles driven by Tfcp2l1. Comparative matrix view of recombinase activities Alliance mouse Tfcp2l1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tfcp2l1 Mutation:	99 strains or lines available

Original:	J:229520 Maruyama EO, et al., Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands. PLoS One. 2016;11(1):e0146711
All:	2 reference(s)