Probe:	Msx-3/SX
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present			Expression is in a segmented pattern.
TS12: neural tube	Present	Regionally restricted		Expression is in the dorsal third of the neural tube (anterior to posterior).
TS12: future spinal cord neural plate	Present	Regionally restricted		Expression is in the lateral neural plate.
TS12: future midbrain	Absent
TS12: future prosencephalon	Absent

Structure	Level	Pattern	Image	Note
TS12: rhombomere	Present	Regionally restricted	2A	Expression is in rhombomeres 1, 2, 4, and caudally from 6. Absent in rhombomere 3 and 5.
TS12: neural tube	Present	Regionally restricted	2A	Expression is in the dorsal third of the neural tube.
TS12: future midbrain	Absent		2A
TS12: future prosencephalon	Absent		2A

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Present		2B
TS13: rhombomere 2	Present		2B
TS13: rhombomere 4	Present		2B
TS13: rhombomere 3	Weak		2B
TS13: future midbrain	Absent		2B
TS13: future prosencephalon	Absent		2B
TS13: rhombomere 5	Absent		2B

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Uniform	2C
TS14: future spinal cord	Present	Regionally restricted	2C	Uniform expression in the anterior spinal cord.
TS14: future midbrain	Absent		2C
TS14: prosencephalon	Absent		2C

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	3A	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3C , 3B	Expression is dorsal.
TS13: future hindbrain neural crest	Absent		3C , 3B

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong	Regionally restricted	4A	Expression is dorsal.
TS15: future spinal cord	Strong	Regionally restricted	4A	Expression is dorsal.
TS15: future midbrain	Absent		4A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4B	Expression is dorsal.
TS17: rhombomere 2	Present	Regionally restricted	4B	Expression is dorsal.
TS17: rhombomere 5	Present	Regionally restricted	4B	Expression is dorsal.
TS17: rhombomere 3	Weak		4B	Expression is dorsal.
TS17: rhombomere 4	Weak		4B	Expression is dorsal.
TS17: midbrain	Absent		4B

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4C	Expression is dorsal and is rhombomeres 1, and 2, and caudally in 6, and is absent in rhombomeres 3, 4, and 5.
TS19: future spinal cord	Present	Regionally restricted	4C	Expression is in the anterior spinal cord.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present		4D	Expression is dorsal.
TS15: trunk mesenchyme	Present	Single cells	4D	Expression was in a few cells adjacent to the expression domain in the spinal cord.
TS15: neural tube roof plate	Absent		4D

Structure	Level	Pattern	Image	Note
TS15: future hindbrain roof plate	Present	Regionally restricted		Expression is in the anterior hindbrain.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	5A	Expression is dorsal.
TS17: neural tube roof plate	Absent		5A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	5B	Expression is dorsal.
TS17: neural tube roof plate	Absent		5B

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Very strong	Regionally restricted	5C	Section through anterior spinal cord. Expression is very strong dorsallay and a weaker domain is seen ventral to this area.
TS17: neural tube roof plate	Absent		5C

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	5D	Section through posterior hindbrain. Expression is very strong dorsallay and a weaker domain is seen ventral to this area.
TS17: hindbrain roof plate	Absent		5D

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4 ventricular layer	Present	Regionally restricted	5E	Segmented dorsal expression.
TS17: rhombomere 4 roof plate	Absent		5E

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Present	Regionally restricted	5F	Expression is dorsal.
TS17: rhombomere 2 roof plate	Absent		5F

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent

Structure	Level	Pattern	Image	Note
TS21: embryo	Absent