Gene Expression Data
Assay Details
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Assay
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Reference:
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J:182578
Kay JN, et al., MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons. Nature. 2012 Mar 22;483(7390):465-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5660813
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Gene symbol:
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Megf10
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Gene name:
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multiple EGF-like-domains 10
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Probe:
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MGC clone 100091
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1c:
postnatal day 5
(more  )
Note: Right image is double labeled: red - Megf10; green - Calb1 (assay MGI:5660812).
Specimen
1c:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Right image is double labeled: red - Megf10; green - Calb1 (assay MGI:5660812).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
1c right
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1c left
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Expression was detected in starburst amacrine cells and horizontal cells.
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| TS28: retina ganglion cell layer |
Present |
Regionally restricted |
1c right
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1c left
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Expression was detected in starburst amacrine cells.
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Specimen
1d:
embryonic day 16.0
(more )
Note: Double labeled: red - Megf10; green - Isl1 (assay MGI:5660814).
Specimen
1d:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: red - Megf10; green - Isl1 (assay MGI:5660814).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina nuclear layer |
Present |
Regionally restricted |
1d left
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1d right
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Expression was detected in starburst amacrine cells (SAC) in the inner nuclear layer. Migrating SACs in the outer neuroblast layer, which are positive for Isl1, were not labeled.
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Specimen
1e:
postnatal day 14
(more )
Specimen
1e:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
1e
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Expression in the second postnatal week was downregulated in neurons but appeared in Muller glia.
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Specimen
S1b:
postnatal day 7
(more )
Note: Right image is double labeled: red - Megf10; green - Calb1 (assay MGI:5660812).
Specimen
S1b:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Note:
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Right image is double labeled: red - Megf10; green - Calb1 (assay MGI:5660812).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
S1b left
,
S1b right
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Expression was detected in starburst amacrine cells, horizontal cells, and Muller glia.
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| TS28: retina ganglion cell layer |
Present |
Regionally restricted |
S1b right
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S1b left
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Expression was detected in starburst amacrine cells.
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