Gene Expression Data
Assay Details
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Assay
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Reference:
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J:20160
Kurtz A, et al., The expression pattern of a novel gene encoding brain-fatty acid binding protein correlates with neuronal and glial cell development. Development. 1994 Sep;120(9):2637-49
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5649420
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Gene symbol:
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Map2
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Gene name:
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microtubule-associated protein 2
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Antibody:
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anti-MAP2
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
10B:
embryonic day 11.5
(more  )
Note: Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
Specimen
10B:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain lateral wall |
Present |
Regionally restricted |
10B
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Expressed in a thin superficial layer of neuronal cells, which is only above the Fabp7-positive region of the ventricular zone.
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Specimen
10D:
embryonic day 12.5
(more )
Note: Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
Specimen
10D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain lateral wall |
Present |
Regionally restricted |
10D
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Expressed in a broad layer of neuronal cells in the corresponding intermediate zone.
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Specimen
10F:
embryonic day 14.5
(more )
Note: Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
Specimen
10F:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain lateral wall |
Present |
Regionally restricted |
10F
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Expressed in a broad layer of neuronal cells in the corresponding intermediate zone.
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Specimen
11B:
embryonic day 11.5
(more )
Note: Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
Specimen
11B:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
11B
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Expresse in the striatum and ventrolateral domain of the lateral ventricle.
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Specimen
11C:
embryonic day 12.5
(more )
Note: Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
Specimen
11C:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
11C
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Expressed in the intermediate zones.
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| TS20: hypothalamus ventricular layer |
Present |
Regionally restricted |
11C
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Expressed in neuronal cells, which appears first in areas where the ventricular zone cells contain high levels of Fabp7.
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Specimen
11D:
embryonic day 12.5
(more )
Note: Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
Specimen
11D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Map2; red - Fabp7 (assay MGI:5649405).
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Analysis Tools