Igdcc3 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70796 Yang W, et al., Impaired motor coordination in mice that lack punc. Mol Cell Biol. 2001 Sep;21(17):6031-43
Assay type:	Northern blot
MGI Accession ID:	MGI:5643743
Gene symbol:	Igdcc3
Gene name:	immunoglobulin superfamily, DCC subclass, member 3

Probe:	3' punc probe
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	These data were discussed but not shown.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	5 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E11.5	TS19: embryo	Present	5 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Not Specified
+/-	E11.5	TS19: embryo	Present (a)	5 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Igdcc3^tm1.1Sms/Igdcc3⁺	Not Specified
-/-	E11.5	TS19: embryo	Absent (b)	5 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Igdcc3^tm1.1Sms/Igdcc3^tm1.1Sms	Not Specified

Notes:
(a) Expression levels in the het are the same as those seen in wild type.
(b) There are low levels of a small transcript in this lane. Other experiments indicate this initiates from within the lacZ sequence but as there is no in-frame consensus translation initiation codon downstream, the allele is likely to be functionally null.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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