Reporter:	GFP
Antibody:	anti-GFP (ab6556)
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	2B	High levels of expression throughout entire dorsal pallium (isocortex) and paraseptal subpallium.
TS21: neocortex	Present		2B
TS21: thalamus	Present	Regionally restricted	2B	Expression in the alar plate of thalamus.
TS21: hypothalamus	Strong	Regionally restricted	2B	Expression in the basal part of terminal hypothalamus.
TS21: forebrain-midbrain boundary region	Present		2B	Expression in the commissural pretectal region.
TS21: hindbrain	Absent		2B	No expression in structures extending from the prospective prepontine hindbrain to the medullary hindbrain.
TS21: spinal cord	Present		2B	Expression in ventral and dorsal layers of spinal cord.
TS21: dorsal aorta	Present		2B
TS21: heart atrium	Present		2B
TS21: heart ventricle	Present		2B
TS21: liver	Present		2B
TS21: lung	Present		2B
TS21: tongue	Present		2B

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Present		2C	Expression mostly restricted to ventral pallium (olfactory bulb), dorsal pallium (gives rise to frontal, occipital, insular and entorhinal cortices) and medial pallium (gives rise to hippocampus and subicullum).
TS23: main olfactory bulb	Present		2C	Expression mostly restricted to ventral pallium (olfactory bulb), dorsal pallium (gives rise to frontal, occipital, insular and entorhinal cortices) and medial pallium (gives rise to hippocampus and subicullum).
TS23: cerebral cortex ventricular layer	Present	Regionally restricted	2C	In dorsal pallium, expression in ventricular zone and superficial strata, but not in the intermediate stratum.
TS23: neocortex	Present	Regionally restricted	2C	In dorsal pallium, expression in ventricular zone and superficial strata, but not in the intermediate stratum.
TS23: diencephalon	Absent		2C
TS23: hindbrain	Absent		2C
TS23: midbrain	Absent		2C
TS23: bladder	Present		2C
TS23: genital tubercle	Present		2C
TS23: heart ventricle	Present		2C
TS23: intestine	Present		2C
TS23: liver	Present		2C
TS23: lung	Present		2C
TS23: metanephros	Present		2C
TS23: pancreas	Present		2C
TS23: salivary gland	Present		2C
TS23: thymus primordium	Present		2C

Structure	Level	Pattern	Image	Note
TS26: brain subventricular zone	Trace		2D	Very slight expression in subventricular zone.
TS26: brown fat	Present		2D
TS26: heart atrium	Present		2D
TS26: intestine epithelium	Present		2D
TS26: liver	Present		2D
TS26: lung	Present		2D
TS26: thymus	Present		2D
TS26: metanephros	Present		2D	Authors report kidney maintains similar expression from E13.5 to E18.5
TS26: salivary gland	Ambiguous		2D	Expression in salivary glands diminishes as embryo matures.

Structure	Level	Pattern	Image	Note
TS26: bladder	Weak			Authors state that expression in bladder diminishes near birth.
TS26: genital tubercle	Weak			Authors state that expression in the genital ridge diminishes at birth.

Structure	Level	Pattern	Image	Note
TS23: dermis	Present		2E	Expression in dermal cells.
TS23: epidermis	Present	Regionally restricted	2E	Expression in basal cell compartment.
TS23: hair follicle	Absent		2E	No expression in hair follicle buds at E15.5.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present		2F	Expression in dermal cells.
TS24: epidermis	Present	Regionally restricted	2F	Expression in basal cell compartment.
TS24: hair follicle	Strong		2F	Abundant expression in hair follicle buds at E16.5.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present		2G	Authors report kidney maintains similar expression from E13.5 to E18.5
TS21: cartilage	Present		2G
TS21: muscle tissue	Present		2G
TS21: spinal cord	Present		2G

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present		2H	Authors report kidney maintains similar expression from E13.5 to E18.5
TS26: skeletal muscle	Ambiguous		2H	Expression diminishes in myocytes.
TS26: spinal cord	Ambiguous		2H	Expression diminishes in spinal cord.
TS26: cartilage	Ambiguous		2H	Expression diminishes in chrondrocytes vertebrae.

Structure	Level	Pattern	Image	Note
TS26: vertebra	Present	Regionally restricted		Expression in osteoblasts and osteoclasts around foci of endochondral ossification.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Widespread	4A	Diffuse expression in most liver cell types.
TS28: liver parenchyma	Present	Widespread	4A	Expression in virtually all hepatocytes
TS28: bile duct	Present	Regionally restricted	4A	Expression in bile ductular epithelium.

Structure	Level	Pattern	Image	Note
TS28: alveolar septum	Strong	Regionally restricted	4C	Abundant expression in alveolar septal endothelial cells.
TS28: lung vascular element	Present	Regionally restricted	4C	Expression in pulmonary endothelia including those lining blood vessels.
TS28: alveolus epithelium	Absent		4C	No expression in alveolar pneumocytes.
TS28: bronchiole epithelium	Absent		4C

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Strong	Uniform	4E	Abundant and uniform expression in exocrine acinar cells.
TS28: islets of Langerhans	Present	Non-Uniform	4E	Heterogeneous expression in pancreatic islet cells.
TS28: pancreatic duct	Present	Regionally restricted	4E	Heterogeneous expression in pancreatic ductular epithelia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: testis		Present	Regionally restricted	4G	Expression in testis, higher in type B spermatogonia and spermatocytes.
TS28: spermatogonium		Present	Regionally restricted	4G	Expression in adluminal type B spermatogonia. Variable expression in type A spermatogonia at the margins of seminiferous tubules.
TS28: spermatocyte		Weak	Non-Uniform	4G	Variable and less pronounced expression in spermatocytes.
TS28: Sertoli cell	Sertoli cell	Present	Non-Uniform	4G	Heterogeneous expression in a few Sertoli cells.
TS28: interstitium of the testis		Present	Non-Uniform	4G

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Strong		5A
TS28: renal medulla	Absent		5A	No expression in medullary parenchyma.

Structure	Level	Pattern	Image	Note
TS28: renal vasculature	Present	Regionally restricted	5B	Expression in endothelial cells in arterioles.
TS28: glomerular capillary system	Present		5B	Expression in glomerular tuft endothelial cells.
TS28: visceral epithelium of mature renal corpuscle	Absent		5B	No expression in glomerular visceral podocytes.
TS28: renal proximal tubule	Strong		5B	Intense expression in proximal tubular epithelia.
TS28: distal convoluted tubule	Absent		5B	No expression in distal convoluted tubular epithelia.

Structure	Level	Pattern	Image	Note
TS28: spleen	Strong	Regionally restricted	6A	High expression in adult spleen, most abundant in macrophages in the marginal zone between red and white pulp.

Structure	Level	Pattern	Image	Note
TS28: liver sinusoid	Present			Expression in sinusoidal lining cells.
TS28: liver vascular element	Present	Regionally restricted		Expression in arteriolar smooth muscle and venules.
TS28: blood vessel smooth muscle	Present	Uniform		Authors state vascular smooth muscle myocytes were uniformly possitive in all tissues unless otherwise noted.
TS28: blood vessel endothelium	Present	Uniform		Authors state vascular endothelium was uniformly possitive in all tissues unless otherwise noted.
TS28: lung vascular element	Present	Regionally restricted		Expression in venular endothelium. No expression in bronchiolar arteriolar smooth muscle.
TS28: bronchiole smooth muscle	Absent
TS28: renal medulla	Present	Regionally restricted		Expression in medullary endothelium.
TS28: medullary collecting duct	Absent
TS28: spleen red pulp	Present
TS28: spleen white pulp	Present	Single cells		Individual cells, nonlymphoid, monocytoid, cells appear to be dendritic cells.
TS28: cardiac muscle tissue	Present	Diffuse		Diffuse expression in cardiomyocytes.
TS28: coronary artery	Present	Regionally restricted		Expression in smooth muscle.
TS28: pulmonary artery	Present	Regionally restricted		Expression in smooth muscle.
TS28: pulmonary vein	Present	Regionally restricted		Expression in smooth muscle.
TS28: thymus epithelium	Present	Regionally restricted		Expression in medullary epithelial cells.
TS28: thymus medullary core	Present	Regionally restricted		Heterogeneous expression in medullary thymocytes.
TS28: trachea epithelium	Absent			No expression in tracheal mucosa.
TS28: trachea connective tissue	Present	Regionally restricted		Heterogenous expression in perichondrium.
TS28: trachea cartilage	Absent			No expression in chondrocytes.
TS28: esophagus	Absent
TS28: skeletal muscle	Absent			No expression in myocytes.
TS28: detrusor muscle of bladder	Absent			No expression in mucosal transitional epithelium or smooth muscle.
TS28: urothelium of bladder	Absent			No expression in mucosal transitional epithelium or smooth muscle.
TS28: ureteral smooth muscle layer	Absent			No expression in mucosal transitional epithelium or smooth muscle.
TS28: urothelium of ureter	Absent			No expression in mucosal transitional epithelium or smooth muscle.
TS28: urethra epithelium	Absent			No expression in mucosal transitional epithelium.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present	Regionally restricted		Variable expression in type A spermatogonia.
TS28: ductus deferens	Absent
TS28: anterior prostate gland	Absent
TS28: dorsal prostate gland	Absent
TS28: bulbourethral gland of male	Present
TS28: lateral prostate gland	Present
TS28: ventral prostate gland	Present