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Gene Expression Data
Assay Details
Assay
Reference: J:178190 Sanematsu F, et al., DOCK180 is a Rac activator that regulates cardiovascular development by acting downstream of CXCR4. Circ Res. 2010 Oct 29;107(9):1102-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5635647
Gene symbol: Cxcr4
Gene name: C-X-C motif chemokine receptor 4
Antibody: Anti-Cxcr4 (2B11)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
Results
Specimen Online VC WT: embryonic day 12.5 (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Pecam1 (assay MGI:5635649).
Structure Level Pattern Image Note
TS20: midgut Present Online VC WT Cxcr4 , Online VC WT Merge
Specimen Online VC 180d/d: embryonic day 12.5; Dock1tm1.1Ysfk/Dock1tm1.1Ysfk (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Pecam1 (assay MGI:5635649).
Structure Level Pattern Image Note
TS20: midgut Present Online VC 180d/d Cxcr4 , Online VC 180d/d Merge
Specimen Online VC CXCR4-/-: embryonic day 12.5; Cxcr4tm1Tng/Cxcr4tm1Tng (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Pecam1 (assay MGI:5635649).
Structure Level Pattern Image Note
TS20: midgut Absent Online VC Cxcr4-/- Merge , Online VC Cxcr4-/- Cxcr4
Specimen Online VIA: embryonic day 12.5 (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Dock1 (assay MGI:5635660).
Structure Level Pattern Image Note
TS20: midgut vascular element Present Online VIA Merge , Online VIA Cxcr4 There was co-localization of expression with Dock1 in the vascular endothelial cell lineage.
Specimen Online VIB: embryonic day 12.5 (more )
Note: Zinc fixative was used. Double labeled: green - Cxcr4; red - Dock1 (assay MGI:5635660).
Structure Level Pattern Image Note
TS20: endocardial cushion Present Online VIB Cxcr4 , Online VIB Merge There was co-localization of expression with Dock1 in the cardiac cell lineage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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