Antibody:	anti-IFITM3 (FRAGILIS ab15592)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	1B1

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	1B2

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	1E

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Single cells	2A	Cell enlarged from the intraembryonic primitive streak shows cytoplasmic spot and plasma membrane staining.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	2B	Cell enlarged from the proximal allantois shows cytoplasmic spot and plasma membrane staining.

Structure	Level	Pattern	Image	Note
TS14: embryo ectoderm	Present	Single cells	2C	Cell enlarged from posterior surface ectoderm shows apical localization.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	2D	Cell enlarged from the proximal allantois shows a small cytoplasmic spot.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Single cells	3A1	Expression is in some cells in the proximal epiblast.

Structure	Level	Pattern	Image	Note
TS9: embryo mesoderm	Present	Single cells	3A2	Expression is in posterior mesoderm.
TS9: primitive streak	Present	Single cells	3A2
TS9: extraembryonic visceral endoderm	Present	Regionally restricted	3A2	Expression is in some vessels.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Present	Widespread	3B
TS9: primitive endoderm	Present	Widespread	3B
TS9: embryo mesoderm	Present	Single cells	3B	Expression is in posterior mesoderm.

Structure	Level	Pattern	Image	Note
TS10: exocoelomic cavity	Present	Clusters	3C	All positive cells contained a large cytoplasmic spot but some cells had accompanying staining near the plasma membrane.
TS10: amniotic fold ectoderm	Present	Single cells	3C	Positive cells contained a large cytoplasmic spot with occasional plasma membrane staining.
TS10: primitive streak	Present	Single cells	3C	Expression is in cytoplasmic spots with or without plasma membrane staining to the extraembryonic extension of the primitive streak.
TS10: allantois	Present	Single cells	3C
TS10: extraembryonic component	Present	Single cells	3C	Expression is in the triangular wedge of tissue above the amnion and adjacent to the allantois-associated posterior visceral endoderm.
TS10: posterior visceral endoderm	Absent		3C	Absent in posterior endoderm.

Structure	Level	Pattern	Image	Note
TS11: exocoelomic cavity	Present	Clusters	3D
TS11: amnion ectoderm	Present	Single cells	3D	Positive cells contained a large cytoplasmic spot with occasional plasma membrane staining.
TS11: embryo	Present	Regionally restricted	3D	Positive cells in the dorsal epiblast contained a large cytoplasmic spot with occasional plasma membrane staining.
TS11: allantois	Present	Single cells	3D
TS11: primitive streak	Present	Single cells	3D
TS11: posterior visceral endoderm	Absent		3D	Absent in posterior endoderm.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	3E	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: primitive streak	Present	Single cells	3E	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: posterior visceral endoderm	Present	Regionally restricted	3E	Expression is in the primitive streak associated posterior visceral endoderm.
TS12: embryo	Present	Regionally restricted	3E	Expression is in cells in the dorsal epiblast.
TS12: yolk sac endoderm	Present	Regionally restricted	3E

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	3F1	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: primitive streak	Present	Single cells	3F1	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: posterior visceral endoderm	Present	Regionally restricted	3F1	Expression is in the primitive streak associated posterior visceral endoderm.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	3F2	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: primitive streak	Present	Single cells	3F2	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: posterior visceral endoderm	Present	Regionally restricted	3F2	Expression is in the primitive streak associated posterior visceral endoderm.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Single cells	3F3	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: primitive streak	Present	Single cells	3F3	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS12: posterior visceral endoderm	Present	Regionally restricted	3F3	Expression is in the primitive streak associated posterior visceral endoderm.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present	Single cells	3G	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS13: primitive streak	Present	Single cells	3G	Expression is in cells that extended from the distal allantoic core domain into the intraembryonic primitive streak.
TS13: hindgut diverticulum endoderm	Present	Single cells	3G
TS13: amnion	Present	Regionally restricted	3G	Expression is in cells in the amniotic ectoderm.
TS13: extraembryonic component	Present	Regionally restricted	3G	Expression is as individual cells or as small clusters of a few cells closely associated with the central allantoic arterial vessel.

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum endoderm	Present	Regionally restricted	3H	The majority of cells were in the ventral hindgut, some in the dorsal hindgut.
TS13: embryo	Present	Regionally restricted	3H	Expression is in a population at the junction between the allantois and posterior embryo.
TS13: allantois	Present	Regionally restricted	3H	Expression is in a small number of cells within the proximal allantoic midline, either as individual cells or as small clusters of a few cells. Expression is in a population at the junction between the allantois and posterior embryo.

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Present	Regionally restricted	3I	Expression is in the posterior surface ectoderm.
TS13: primordial germ cell of hindgut	Present	Single cells	3I

Structure	Level	Pattern	Image	Note
TS14: hindgut diverticulum	Present	Regionally restricted	3J	Expression is ventral.
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	3J	Expression is in the posterior mesoderm.
TS14: mesenchyme derived from somatopleure	Present		3J
TS14: tail bud	Present	Regionally restricted	3J	Expression is in the ventral ectodermal ridge.
TS14: primitive streak	Present	Regionally restricted	3J	Expression is in the posterior remnant.

Structure	Level	Pattern	Image	Note
TS14: allantois	Present	Single cells	3K	Expression is as single cells or as pairs of cells scattered throughout the allantois, from the posterior embryonic/extraembryonic junction to the portion of the allantois adjacent to the chorion.
TS14: tail bud	Present	Regionally restricted	3K	Expression is in the ventral ectodermal ridge.
TS14: primitive streak	Present	Regionally restricted	3K	Expression is in the posterior remnant.

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	4A	Expression is at the apical surface of some endodermal cells at the embryonic/extraembryonic junction of the anterior region.
TS12: future brain neural fold	Present	Regionally restricted	4A	Expression is at the apical surface of some headfold neurectoderm.

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	4B	Expression is at the apical surface of some endodermal cells at the embryonic/extraembryonic junction of the anterior region.
TS12: future brain neural fold	Present	Regionally restricted	4B	Expression is at the apical surface of some headfold neurectoderm.

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	4C	Expression is in endodermal cells overlying the developing heart.
TS12: surface ectoderm	Present	Regionally restricted	4C	Apical staining in the anterior embryo surface ectoderm.
TS12: foregut diverticulum endoderm	Present	Single cells	4C

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted	4D	Expression is at the apical surface of some headfold neurectoderm.
TS13: head mesenchyme	Present	Spotted	4D	Sporadic spots.
TS13: embryo endoderm	Present	Regionally restricted	4D	Expression is in endodermal cells overlying the developing heart.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present	Regionally restricted	4E	Expression is in a small population in the foregut opening.
TS13: embryo endoderm	Present	Regionally restricted	4E	Expression is in endodermal cells overlying the developing heart.
TS13: allantois	Present	Regionally restricted	4E	Expression is in the allantoic core domain.
TS13: surface ectoderm	Present		4E

Structure	Level	Pattern	Image	Note
TS14: foregut	Present	Regionally restricted	4F	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present	Regionally restricted	4G1	Expression is at the anterior embryo overlying neural crest tissue posterior to the nascent branchial arches.
TS14: branchial arch mesenchyme	Present	Single cells	4G1

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present	Regionally restricted	4G2	Expression is at the anterior embryo overlying neural crest tissue posterior to the nascent branchial arches.
TS14: branchial arch mesenchyme	Present	Single cells	4G2

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Single cells	4H

Structure	Level	Pattern	Image	Note
TS14: dorsal aorta	Present	Single cells	5A	Expression is in endothelial cells.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		5B1

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum	Present		5B2

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present	Regionally restricted	5C	Expression is in the loose mesenchyme in the anterior of the embryo.

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		5D

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		5E	Expression is as a large cytoplasmic spot in some hematopoietic cells of the yolk sac.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		5F
TS12: hindgut diverticulum endoderm	Present		5F
TS12: primitive streak	Present		5F
TS12: embryo	Present	Regionally restricted	5F	Expression is in the posterior dorsal epiblast.
TS12: amnion ectoderm	Present	Regionally restricted	5F	Expression is in the amniotic ectoderm adjacent to the posterior region, allantois- and primitive streak-associated posterior visceral endoderm/hindgut.

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	5G	Expression is in the anterior endoderm overlying the heart, yolk sac hematopoietic cells.
TS13: blood island	Present	Regionally restricted	5G	Expression is in endothelial cells and hematopoetic cells.

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum	Present		5H	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Regionally restricted	5I1	Expression is in the ventral ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS14: allantois	Present	Regionally restricted	5I2	Expression is in the ventral cuboidal mesothelium.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present	Single cells	6A

Structure	Level	Pattern	Image	Note
TS12: primary trophoblast giant cell	Present		6B