Gene Expression Image Detail

Reference

Figure

ID

J:198561 Mikedis MM, et al., Gene Expr Patterns. 2013 Oct;13(7):225-39

3

MGI:5634924

Image

Caption

Sections are sagittally oriented with anterior on the left and posterior on the right. (A) A1 and A2 are two different sections from the same specimen. Expression localized as a cytoplasmic spot to the proximal epiblast (epi; insets, red arrowheads) as well as to the primitive streak and posterior mesoderm (m; insets, black arrowheads). Vesicles of extraembryonic visceral endoderm (xve; red arrowheads outlined in black; A2). Black boxes designate regions magnified within insets. (B) Cytoplasmic spot to posterior mesoderm (black arrowhead), visceral endoderm (red arrowheads outlined in black), both embryonic (eve) and extraembryonic (xve). (C) Cytoplasmic spots with (inset) or without plasma membrane staining, posterior visceral endoderm (apve), amniotic ectoderm (red arrowhead). Black box within the XPS designates region magnified within inset. (D) intraembryonic posterior primitive streak (ips), dorsal epiblast (black arrows outlined in red), and amniotic ectoderm (red arrowheads). Cell localized to the allantoic bud (black arrowhead). (E) Early headfold (EHF) stage. Expression along the length of the ACD (highlighted by brackets), distal ACD (black arrowhead). Dorsal epiblast (red arrows outlined in black), IPS, and the IPS associated posterior visceral endoderm (ipve; black arrow), yolk sac extraembryonic visceral endoderm (xve) expression signal (red arrowhead outlined in black). (F) Transverse profiles of the same specimen, with ventral toward the top, and dorsal toward the bottom of each panel. Expression clustered within the ACD (F1-2, asterisk) along the proximal midline of the allantois, at 42 microns (F1) and 6 microns (F2) above the base of the allantois. Positive cells at the allantois-associated PVE (apve; black arrowhead in F2). Black arrowheads highlight expression in the IPS-associated PVE (ipve). (G) Nascent vessel of confluence (asterisk). Amniotic ectoderm (outlined in black, magnified within inset) and the nascent hindgut (hg). (H) Cytoplasmic spot with or without plasma membrane staining to the hindgut (e.g., black arrow outlined in red). Allantois (red arrow outlined in black). (I) Panel oriented with dorsal toward the top and ventral toward the bottom. Apical surface of some posterior surface ectoderm (black arrow). (J) Panel oriented with dorsal toward the top and ventral toward the bottom. Somatopleure-associated mesoderm (red arrowheads). Apical surface of the VER (black arrowhead). (K) Allantois (red arrow outlined in black), VER (black arrowhead). Panel is a magnification of the area highlighted within the inset. Scale bar in A2: 25.0 microns (A, C-E, G, H, and K); 11.5 microns (A1 inset); 11.2 microns (A2 inset); 33.3 microns (B); 6.5 microns (C inset); 30.7 microns (F); 7.9 microns (G inset); 32.1 microns (I); 31.8 microns (J); 118.5 microns (K inset).

Copyright

Reprinted with permission from Elsevier from 10.1016/j.gep.2013.04.003 Gene Expr Patterns 13: 225-39, Mikedis MM; Downs KM, Widespread but tissue-specific patterns of interferon-induced transmembrane protein 3 (IFITM3, FRAGILIS, MIL-1) in the mouse gastrula. Copyright 2013

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A1	MGI:5635440 (Ifitm3)
A2	MGI:5635440 (Ifitm3)
B	MGI:5635440 (Ifitm3)
C	MGI:5635440 (Ifitm3)
D	MGI:5635440 (Ifitm3)
E	MGI:5635440 (Ifitm3)
F1	MGI:5635440 (Ifitm3)
F2	MGI:5635440 (Ifitm3)
F3	MGI:5635440 (Ifitm3)
G	MGI:5635440 (Ifitm3)
H	MGI:5635440 (Ifitm3)
I	MGI:5635440 (Ifitm3)
J	MGI:5635440 (Ifitm3)
K	MGI:5635440 (Ifitm3)