Gene Expression Data
Assay Details
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Assay
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Reference:
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J:195181
Bijelic N, et al., Trefoil factor family protein 3 (TFF3) is present in cartilage during endochondral ossification in the developing mouse fetus. Acta Histochem. 2013 Apr;115(3):204-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5634157
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Gene symbol:
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Tff3
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Gene name:
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trefoil factor 3, intestinal
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Antibody:
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Anti-TFF3
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1:
embryonic day 17.0
(more  )
Specimen
1:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: vertebra |
Present |
Regionally restricted |
1
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Expression was detected during endochondral ossification of vertebra. Expression was in chondrocytes in the zone of proliferation, zone of hypertrophy, zone of calcification and cartilage breakdown, and in osteoblasts in the zone of ossification.
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Specimen
2A:
embryonic day 15.0
(more )
Specimen
2A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: vertebra |
Present |
Regionally restricted |
2A
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Expression was detected during endochondral ossification of vertebra. Expression was in the cytoplasm of chondrocytes in the zone of hypertrophy and calcification.
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Specimen
3A:
embryonic day 14.0
(more )
Specimen
3A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
Regionally restricted |
3A
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Expression was detected in the cytoplasm of cells in the zone of hypertrophy and zone of ossification. Apoptotic chondrocytes in the zone of calcification show signal in the whole cell.
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Specimen
4A:
embryonic day 16.0
(more )
Specimen
4A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: metatarsus |
Present |
Regionally restricted |
4A
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Expression was detected in the zone of proliferation, hypertrophy and calcification. No signal was in the zone of resting cartilage.
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Specimen
5A:
embryonic day 16.0
(more )
Specimen
5A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: rib cartilage |
Absent |
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5A
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There was no expression in non-ossified rib cartilage.
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Specimen
5B:
embryonic day 16.0
(more )
Specimen
5B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: long bone epiphysis |
Absent |
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5B
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There was no expression in unossified cartilage in the distal humeral epiphysis.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools