Antibody:	Anti-CTGF
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The fixative was Histochoice.

Structure	Level	Pattern	Image	Note
TS11: chorion	Moderate		1B
TS11: embryo ectoderm	Moderate		1B
TS11: embryo mesoderm	Weak	Regionally restricted	1B	Expression was weak in squamous endoderm.
TS11: Reichert's membrane	Absent		1B
TS11: extraembryonic visceral endoderm	Absent		1B
TS11: parietal endoderm	Absent		1B

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		1B

Structure	Level	Pattern	Image	Note
TS19: embryo	Absent		1D

Structure	Level	Pattern	Image	Note
TS21: liver	Moderate		1F
TS21: intestine	Weak		1F
TS21: lung	Weak		1F
TS21: thymus primordium	Weak		1F

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1H	There was a dramatic increased in staining levels and distribution.
TS22: dorsal root ganglion	Weak		1H
TS22: heart	Weak		1H
TS22: brain	Absent		1H	No staining was detected in the outer cortical or medullary regions of the brain.

Structure	Level	Pattern	Image	Note
TS22: arterial system	Present		2A
TS22: thymus primordium	Present		2A

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Present		2B
TS22: liver	Present		2B
TS22: lung	Present		2B
TS22: trachea	Present		2B

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	2C	Expression was strong in alveoli and bronchioles.
TS22: liver	Present	Non-Uniform	2C	Expression was strong in parenchymal cells. Weaker staining was in the outermost peritoneal layer (tunica serosa) and the underlying capsule of Glisson.
TS22: liver parenchyma	Strong		2C

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		2D
TS22: adrenal gland	Present	Non-Uniform	2D	The cortical and medullary regions stained positively, with weaker staining of the outer capsule.
TS22: adrenal cortex	Present		2D
TS22: adrenal medulla	Present		2D

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Present		2E
TS22: early nephron	Present	Regionally restricted	2E	Expression was detected in Bowman's capsule.

Structure	Level	Pattern	Image	Note
TS22: ilium cartilage condensation	Present		2F
TS22: iliac artery	Present	Regionally restricted	2F	Expression was detected in the iliac blood sinus.
TS22: perichondrium	Weak	Regionally restricted	2F	Weak expression was in perichondrial cells surrounding regions of cartilage condensation.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		3A	Expression was at the apical surface of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Present		3B	Expression was uniform in cells of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: esophagus epithelium	Present		3C	Expression was at the apical surface of the epithelium.
TS22: pharynx epithelium	Present		3C	Expression was at the apical surface of the epithelium.
TS22: thyroglossal duct	Present		3C

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		3D	Expression was uniform in cells of the epithelium. Weaker staining was in the vascular endothelial cells within the choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: remnant of Rathke's pouch	Present		3E

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle choroid plexus	Present		3F	Expression was uniform in cells of the epithelium. Weaker staining was in the vascular endothelial cells within the choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: liver	Present		4A
TS22: duodenum	Present	Regionally restricted	4A	Expression was in the prospective musculature of the duodenum.
TS22: foregut-midgut junction	Strong		4A

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		4B
TS22: midgut	Strong		4B

Structure	Level	Pattern	Image	Note
TS22: vitelline duct	Absent		4C