Antibody:	Anti-Progranulin (AF2557)
Detection system:	Secondary antibody coupled to Alexa Fluor 594
Assay notes:	Two different mouse strains were used - C57BL/6 and 129Sv/ImJ. No strain differences were detected and results from both strains were pooled. Individual specimen strain backgrounds were not provided except in the case of data from Table 2, which is indicated below.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present		2B , 2A	There was no co-label with Pcna in proliferating cells in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present		2C	There was no co-label with Nestin in immature neuronal precursors in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		2D	There was no co-label with Doublecortin in postmitotic, migrating immature neurons.

Structure	Level	Pattern	Image	Note
TS23: piriform cortex	Present		2E	There was no co-label with NeuN in layer VI of the piriform cortex.

Structure	Level	Pattern	Image	Note
TS27: piriform cortex	Present		2F	Expression was stronger and more distinct in postmitotic neurons at P0, and staining was detected in NeuN-positive mature neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present	Scattered	3A , 3C	There was co-localization with Aif1 in microglia.

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Scattered	3D	Expression was detected in microglia in the region of the developing cerebellum.

Structure	Level	Pattern	Image	Note
TS27: cerebellum white matter	Present		3E	Expression was detected in microglia migrating through the white matter into the cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		3F	Expression was detected in microglia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present			Expression was detected in microglia.
TS28: corpus callosum	Present			Expression was detected in microglia.

Structure	Level	Pattern	Image	Note
TS27: corpus callosum	Present	Regionally restricted		Expression was detected in microglia.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		3G	Expression was detected in microglia.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Absent		3H	There was a nearly complete lack of immunopositive microglia in the corpus callosum.
TS28: cerebral cortex	Present	Regionally restricted	3H	Expression was detected in cortical neurons surrounding the corpus callosum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Absent		3I

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5C , 5B	Expression co-localized with NeuN-labeled neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5E , 5F	Expression co-localized with Aif1-labeled microglia.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5I , 5H	Expression did not co-localized with Gfap-labeled astrocytes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		S1I , S1J	Co-labeling with the knock-in reporter indicates that the antibody and the knock-in reporter label the identical set of cells.

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Strong
TS28: hippocampus CA3	Strong
TS28: hypothalamus	Strong
TS28: midbrain reticular formation	Strong
TS28: prelimbic area	Strong
TS28: primary motor cortex	Strong
TS28: thalamus	Strong
TS28: visual cortex	Strong
TS28: corpus striatum	Weak
TS28: dentate gyrus	Weak
TS28: lateral septal complex	Weak
TS28: medulla oblongata	Weak
TS28: pons	Weak
TS28: cerebellum Purkinje cell layer	Moderate
TS28: hippocampus CA1	Moderate
TS28: subiculum	Moderate
TS28: substantia nigra	Moderate
TS28: superior colliculus	Moderate
TS28: cerebellum granule cell layer	Absent
TS28: cerebellum molecular layer	Absent