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Gene Expression Data
Assay Details
Assay
Reference: J:167031 Petkau TL, et al., Progranulin expression in the developing and adult murine brain. J Comp Neurol. 2010 Oct 1;518(19):3931-47
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5620500
Gene symbol: Grn
Gene name: granulin
Antibody: Anti-Progranulin (AF2557)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Two different mouse strains were used - C57BL/6 and 129Sv/ImJ. No strain differences were detected and results from both strains were pooled. Individual specimen strain backgrounds were not provided.
Results
Specimen not shown: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: brain Trace Expression was minimal at E13.5.
Specimen 1G: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex intermediate zone Present Scattered 1G
Specimen 1H: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: neocortex Present 1H
Specimen 1I: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Present 1I Expression was stronger than at earlier stages.
Specimen 1J: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Present 1J Adult cortex showed peak expression.
Specimen 4A: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: neocortex layer Present Non-Uniform 4A Expression was detected in all cell layers of the neocortex. Staining was more intense in the large pyramidal neurons of layers V and VI.
TS28: cortical layer V Strong 4A Staining was more intense in the large pyramidal neurons.
TS28: cortical layer VI Strong 4A Staining was more intense in the large pyramidal neurons.
Specimen 4C: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: hippocampus pyramidal cell layer Present Non-Uniform 4C Expression was strongest in the pyramidal cells in the CA3 region of the hippocampus and much less pronounced in the CA1 region.
TS28: CA3 pyramidal cell layer Strong 4C
TS28: CA1 pyramidal cell layer Weak 4C
Specimen not shown: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: amygdala Moderate
TS28: hypothalamus Moderate
TS28: midbrain Moderate
TS28: thalamus Moderate
TS28: brainstem Weak
Specimen 4E: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: corpus striatum Weak 4E
Specimen 4G: postnatal month 3-4 (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 4G
TS28: cerebellum granule cell layer Absent 4G
TS28: cerebellum molecular layer Absent 4G
Specimen S1A: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Strong S1A
Specimen S1B: postnatal day 7; Grntm1Blrl/Grntm1Blrl (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Absent S1B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory