Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative: 0.2% glutaraldehyde and 2% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS23: brain meninges	Strong		1A
TS23: epidermis	Strong		1A
TS23: cortical plate	Weak	Scattered	1B , 1A	Scattered cells are positive, but most are associated with blood vessels or epithelia.
TS23: blood vessel	Weak	Scattered	1A , 1B

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present	Scattered	1C	Staining in neurons was rare at E15.5.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Weak		1D	Numerous cells were labeled, but the intensity of the staining was weak in any given cell. Staining in neurons became initially apparent.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1E	Staining was stronger than at earlier stages.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1F	Adult cortex showed peak expression.

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present	Non-Uniform	4B	Expression was detected in all cell layers of the neocortex. Staining was more intense in the large pyramidal neurons of layers V and VI.
TS28: cortical layer V	Strong		4B	Staining was more intense in the large pyramidal neurons.
TS28: cortical layer VI	Strong		4B	Staining was more intense in the large pyramidal neurons.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Present	Non-Uniform	4D	Expression was strongest in the pyramidal cells in the CA3 region of the hippocampus and much less pronounced in the CA1 region.
TS28: CA3 pyramidal cell layer	Strong		4D
TS28: CA1 pyramidal cell layer	Weak		4D

Structure	Level	Pattern	Image	Note
TS28: amygdala	Moderate
TS28: hypothalamus	Moderate
TS28: midbrain	Moderate
TS28: thalamus	Moderate
TS28: brainstem	Weak

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Weak		4F

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		4H
TS28: cerebellum granule cell layer	Absent		4H
TS28: cerebellum molecular layer	Absent		4H

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		S1G

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		S1H , S1J	Co-labeling with anti-Grn antibody indicates that the knock-in reporter and the antibody label the identical set of cells.

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present
TS28: corpus striatum	Present
TS28: dentate gyrus	Present
TS28: hippocampus CA1	Present
TS28: hippocampus CA3	Present
TS28: hypothalamus	Present
TS28: lateral septal complex	Present
TS28: medulla oblongata	Present
TS28: midbrain reticular formation	Present
TS28: pons	Present
TS28: prelimbic area	Present
TS28: primary motor cortex	Present
TS28: subiculum	Present
TS28: substantia nigra	Present
TS28: superior colliculus	Present
TS28: thalamus	Present
TS28: visual cortex	Present
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum granule cell layer	Absent
TS28: cerebellum molecular layer	Absent