Antibody:	anti-DEDAF
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS8: embryo	Present		2d	Staining is nuclear.
TS8: extraembryonic component	Present		2d	Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS8: embryo	Absent		2h
TS8: extraembryonic component	Absent		2h

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present

Structure	Level	Pattern	Image	Note
TS7: embryo	Present	Scattered		Expression levels are higher in the extraembryonic compartment.
TS7: extraembryonic component	Present	Scattered		Expression levels are higher in the extraembryonic compartment.

Structure	Level	Pattern	Image	Note
TS8: embryo	Present		5a
TS8: ectoplacental cone	Present		5a
TS8: extraembryonic ectoderm	Present		5a
TS8: primary trophoblast giant cell	Present		5a
TS8: extraembryonic visceral endoderm	Present		5a	Expression is present in future fetal extraembryonic membranes.
TS8: parietal endoderm	Present		5a	Expression is present in future fetal extraembryonic membranes.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		5b	Expression is present in the epiblast.
TS9: primary trophoblast giant cell	Strong		5b
TS9: ectoplacental cone	Present		5b
TS9: extraembryonic ectoderm	Present		5b
TS9: extraembryonic visceral endoderm	Present		5b	Expression is present in future fetal extraembryonic membranes.
TS9: parietal endoderm	Present		5b	Expression is present in future fetal extraembryonic membranes.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		5c
TS11: embryo mesoderm	Present		5c

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Strong	Uniform	5d

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		5e
TS13: chorion	Present		5e

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present		5f	Expression is present in the lining.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present		5g
TS13: neural ectoderm	Present		5g	Expression is present in the neuroepithelium of the closing neural plate.

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Strong		5h

Structure	Level	Pattern	Image	Note
TS21: extraembryonic component	Strong		5i	Expression is present in the fetal but not maternal compartments of the placenta.
TS21: labyrinthine zone	Present		5i

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Moderate	Non-Uniform	7a	Expression is present in the neuroepithelium of the closing neural plate. It is enriched at the median hinge point.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	7b	Expression is present in the outermost cell layers of the dorsal neopallium.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present	Regionally restricted	7c	Expression is present in the ventral diencephalon.
TS16: Rathke's pouch	Present		7c

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		7d

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted		Expression is present in the dorsal hindbrain.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	7f	There is marked staining in the preplate.
TS19: telencephalon ventricular layer	Absent		7f

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present			Expression is present in postmitotic neurons.
TS20: cerebral cortex ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Strong		7h
TS24: cortical plate	Strong		7h
TS24: cerebral cortex subventricular zone	Moderate		7h
TS24: cerebral cortex ventricular layer	Absent		7h

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Strong		7i
TS24: cortical plate	Strong		7i

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Strong		7j

Structure	Level	Pattern	Image	Note
TS27: forebrain	Present	Spotted		Expression is present in many outer cortical neurons.
TS27: midbrain	Present	Spotted		Expression is present in many outer cortical neurons.

Structure	Level	Pattern	Image	Note
TS28: cranial ganglion	Present
TS28: peripheral nervous system	Present			Expression is present in peripheral ganglia.
TS28: head	Present	Regionally restricted		Expression is present in cephalic mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present		7k	Expression is present in neuroblasts.

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present		7l