Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:114716
Ellsworth BS, et al., FOXL2 in the pituitary: molecular, genetic, and developmental analysis. Mol Endocrinol. 2006 Nov;20(11):2796-805
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5618413
|
|
Gene symbol:
|
Foxl2
|
|
Gene name:
|
forkhead box L2
|
|
Antibody:
|
Anti-Foxl2 (C terminus)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
|
|
|
Results
|
Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future pituitary |
Present |
Regionally restricted |
1C
|
Expression was detected in the developing anterior lobe of the pituitary. Expression coincides with alpha-GSU (Cga) spatially and temporally.
|
Specimen
1E:
embryonic day 12.5
(more )
Specimen
1E:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: adenohypophysis |
Present |
|
1E
|
Expression was detected below a band of nonproliferating, undifferentiated cells and was colocalized with nonproliferating, differentiating cells. Expression coincides with alpha-GSU (Cga) spatially and temporally.
|
Specimen
1G:
embryonic day 12.5
(more )
Note: Double labeled with BrdU (red).
Specimen
1G:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled with BrdU (red).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: adenohypophysis |
Present |
Regionally restricted |
1G
|
Expression did not colocalize with BrdU and was confined to the nonproliferating cells of the anterior lobe.
|
Specimen
1H:
embryonic day 18.5
(more )
Note: Double labeled with BrdU (red).
Specimen
1H:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled with BrdU (red).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: adenohypophysis |
Present |
Regionally restricted |
1H
|
Expression did not colocalize with BrdU and was confined to the nonproliferating cells of the anterior lobe.
|
Specimen
4A:
embryonic day 12.5
(more )
Specimen
4A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Lhx3tm1Lmgd/Lhx3tm1Lmgd
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4C:
embryonic day 18.5
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J
|
|
Mutant Allele(s):
|
Lhx3tm1Lmgd/Lhx3tm1Lmgd
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4E:
embryonic day 12.5
(more )
Specimen
4E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4F:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J * CF-1
|
|
Mutant Allele(s):
|
Lhx4tm1Ssp/Lhx4tm1Ssp
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4G:
embryonic day 16.5
(more )
Specimen
4G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4H:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J * CF-1
|
|
Mutant Allele(s):
|
Lhx4tm1Ssp/Lhx4tm1Ssp
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: adenohypophysis |
Present |
|
4H
|
Expression level in mutant mice recovered by E16.5.
|
Specimen
4I:
embryonic day 12.5
(more )
Specimen
4I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4J:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Mutant Allele(s):
|
Prop1df/Prop1df
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: adenohypophysis |
Present |
|
4J
|
There were no obvious alterations in the level or spatial expression pattern in mutants relative to wild-type.
|
Specimen
4K:
embryonic day 15.5
(more )
Specimen
4K:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4L:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Mutant Allele(s):
|
Prop1df/Prop1df
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: adenohypophysis |
Present |
|
4L
|
There were no obvious alterations in the level or spatial expression pattern in mutants relative to wild-type.
|
|
Analysis Tools