Gene Expression Data
Assay Details
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Assay
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Reference:
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J:218012
Rutlin M, et al., The cellular and molecular basis of direction selectivity of Adelta-LTMRs. Cell. 2014 Dec 18;159(7):1640-51
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5617192
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Gene symbol:
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Ntrk2
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Gene name:
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neurotrophic tyrosine kinase, receptor, type 2
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Reporter:
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GFP
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Antibody:
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anti-GFP (Aves)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The authors described two anti-GFP antibodies but did not detail which was used to obtain these data; it is possible MGI:4354373 was used instead.
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Results
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Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6J * DBA/2J
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Mutant Allele(s):
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Bdnftm1Krj/Bdnf+
Ntrk2tm2.1Ddg/Ntrk2+
Tg(KRT5-cre)5132Jlj/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal nerve |
Present |
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5A
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Expression is present in alpha-delta low-threshold mechanoreceptor lanceolate endings associated with hair follicles.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6J * DBA/2J
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Mutant Allele(s):
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Bdnftm1Krj/Bdnftm1Krj
Ntrk2tm2.1Ddg/Ntrk2+
Tg(KRT5-cre)5132Jlj/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal nerve |
Present |
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5B
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Expression is present in alpha-delta low-threshold mechanoreceptor lanceolate endings associated with hair follicles. These endings lack polarization in the mutant.
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Specimen
S5A:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ntrk2tm2.1Ddg/Ntrk2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Present |
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S5A
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Expression is present in alpha-delta low-threshold mechanoreceptors.
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Specimen
S5B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ntrk2tm2.1Ddg/Ntrk2tm2.1Ddg
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Trace |
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S5B
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Expression is present in alpha-delta low-threshold mechanoreceptors. These neurons are nearly completely lost in the mutant.
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Specimen
S5C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac
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Mutant Allele(s):
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Bdnftm1Krj/Bdnftm1Krj
Ntrk2tm2.1Ddg/Ntrk2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Present |
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S5C
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Expression is present in alpha-delta low-threshold mechanoreceptors. There is a 66 percent reduction in the number of these neurons in the mutant compared to littermate controls.
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Specimen
S5E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C3H * C57BL/6
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Mutant Allele(s):
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Bdnftm1Krj/Bdnftm1Krj
Ntrk2tm2.1Ddg/Ntrk2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5F:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C3H * C57BL/6
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Mutant Allele(s):
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Bdnftm1Krj/Bdnftm1Krj
Ntrk2tm2.1Ddg/Ntrk2+
Tg(T-cre)1Lwd/0
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Present |
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S5F
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Expression is present in thoracic-level neurons. There is a 50 percent reduction in the number of these neurons in the mutant compared to littermate controls.
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Specimen
S5G:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6J * CBA/J
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Mutant Allele(s):
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Bdnftm1Krj/Bdnftm1Krj
Ntrk2tm2.1Ddg/Ntrk2+
H2az2Tg(Wnt1-cre)11Rth/H2az2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Present |
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S5G
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Expression is present in thoracic-level neurons. The numbers of these neurons are comparable in the mutant and littermate controls.
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Specimen
S5H:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6J * DBA/2J
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Mutant Allele(s):
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Bdnftm1Krj/Bdnftm1Krj
Ntrk2tm2.1Ddg/Ntrk2+
Tg(KRT5-cre)5132Jlj/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
Present |
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S5H
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Expression is present in thoracic-level neurons. The numbers of these neurons are comparable in the mutant and littermate controls.
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