Mbd5 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212073 Camarena V, et al., Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome. EMBO Mol Med. 2014;6(8):1003-15
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5615205
Gene symbol:	Mbd5
Gene name:	methyl-CpG binding domain protein 5

Reporter:	lacZ
	Direct Detection

Results

3A ©

Specimen 3A: postnatal month 1; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Image	Note
TS28: cerebellum granule cell layer	Strong	3A
TS28: hypothalamus	Strong	3A
TS28: main olfactory bulb granule cell layer	Strong	3A
TS28: main olfactory bulb mitral cell layer	Strong	3A
TS28: thalamus	Strong	3A
TS28: main olfactory bulb	Present	3A	Expression was stronger in the anterior olfactory nuclei.
TS28: cerebellum	Present	3A
TS28: cerebral cortex	Present	3A
TS28: corpus striatum	Present	3A
TS28: dentate gyrus	Present	3A
TS28: hippocampus CA1	Present	3A
TS28: hippocampus CA2	Present	3A
TS28: hippocampus CA3	Present	3A

3B merged ©

3B X-gal ©

Specimen 3B: postnatal month 1; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)
Note: Triple labeled: black - Mbd5 reporter; red - Rbfox3 (assay MGI:5615210); green - Gfap (assay MGI:5615211).

Structure	Level	Pattern	Image	Note
TS28: brain	Present		3B X-gal , 3B merged	Expression is in neurons, not astrocytes.

3C top ©

Specimen 3C top: postnatal month 1; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis	Present		3C top
TS28: adenohypophysis pars intermedia	Present		3C top

3C bottom ©

Specimen 3C bottom: postnatal month 1; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

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Structure	Level	Pattern	Image	Note
TS28: adenohypophysis	Present		3C bottom
TS28: adenohypophysis pars intermedia	Present		3C bottom

S2A Kidney ©

Specimen S2A Kidney: postnatal; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

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Structure	Level	Pattern	Image	Note
TS28: metanephros	Present		S2A Kidney

S2A Liver ©

Specimen S2A Liver: postnatal; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Weak		S2A Liver

S2A Lung ©

Specimen S2A Lung: postnatal; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

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Structure	Level	Pattern	Image	Note
TS28: lung	Weak		S2A Lung

S2A Spleen ©

Specimen S2A Spleen: postnatal; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: spleen	Weak		S2A Spleen

S2A Heart ©

Specimen S2A Heart: postnatal; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: heart	Present		S2A Heart

Specimen S2A S. Intestine: postnatal; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: small intestine	Weak		S2A S. Intestine

S2B E5.5 ©

Specimen S2B E5.5: embryonic day 5.5; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS8: embryo	Present		S2B E5.5

S2B E15 ©

Specimen S2B E15: embryonic day 15.0; Mbd5^{Gt(Ayu21-B205)Imeg}/Mbd5⁺ (more

)

Structure	Level	Image
TS23: forebrain	Strong	S2B E15
TS23: heart	Strong	S2B E15
TS23: hindbrain	Strong	S2B E15
TS23: midbrain	Strong	S2B E15
TS23: spinal cord	Strong	S2B E15

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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