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Gene Expression Data
Assay Details
Assay
Reference: J:203014 Chi L, et al., Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis. Gene Expr Patterns. 2013 Dec;13(8):319-27
Assay type: RNA in situ
MGI Accession ID: MGI:5614020
Gene symbol: Nsun3
Gene name: NOL1/NOP2/Sun domain family member 3
Probe: Nsun3 cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Embryos were post-fixed with 4% PFA and 0.2% glutaraldehyde.
Results
Specimen 2A: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo Present 2A Broad expression.
TS11: allantois Strong 2A
TS11: chorion Strong 2A
TS11: extraembryonic ectoderm Strong 2A
TS11: neural ectoderm Strong Regionally restricted 2A Expression is in the anterior neuroectoderm.

Specimen 2B: embryonic day 8.5 (more )
Note: (C) is a higher magnification of the boxed area in (B).
Structure Level Pattern Image Note
TS13: embryo Moderate 2B , 2C Constitutive expression.
TS13: sinus venosus Strong 2B , 2C
TS13: somite Present 2C , 2B
TS13: future brain neural fold Strong 2B
TS13: heart Strong 2B

Specimen 2D: embryonic day 9.5 (more )
Note: (E) is a higher magnification of the boxed area in (D).
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 2D , 2E
TS15: 2nd branchial arch Present 2D , 2E
TS15: dorsal aorta Present 2D , 2E
TS15: facio-acoustic preganglion complex Present 2D , 2E
TS15: fronto-nasal process Present 2D , 2E
TS15: future forebrain Present 2D , 2E
TS15: lens placode Present 2D , 2E
TS15: trigeminal preganglion Present 2D , 2E
TS15: otic pit Present Regionally restricted 2E , 2D Expression is in the placodes.
TS15: future hindbrain Weak 2D , 2E
TS15: future midbrain Weak 2D , 2E
TS15: heart Weak 2D , 2E
TS15: forelimb bud Present 2D
TS15: somite Present 2D

Specimen 2F: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 2F
TS15: 2nd branchial arch Present 2F
TS15: forelimb bud Present 2F
TS15: fronto-nasal process Present 2F
TS15: future forebrain Present 2F
TS15: lens placode Present 2F
TS15: neural tube Present 2F
TS15: future midbrain Weak 2F
TS15: heart Weak 2F

Specimen 2G: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: forelimb bud Present 2G
TS15: lens placode Present 2G
TS15: future hindbrain Weak 2G
TS15: future midbrain Weak 2G

Specimen 2H: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: fronto-nasal process Present 2H
TS15: future forebrain Present 2H
TS15: somite Present 2H
TS15: future hindbrain Weak 2H
TS15: future midbrain Weak 2H
TS15: heart Weak 2H

Specimen 2I: embryonic day 10.5 (more )
Note: (J,K) are a higher magnifications of the boxed areas in (I).
Structure Level Pattern Image Note
TS17: 1st branchial arch Present 2I , 2J
TS17: 2nd branchial arch Present 2I , 2J
TS17: 3rd branchial arch Present 2I , 2J
TS17: acoustic ganglion Present 2I , 2J
TS17: facial ganglion Present 2I , 2J
TS17: forebrain Present 2I , 2J
TS17: midbrain-hindbrain junction Present 2I , 2J
TS17: olfactory epithelium Present 2I , 2J
TS17: trigeminal ganglion Present 2I , 2J
TS17: neural tube Present Regionally restricted 2I , 2K Expression is at the midline.
TS17: blood vessel Present Regionally restricted 2I , 2K Expression is in intersomitic vessels.
TS17: hindlimb bud Present 2I
TS17: liver Present 2I
TS17: hindbrain Weak 2J , 2I
TS17: midbrain Weak 2J , 2I
TS17: heart Weak 2I
TS17: somite Weak 2K , 2I
TS17: forelimb bud Present 2I , 2K
TS17: dorsal aorta Present 2I , 2K

Specimen 2L: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch Present 2L
TS17: forebrain Present 2L
TS17: olfactory epithelium Present 2L
TS17: heart Weak 2L
TS17: hindbrain Weak 2L
TS17: midbrain Weak 2L
TS17: somite Weak 2L

Specimen Table 2 E7.5: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: allantois Present
TS11: chorion Present
TS11: extraembryonic ectoderm Present
TS11: neural ectoderm Present Regionally restricted Expression is in the anterior neuroectoderm.

Specimen Table 2 E8.5: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: future brain neural fold Present
TS13: heart Present
TS13: sinus venosus Present
TS13: somite Present

Specimen Table 2 E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: 1st branchial arch Present
TS15: 2nd branchial arch Present
TS15: dorsal aorta Present
TS15: facio-acoustic preganglion complex Present
TS15: forelimb bud Present
TS15: fronto-nasal process Present
TS15: future forebrain Present
TS15: lens placode Present
TS15: somite Present
TS15: trigeminal preganglion Present
TS15: otic pit Present Regionally restricted Expression is in the placodes.

Specimen Table 2 E10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch Present
TS17: 2nd branchial arch Present
TS17: 3rd branchial arch Present
TS17: acoustic ganglion Present
TS17: dorsal aorta Present
TS17: facial ganglion Present
TS17: forebrain Present
TS17: forelimb bud Present
TS17: hindlimb bud Present
TS17: lens pit Present
TS17: liver Present
TS17: midbrain-hindbrain junction Present
TS17: neural tube Present
TS17: olfactory epithelium Present
TS17: somite Present
TS17: trigeminal ganglion Present
TS17: blood vessel Present Regionally restricted Expression is in intersomitic vessels.
TS17: otocyst Present Regionally restricted Expression is in the placodes.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory